{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T13:36:09Z","timestamp":1791725769601,"version":"4.3.4"},"reference-count":63,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,10,8]],"date-time":"2018-10-08T00:00:00Z","timestamp":1538956800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004189","name":"Max-Planck-Gesellschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004189","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2018,12]]},"abstract":"Summary<\/jats:title>The phenylpropanoid pathway leads to the production of many important plant secondary metabolites including lignin, chlorogenic acids, flavonoids, and phenolic glycosides. Early studies have demonstrated that flavonoid biosynthesis is transcriptionally regulated, often by a MYB<\/jats:styled-content>, bHLH<\/jats:styled-content>, and WDR<\/jats:styled-content> transcription factor complex. In poplar, several R2R3 MYB<\/jats:styled-content> transcription factors are known to be involved in flavonoid biosynthesis. Previous work determined that poplar MYB<\/jats:styled-content>134<\/jats:italic> and MYB<\/jats:styled-content>115<\/jats:italic> are major activators of the proanthocyanidin pathway, and also induce the expression of repressor\u2010like MYB<\/jats:styled-content> transcription factors. Here we characterize two new repressor MYB<\/jats:styled-content>s, poplar MYB<\/jats:styled-content>165<\/jats:italic> and MYB<\/jats:styled-content>194<\/jats:italic>, paralogs which comprise a subgroup of R2R3\u2010MYB<\/jats:styled-content>s distinct from previously reported poplar repressors. Both MYB<\/jats:styled-content>165<\/jats:italic> and MYB<\/jats:styled-content>194<\/jats:italic> repressed the activation of flavonoid promoters by MYB<\/jats:styled-content>134<\/jats:italic> in transient activation assays, and both interacted with a co\u2010expressed bHLH<\/jats:styled-content> transcription factor, bHLH<\/jats:styled-content>131, in yeast two\u2010hybrid assays. Overexpression of MYB<\/jats:styled-content>165<\/jats:italic> and MYB<\/jats:styled-content>194<\/jats:italic> in hybrid poplar resulted in greatly reduced accumulation of several phenylpropanoids including anthocyanins, proanthocyanidins, phenolic glycosides, and hydroxycinnamic acid esters. Transcriptome analysis of MYB<\/jats:styled-content>165<\/jats:italic>\u2010 and MYB<\/jats:styled-content>194<\/jats:italic>\u2010overexpressing poplars confirmed repression of many phenylpropanoid enzyme genes. In addition, other MYB<\/jats:styled-content> genes as well as several shikimate pathway enzyme genes were downregulated by MYB<\/jats:styled-content>165<\/jats:italic>\u2010overexpression. By contrast, leaf aromatic amino acid concentrations were greater in MYB<\/jats:styled-content>165<\/jats:italic>\u2010overexpressing poplars. Our findings indicate that MYB<\/jats:styled-content>165<\/jats:italic> is a major repressor of the flavonoid and phenylpropanoid pathway in poplar, and may also affect the shikimate pathway. The coordinated action of repressor and activator MYB<\/jats:styled-content>s could be important for the fine tuning of proanthocyanidin biosynthesis during development or following stress.<\/jats:p>","DOI":"10.1111\/tpj.14081","type":"journal-article","created":{"date-parts":[[2018,9,3]],"date-time":"2018-09-03T15:50:05Z","timestamp":1535989805000},"page":"949-965","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":179,"title":["Two R2R3\u2010MYB<\/scp> proteins are broad repressors of flavonoid and phenylpropanoid metabolism in poplar"],"prefix":"10.1111","volume":"96","author":[{"given":"Dawei","family":"Ma","sequence":"first","affiliation":[{"name":"Centre for Forest Biology & Department of Biology University of Victoria 3800 Finnerty Road Victoria BC V8P 5C2 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Reichelt","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Max\u2010Planck Institute for Chemical Ecology Hans\u2010Kn\u00f6ll Strasse 8 07745 Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuko","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Centre for Forest Biology & Department of Biology University of Victoria 3800 Finnerty Road Victoria BC V8P 5C2 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Gershenzon","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Max\u2010Planck Institute for Chemical Ecology Hans\u2010Kn\u00f6ll Strasse 8 07745 Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. Peter","family":"Constabel","sequence":"additional","affiliation":[{"name":"Centre for Forest Biology & Department of Biology University of Victoria 3800 Finnerty Road Victoria BC V8P 5C2 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