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Volatiles play an important role in the interaction of a plant with its environment. Through transcript profiling of jasmonate\u2010elicited <jats:italic>Medicago truncatula<\/jats:italic> cells, we identified Emission of Methyl Anthranilate (<jats:styled-content style=\"fixed-case\">EMA<\/jats:styled-content>) 1, a <jats:styled-content style=\"fixed-case\">MYB<\/jats:styled-content> transcription factor that is involved in the emission of the volatile compound methyl anthranilate when expressed in <jats:italic>M.\u00a0truncatula<\/jats:italic> hairy roots, giving them a fruity scent. <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> sequencing (<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010Seq) analysis of the fragrant roots revealed the upregulation of a methyltransferase that was subsequently characterized to catalyze the <jats:italic>O<\/jats:italic>\u2010methylation of anthranilic acid and was hence named <jats:italic>M.\u00a0truncatula<\/jats:italic> anthranilic acid methyl transferase (Mt<jats:styled-content style=\"fixed-case\">AAMT<\/jats:styled-content>) 1. Given that direct activation of the <jats:italic>Mt<jats:styled-content style=\"fixed-case\">AAMT<\/jats:styled-content>1<\/jats:italic> promoter by <jats:styled-content style=\"fixed-case\">EMA<\/jats:styled-content>1 could not be unambiguously demonstrated, we further probed the <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010Seq data and identified the repressor protein <jats:italic>M.\u00a0truncatula<\/jats:italic> plant <jats:styled-content style=\"fixed-case\">AT<\/jats:styled-content>\u2010rich sequence and zinc\u2010binding (Mt<jats:styled-content style=\"fixed-case\">PLATZ<\/jats:styled-content>) 1. Emission of Methyl Anthranilate 1 binds a tandem repeat of the <jats:styled-content style=\"fixed-case\">ACCTAAC<\/jats:styled-content> motif in the Mt<jats:styled-content style=\"fixed-case\">PLATZ<\/jats:styled-content>1 promoter to transactivate gene expression. Overexpression of <jats:italic>Mt<jats:styled-content style=\"fixed-case\">PLATZ<\/jats:styled-content>1<\/jats:italic> in transgenic <jats:italic>M.\u00a0truncatula<\/jats:italic> hairy roots led to transcriptional silencing of <jats:italic><jats:styled-content style=\"fixed-case\">EMA<\/jats:styled-content>1<\/jats:italic>, indicating that Mt<jats:styled-content style=\"fixed-case\">PLATZ<\/jats:styled-content>1 may be part of a negative feedback loop to control the expression of <jats:italic><jats:styled-content style=\"fixed-case\">EMA<\/jats:styled-content>1<\/jats:italic>. Finally, application of exogenous methyl anthranilate boosted <jats:italic><jats:styled-content style=\"fixed-case\">EMA<\/jats:styled-content>1<\/jats:italic> and <jats:italic>Mt<jats:styled-content style=\"fixed-case\">AAMT<\/jats:styled-content>1<\/jats:italic> expression dramatically, thus also revealing a positive amplification loop. Such positive and negative feedback loops seem to be the norm rather than the exception in the regulation of plant specialized metabolism.<\/jats:p>","DOI":"10.1111\/tpj.14347","type":"journal-article","created":{"date-parts":[[2019,4,22]],"date-time":"2019-04-22T14:10:04Z","timestamp":1555942204000},"page":"637-654","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["The <scp>MYB<\/scp> transcription factor Emission of Methyl Anthranilate 1 stimulates emission of methyl anthranilate from <i>Medicago truncatula<\/i> hairy roots"],"prefix":"10.1111","volume":"99","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1134-9238","authenticated-orcid":false,"given":"Jacob","family":"Pollier","sequence":"first","affiliation":[{"name":"Department of Plant Biotechnology and Bioinformatics Ghent University Technologiepark 71 B\u20109052 Ghent Belgium"},{"name":"VIB Center for Plant Systems Biology Technologiepark 71 B\u20109052 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