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Here, we employed RNA interference (RNAi), virus\u2010induced gene silencing (VIGS) technology, phytohormone profiling, genetic studies, and transcriptome and metabolome analysis to investigate the function of the tryptophan synthesis pathway in the resistance of cotton to <jats:italic>V.\u00a0dahliae<\/jats:italic>. We found that knock\u2010down of <jats:italic>GbTSA1<\/jats:italic> (Tryptophan Synthase \u03b1) and <jats:italic>GbTSB1<\/jats:italic> (tryptophan synthase \u03b2) induced a spontaneous cell death phenotype in a salicylic acid (SA)\u2010dependent manner and enhanced resistance to <jats:italic>V.\u00a0dahliae<\/jats:italic> in cotton plants. Metabolome analysis showed that indole and indolic metabolites were highly accumulated in <jats:italic>GbTSA1<\/jats:italic>\u2010 or <jats:italic>GbTSB1<\/jats:italic>\u2010silenced plants. Transcriptomic analysis showed that exogenous indole promotes the expression levels of genes involved in SA synthesis and the defense response. Similarly, indole application strongly enhanced cotton resistance to <jats:italic>V.\u00a0dahliae<\/jats:italic>. These results suggested that metabolic intermediates in the Trp synthesis pathway may be a signal to activate SA synthesis. These results also provided a strategy to elicit plant defense responses by the application of indole.<\/jats:p>","DOI":"10.1111\/tpj.14222","type":"journal-article","created":{"date-parts":[[2019,1,3]],"date-time":"2019-01-03T08:02:06Z","timestamp":1546502526000},"page":"329-345","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":72,"title":["Suppression of tryptophan synthase activates cotton immunity by triggering cell death via promoting <scp>SA<\/scp> synthesis"],"prefix":"10.1111","volume":"98","author":[{"given":"Yuhuan","family":"Miao","sequence":"first","affiliation":[{"name":"National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan Hubei 430070 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lian","family":"Xu","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan Hubei 430070 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"He","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan Hubei 430070 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan Hubei 430070 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Shaban","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan Hubei 430070 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianlong","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan Hubei 430070 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longfu","family":"Zhu","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan Hubei 430070 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,2,21]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2011.11.002"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1026561029533"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.006999"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-008-9435-0"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2006.02716.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1171661"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1163732"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.218202"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2015.00024"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81912-1"},{"key":"e_1_2_9_12_1","first-page":"7363","article-title":"Molecular research and genetic engineering of resistance to Verticillium wilt in cotton: a review","volume":"8","author":"Cai Y.","year":"2009","journal-title":"Afr. 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