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An important pre\u2010condition for the introduction of a functional C<jats:sub>4<\/jats:sub> cycle is the photosynthetic activation of the C<jats:sub>3<\/jats:sub> bundle sheath by increasing its volume and organelle number. Therefore, to engineer C<jats:sub>4<\/jats:sub> photosynthesis into existing C<jats:sub>3<\/jats:sub> crops, information about genes that control the bundle sheath cell size and organelle content is needed. However, very little information is known about the genes that could be manipulated to create a more C<jats:sub>4<\/jats:sub>\u2013like bundle sheath. To this end, an ethylmethanesulfonate (<jats:styled-content style=\"fixed-case\">EMS)<\/jats:styled-content>\u2010based forward genetic screen was established in the Brassicaceae C<jats:sub>3\u00a0<\/jats:sub>species <jats:italic>Arabidopsis thaliana<\/jats:italic>. To ensure a high\u2010throughput primary screen, the bundle sheath cells of <jats:italic>A.\u00a0thaliana<\/jats:italic> were labeled using a luciferase (<jats:styled-content style=\"fixed-case\">LUC<\/jats:styled-content>68) or by a chloroplast\u2010targeted green fluorescent protein (<jats:styled-content style=\"fixed-case\">sGFP<\/jats:styled-content>) reporter using a bundle sheath specific promoter. The signal strengths of the reporter genes were used as a proxy to search for mutants with altered bundle sheath anatomy. Here, we show that our genetic screen predominantly identified mutants that were primarily affected in the architecture of the vascular bundle, and led to an increase in bundle sheath volume. By using a mapping\u2010by\u2010sequencing approach the genomic segments that contained mutated candidate genes were identified.<\/jats:p>","DOI":"10.1111\/tpj.14165","type":"journal-article","created":{"date-parts":[[2018,11,17]],"date-time":"2018-11-17T13:53:53Z","timestamp":1542462833000},"page":"984-995","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Reporter\u2010based forward genetic screen to identify bundle sheath anatomy mutants in <i>A.\u00a0thaliana<\/i>"],"prefix":"10.1111","volume":"97","author":[{"given":"Florian","family":"D\u00f6ring","sequence":"first","affiliation":[{"name":"Institute of Plant Molecular and Developmental Biology Heinrich\u2010Heine University Universit\u00e4tsstrasse 1 40225 Duesseldorf Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kumari","family":"Billakurthi","sequence":"additional","affiliation":[{"name":"Institute of Plant Molecular and Developmental Biology Heinrich\u2010Heine University Universit\u00e4tsstrasse 1 40225 Duesseldorf Germany"},{"name":"Cluster of Excellence on Plant Sciences \u2018From Complex Traits towards Synthetic Modules\u2019 40225 Duesseldorf and 50923 Cologne Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Udo","family":"Gowik","sequence":"additional","affiliation":[{"name":"Institute of Plant Molecular and Developmental Biology Heinrich\u2010Heine University Universit\u00e4tsstrasse 1 40225 Duesseldorf Germany"},{"name":"Department of Biology and Environmental Sciences Carl Von Ossietzky University Ammerlaender Heerstrasse 114 26129 Oldenburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefanie","family":"Sultmanis","sequence":"additional","affiliation":[{"name":"Department of Ecology and Evolutionary Biology The University of Toronto Toronto ON M5S 3B2 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roxana","family":"Khoshravesh","sequence":"additional","affiliation":[{"name":"Department of Ecology and Evolutionary Biology The University of Toronto Toronto ON M5S 3B2 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shipan","family":"Das Gupta","sequence":"additional","affiliation":[{"name":"Institute of Plant Molecular and Developmental Biology Heinrich\u2010Heine University Universit\u00e4tsstrasse 1 40225 Duesseldorf Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tammy L.","family":"Sage","sequence":"additional","affiliation":[{"name":"Department of Ecology and Evolutionary Biology The University of Toronto Toronto ON M5S 3B2 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Westhoff","sequence":"additional","affiliation":[{"name":"Institute of Plant Molecular and Developmental Biology Heinrich\u2010Heine University Universit\u00e4tsstrasse 1 40225 Duesseldorf Germany"},{"name":"Cluster of Excellence on Plant Sciences \u2018From Complex Traits towards Synthetic Modules\u2019 40225 Duesseldorf and 50923 Cologne Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,1,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2095"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.110.074682"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.113.113118"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.12502"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1146\/annurev.pp.44.060193.001345","article-title":"Strategies of phloem loading","volume":"44","author":"Bel A.J.E. 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