{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T01:32:30Z","timestamp":1787707950892,"version":"build-2784847793"},"reference-count":60,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,2,28]],"date-time":"2019-02-28T00:00:00Z","timestamp":1551312000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31772239"],"award-info":[{"award-number":["31772239"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The hypersensitive\u2010induced reaction (<jats:italic><jats:styled-content style=\"fixed-case\">HIR<\/jats:styled-content><\/jats:italic>) gene family is associated with the hypersensitive response (<jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content>) that is a part of the plant defense system against bacterial and fungal pathogens. The involvement of <jats:italic><jats:styled-content style=\"fixed-case\">HIR<\/jats:styled-content><\/jats:italic> genes in response to viral pathogens has not yet been studied. We now report that the <jats:italic><jats:styled-content style=\"fixed-case\">HIR<\/jats:styled-content>3<\/jats:italic> genes of <jats:italic>Nicotiana benthamiana<\/jats:italic> and <jats:italic>Oryza sativa<\/jats:italic> (rice) were upregulated following rice stripe virus (<jats:styled-content style=\"fixed-case\">RSV<\/jats:styled-content>) infection. Silencing of <jats:italic><jats:styled-content style=\"fixed-case\">HIR<\/jats:styled-content>3<\/jats:italic>s in <jats:italic>N.\u00a0benthamiana<\/jats:italic> resulted in an increased accumulation of <jats:styled-content style=\"fixed-case\">RSV RNA<\/jats:styled-content>s, whereas overexpression of <jats:italic><jats:styled-content style=\"fixed-case\">HIR<\/jats:styled-content>3s<\/jats:italic> in <jats:italic>N.\u00a0benthamiana<\/jats:italic> or rice reduced the expression of <jats:styled-content style=\"fixed-case\">RSV RNA<\/jats:styled-content>s and decreased symptom severity, while also conferring resistance to <jats:italic>Turnip mosaic virus<\/jats:italic>,<jats:italic> Potato virus X<\/jats:italic>, and the bacterial pathogens <jats:italic>Pseudomonas syringae<\/jats:italic> and <jats:italic>Xanthomonas oryzae<\/jats:italic>. Silencing of <jats:italic><jats:styled-content style=\"fixed-case\">HIR<\/jats:styled-content>3<\/jats:italic> genes in <jats:italic>N.\u00a0benthamiana<\/jats:italic> reduced the content of salicylic acid (<jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content>) and was accompanied by the downregulated expression of genes in the <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content> pathway. Transient expression of the two <jats:italic><jats:styled-content style=\"fixed-case\">HIR<\/jats:styled-content>3<\/jats:italic> gene homologs from <jats:italic>N.\u00a0benthamiana<\/jats:italic> or the rice <jats:italic><jats:styled-content style=\"fixed-case\">HIR<\/jats:styled-content>3<\/jats:italic> gene in <jats:italic>N.\u00a0benthamiana<\/jats:italic> leaves caused cell death and an accumulation of <jats:styled-content style=\"fixed-case\">SA,<\/jats:styled-content> but did not do so in <jats:italic><jats:styled-content style=\"fixed-case\">EDS<\/jats:styled-content>1<\/jats:italic>\u2010silenced plants or in plants expressing <jats:italic>NahG<\/jats:italic>. The results indicate that <jats:italic><jats:styled-content style=\"fixed-case\">HIR<\/jats:styled-content>3<\/jats:italic> contributes to plant basal resistance via an <jats:italic><jats:styled-content style=\"fixed-case\">EDS<\/jats:styled-content>1<\/jats:italic>\u2010 and SA\u2010dependent pathway.<\/jats:p>","DOI":"10.1111\/tpj.14271","type":"journal-article","created":{"date-parts":[[2019,2,7]],"date-time":"2019-02-07T14:21:10Z","timestamp":1549549270000},"page":"783-797","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":81,"title":["The <i>hypersensitive induced reaction 3<\/i> (<i><scp>HIR<\/scp>3<\/i>) gene contributes to plant basal resistance via an <i><scp>EDS<\/scp>1<\/i> and salicylic acid\u2010dependent pathway"],"prefix":"10.1111","volume":"98","author":[{"given":"Saisai","family":"Li","sequence":"first","affiliation":[{"name":"College of Agriculture and Biotechnology Zhejiang University Hangzhou 310058 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