{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T06:01:29Z","timestamp":1791957689348,"version":"4.3.4"},"reference-count":59,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,3,31]],"date-time":"2018-03-31T00:00:00Z","timestamp":1522454400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003825","name":"Magyar Tudom\u00e1nyos Akad\u00e9mia","doi-asserted-by":"publisher","award":["LP2014\/19"],"award-info":[{"award-number":["LP2014\/19"]}],"id":[{"id":"10.13039\/501100003825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Sulphur limitation may restrain cell growth and viability. In the green alga <jats:italic>Chlamydomonas reinhardtii<\/jats:italic>, sulphur limitation may induce H<jats:sub>2<\/jats:sub> production lasting for several days, which can be exploited as a renewable energy source. Sulphur limitation causes a large number of physiological changes, including the inactivation of photosystem\u00a0<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">PSII<\/jats:styled-content>), leading to the establishment of hypoxia, essential for the increase in hydrogenase expression and activity. The inactivation of <jats:styled-content style=\"fixed-case\">PSII<\/jats:styled-content> has long been assumed to be caused by the sulphur\u2010limited turnover of its reaction center protein PsbA. Here we reinvestigated this issue in detail and show that: (i) upon transferring <jats:italic>Chlamydomonas<\/jats:italic> cells to sulphur\u2010free media, the cellular sulphur content decreases only by about 25%; (ii) as demonstrated by lincomycin treatments, PsbA has a significant turnover, and other photosynthetic subunits, namely RbcL and <jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content>43, are degraded more rapidly than PsbA. On the other hand, sulphur limitation imposes oxidative stress early on, most probably involving the formation of singlet oxygen in <jats:styled-content style=\"fixed-case\">PSII<\/jats:styled-content>, which leads to an increase in the expression of <jats:styled-content style=\"fixed-case\">GDP<\/jats:styled-content>\u2010L\u2010galactose phosphorylase, playing an essential role in ascorbate biosynthesis. When accumulated to the millimolar concentration range, ascorbate may inactivate the oxygen\u2010evolving complex and provide electrons to <jats:styled-content style=\"fixed-case\">PSII<\/jats:styled-content>, albeit at a low rate. In the absence of a functional donor side and sufficient electron transport, <jats:styled-content style=\"fixed-case\">PSII<\/jats:styled-content> reaction centers are inactivated and degraded. We therefore demonstrate that the inactivation of <jats:styled-content style=\"fixed-case\">PSII<\/jats:styled-content> is a complex and multistep process, which may serve to mitigate the damaging effects of sulphur limitation.<\/jats:p>","DOI":"10.1111\/tpj.13878","type":"journal-article","created":{"date-parts":[[2018,2,23]],"date-time":"2018-02-23T20:11:06Z","timestamp":1519416666000},"page":"548-561","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":63,"title":["The mechanism of photosystem\u2010<scp>II<\/scp> inactivation during sulphur deprivation\u2010induced H<sub>2<\/sub> production in <i>Chlamydomonas reinhardtii<\/i>"],"prefix":"10.1111","volume":"94","author":[{"given":"Val\u00e9ria","family":"Nagy","sequence":"first","affiliation":[{"name":"Institute of Plant Biology Biological Research Centre Hungarian Academy of Sciences Szeged Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9","family":"Vidal\u2010Meireles","sequence":"additional","affiliation":[{"name":"Institute of Plant Biology Biological Research Centre Hungarian Academy of Sciences Szeged Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Podmaniczki","sequence":"additional","affiliation":[{"name":"Institute of Plant Biology Biological Research Centre Hungarian Academy of Sciences Szeged Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kl\u00e1ra","family":"Szentmih\u00e1lyi","sequence":"additional","affiliation":[{"name":"Institute of Materials and Environmental Chemistry Research Centre for Natural Sciences Hungarian Academy of Sciences Budapest Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00e1bor","family":"R\u00e1khely","sequence":"additional","affiliation":[{"name":"Department of Biotechnology University of Szeged Szeged Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Zsigmond","sequence":"additional","affiliation":[{"name":"Institute of Plant Biology Biological Research Centre Hungarian Academy of Sciences Szeged Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00e1szl\u00f3","family":"Kov\u00e1cs","sequence":"additional","affiliation":[{"name":"Institute of Plant Biology Biological Research Centre Hungarian Academy of Sciences Szeged Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Szilvia Z.","family":"T\u00f3th","sequence":"additional","affiliation":[{"name":"Institute of Plant Biology Biological Research Centre Hungarian Academy of Sciences Szeged 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