{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T23:30:04Z","timestamp":1778974204429,"version":"3.51.4"},"reference-count":64,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,3,14]],"date-time":"2018-03-14T00:00:00Z","timestamp":1520985600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["P28218\u2010B22"],"award-info":[{"award-number":["P28218\u2010B22"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Many soluble and integral membrane proteins fold in the endoplasmic reticulum (<jats:styled-content style=\"fixed-case\">ER<\/jats:styled-content>) with the help of chaperones and folding factors. Despite these efforts, protein folding is intrinsically error prone and amino acid changes, alterations in post\u2010translational modifications or cellular stress can cause protein misfolding. Folding\u2010defective non\u2010native proteins are cleared from the <jats:styled-content style=\"fixed-case\">ER<\/jats:styled-content> and typically undergo <jats:styled-content style=\"fixed-case\">ER<\/jats:styled-content>\u2010associated degradation (<jats:styled-content style=\"fixed-case\">ERAD<\/jats:styled-content>). Here, we investigated whether different misfolded glycoproteins require the same set of <jats:styled-content style=\"fixed-case\">ERAD<\/jats:styled-content> factors and are directed to <jats:styled-content style=\"fixed-case\">HRD<\/jats:styled-content>1 complex\u2010mediated degradation in plants. We generated a series of glycoprotein <jats:styled-content style=\"fixed-case\">ERAD<\/jats:styled-content> substrates harboring a misfolded domain from Arabidopsis <jats:styled-content style=\"fixed-case\">STRUBBELIG<\/jats:styled-content> or the <jats:styled-content style=\"fixed-case\">BRASSINOSTEROID INSENSITVE<\/jats:styled-content> 1 receptor fused to different membrane anchoring regions. We show that single pass and multispanning <jats:styled-content style=\"fixed-case\">ERAD<\/jats:styled-content> substrates are subjected to glycan\u2010dependent degradation by the <jats:styled-content style=\"fixed-case\">HRD<\/jats:styled-content>1 complex. However, the presence of a powerful <jats:styled-content style=\"fixed-case\">ER<\/jats:styled-content> exit signal in the multispanning <jats:styled-content style=\"fixed-case\">ERAD<\/jats:styled-content> substrates causes competition with <jats:styled-content style=\"fixed-case\">ER<\/jats:styled-content> quality control and targeting of misfolded glycoproteins to the vacuole. Our results demonstrate that the same machinery is used for degradation of topologically different misfolded glycoproteins in the <jats:styled-content style=\"fixed-case\">ER<\/jats:styled-content> of plants.<\/jats:p>","DOI":"10.1111\/tpj.13851","type":"journal-article","created":{"date-parts":[[2018,2,3]],"date-time":"2018-02-03T11:52:51Z","timestamp":1517658771000},"page":"246-259","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["The glycan\u2010dependent <scp>ERAD<\/scp> machinery degrades topologically diverse misfolded proteins"],"prefix":"10.1111","volume":"94","author":[{"given":"Yun\u2010Ji","family":"Shin","sequence":"first","affiliation":[{"name":"Department of Applied Genetics and Cell Biology University of Natural Resources and Life Sciences Muthgasse 18 Vienna A\u20101190 Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrike","family":"Vavra","sequence":"additional","affiliation":[{"name":"Department of Applied Genetics and Cell Biology University of Natural Resources and Life Sciences Muthgasse 18 Vienna A\u20101190 Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christiane","family":"Veit","sequence":"additional","affiliation":[{"name":"Department of Applied Genetics and Cell Biology University of Natural Resources and Life Sciences Muthgasse 18 Vienna A\u20101190 Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8764-6530","authenticated-orcid":false,"given":"Richard","family":"Strasser","sequence":"additional","affiliation":[{"name":"Department of Applied Genetics and Cell Biology University of Natural Resources and Life Sciences Muthgasse 18 Vienna A\u20101190 Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,3,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0854.2002.31102.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aac4354"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.05.048"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.120.4.961"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200910042"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.1998.00208.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.2003.01728.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0173924"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nplants.2016.94"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2016.12.011"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2793"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200809198"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.4161\/psb.25129"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.112.108696"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)55439-9"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2011.04688.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.013052"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/mp\/ssn066"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.112.096057"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e03-02-0101"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2010.04322.x"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.108.061879"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1119173109"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2012.00067"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-012-9891-4"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20141057"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.114.123216"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2007.05.015"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0015532"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E06-08-0696"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2017.04.066"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.109.072363"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2014.00162"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2010.181"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1511724112"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.111.177816"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M709316200"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erv229"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.105.036665"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12685"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1703682114"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0960-7412.2002.01252.x"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.105.036400"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.06.026"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/mp\/ssq082"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2008.00841.x"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.112.210757"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.12671"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.01.018"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201602010"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(04)00150-4"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1013251108"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/mp\/sss042"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2004.02141.x"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M301080200"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1991.tb16166.x"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0019730"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1080\/15592324.2016.1171451"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200309132"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200106123"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2546"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/ern143"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E15-06-0361"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.08.036"}],"container-title":["The Plant Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftpj.13851","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tpj.13851","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T22:47:11Z","timestamp":1694299631000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tpj.13851"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3,14]]},"references-count":64,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,4]]}},"alternative-id":["10.1111\/tpj.13851"],"URL":"https:\/\/doi.org\/10.1111\/tpj.13851","archive":["Portico"],"relation":{},"ISSN":["0960-7412","1365-313X"],"issn-type":[{"value":"0960-7412","type":"print"},{"value":"1365-313X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,3,14]]},"assertion":[{"value":"2017-11-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-01-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-03-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}