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To investigate the interaction of <jats:italic>R.\u00a0solanacearum<\/jats:italic> and its tomato (<jats:italic>Solanum lycopersicum<\/jats:italic>) plant host, a comparative proteomic analysis was conducted in tomato stems inoculated with highly and mildly aggressive <jats:italic>R.\u00a0solanacearum<\/jats:italic> isolates (RsH and RsM, respectively). The results indicated a significant alteration of the methionine cycle (<jats:styled-content style=\"fixed-case\">MTC<\/jats:styled-content>) and downregulation of \u03b3\u2010aminobutyric acid (<jats:styled-content style=\"fixed-case\">GABA<\/jats:styled-content>) biosynthesis. Furthermore, transcriptome profiling of two key tissues (stem and root) at three stages (0, 3 and 5 days post\u2010inoculation) with RsH in resistant and susceptible tomato plants is presented. Transcript profiles of <jats:styled-content style=\"fixed-case\">MTC<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">GABA<\/jats:styled-content> pathways were analyzed. Subsequently, the <jats:styled-content style=\"fixed-case\">MTC<\/jats:styled-content>\u2010associated genes <jats:italic><jats:styled-content style=\"fixed-case\">SAMS<\/jats:styled-content>2<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">SAHH<\/jats:styled-content>1<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>1<\/jats:italic> and the <jats:styled-content style=\"fixed-case\">GABA<\/jats:styled-content> biosynthesis\u2010related genes <jats:italic><jats:styled-content style=\"fixed-case\">GAD<\/jats:styled-content>2<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">SSADH<\/jats:styled-content>1<\/jats:italic> were knocked\u2010down by virus\u2010induced gene silencing and the plants\u2019 defense responses upon infection with <jats:italic>R.\u00a0solanacearum<\/jats:italic> RsM and RsH were analyzed. These results showed that silencing of <jats:italic><jats:styled-content style=\"fixed-case\">SAHH<\/jats:styled-content>1<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>1<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">GAD<\/jats:styled-content>2<\/jats:italic> in tomato leads to decreased resistance against <jats:italic>R.\u00a0solanacearum<\/jats:italic>. In summary, the infection assays, proteomic and transcriptomic data described in this study indicate that both <jats:styled-content style=\"fixed-case\">MTC<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">GABA<\/jats:styled-content> biosynthesis play an important role in pathogenic interaction between <jats:italic>R.\u00a0solanacearum<\/jats:italic> and tomato plants.<\/jats:p>","DOI":"10.1111\/tpj.14175","type":"journal-article","created":{"date-parts":[[2018,11,27]],"date-time":"2018-11-27T13:38:39Z","timestamp":1543325919000},"page":"1032-1047","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":80,"title":["Resistance against <i>Ralstonia solanacearum<\/i> in tomato depends on the methionine cycle and the \u03b3\u2010aminobutyric acid metabolic pathway"],"prefix":"10.1111","volume":"97","author":[{"given":"Guoping","family":"Wang","sequence":"first","affiliation":[{"name":"College of Horticulture South 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