{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T05:04:59Z","timestamp":1792386299631,"version":"4.4.0"},"reference-count":44,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T00:00:00Z","timestamp":1567987200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001868","name":"National Science Council","doi-asserted-by":"publisher","award":["MOST106\u20102311\u2010B\u2010038\u2010002"],"award-info":[{"award-number":["MOST106\u20102311\u2010B\u2010038\u2010002"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001868","name":"National Science Council","doi-asserted-by":"publisher","award":["MOST107\u20102313\u2010B\u2010002\u2010039\u2010MY3"],"award-info":[{"award-number":["MOST107\u20102313\u2010B\u2010002\u2010039\u2010MY3"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001868","name":"National Science Council","doi-asserted-by":"publisher","award":["MOST107\u20102313\u2010B\u2010038\u2010001"],"award-info":[{"award-number":["MOST107\u20102313\u2010B\u2010038\u2010001"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001868","name":"National Science Council","doi-asserted-by":"publisher","award":["NSC103\u20102311\u2010B\u2010038\u2010005\u2010MY3"],"award-info":[{"award-number":["NSC103\u20102311\u2010B\u2010038\u2010005\u2010MY3"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Phytoplasmas are bacterial plant pathogens which can induce severe symptoms including dwarfism, phyllody and virescence in an infected plant. Because phytoplasmas infect many important crops such as peanut and papaya they have caused serious agricultural losses. The phytoplasmal effector causing phyllody 1 (<jats:styled-content style=\"fixed-case\">PHYL<\/jats:styled-content>1) is an important phytoplasmal pathogenic factor which affects the biological function of <jats:styled-content style=\"fixed-case\">MADS<\/jats:styled-content> transcription factors by interacting with their K (keratin\u2010like) domain, thus resulting in abnormal plant developments such as phyllody. Until now, lack of information on the structure of <jats:styled-content style=\"fixed-case\">PHYL<\/jats:styled-content>1 has prevented a detailed understanding of the binding mechanism between <jats:styled-content style=\"fixed-case\">PHYL<\/jats:styled-content>1 and the <jats:styled-content style=\"fixed-case\">MADS<\/jats:styled-content> transcription factors. Here, we present the crystal structure of <jats:styled-content style=\"fixed-case\">PHYL<\/jats:styled-content>1 from peanut witches\u2019\u2010broom phytoplasma (<jats:styled-content style=\"fixed-case\">PHYL<\/jats:styled-content>1<jats:sub>Pn<\/jats:sub><jats:styled-content style=\"fixed-case\"><jats:sub>WB<\/jats:sub><\/jats:styled-content>). This protein was found to fold into a unique \u03b1\u2010helical hairpin with exposed hydrophobic residues on its surface that may play an important role in its biological function. Using proteomics approaches, we propose a binding mode of <jats:styled-content style=\"fixed-case\">PHYL<\/jats:styled-content>1<jats:sub>Pn<\/jats:sub><jats:styled-content style=\"fixed-case\"><jats:sub>WB<\/jats:sub><\/jats:styled-content> with the K domain of the <jats:styled-content style=\"fixed-case\">MADS<\/jats:styled-content> transcription factor <jats:styled-content style=\"fixed-case\">SEPALLATA<\/jats:styled-content>3 (<jats:styled-content style=\"fixed-case\">SEP<\/jats:styled-content>3_K) and identify the residues of <jats:styled-content style=\"fixed-case\">PHYL<\/jats:styled-content>1<jats:sub>Pn<\/jats:sub><jats:styled-content style=\"fixed-case\"><jats:sub>WB<\/jats:sub><\/jats:styled-content> that are important for this interaction. Furthermore, using surface plasmon resonance we measure the binding strength of <jats:styled-content style=\"fixed-case\">PHYL<\/jats:styled-content>1<jats:sub>Pn<\/jats:sub><jats:styled-content style=\"fixed-case\"><jats:sub>WB<\/jats:sub><\/jats:styled-content> proteins to <jats:styled-content style=\"fixed-case\">SEP<\/jats:styled-content>3_K. Lastly, based on confocal images, we found that \u03b1\u2010helix 2 of <jats:styled-content style=\"fixed-case\">PHYL<\/jats:styled-content>1<jats:sub>Pn<\/jats:sub><jats:styled-content style=\"fixed-case\"><jats:sub>WB<\/jats:sub><\/jats:styled-content> plays an important role in <jats:styled-content style=\"fixed-case\">PHYL<\/jats:styled-content>1\u2010mediated degradation of <jats:styled-content style=\"fixed-case\">SEP<\/jats:styled-content>3. Taken together, these results provide a structural understanding of the specific binding mechanism between <jats:styled-content style=\"fixed-case\">PHYL<\/jats:styled-content>1<jats:sub>Pn<\/jats:sub><jats:styled-content style=\"fixed-case\"><jats:sub>WB<\/jats:sub><\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">SEP<\/jats:styled-content>3_K.<\/jats:p>","DOI":"10.1111\/tpj.14463","type":"journal-article","created":{"date-parts":[[2019,7,19]],"date-time":"2019-07-19T16:18:07Z","timestamp":1563553087000},"page":"706-719","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Structural insights into the interaction between phytoplasmal effector causing phyllody 1 and <scp>MADS<\/scp> transcription factors"],"prefix":"10.1111","volume":"100","author":[{"given":"Yi\u2010Ting","family":"Liao","sequence":"first","affiliation":[{"name":"The PhD Program for Translational Medicine College of Medical Science and Technology Taipei Medical University and Academia Sinica Taipei 11529 Taiwan"},{"name":"Graduate Institute of Translational Medicine College of Medical Science and Technology Taipei Medical University Taipei 11031 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shih\u2010Shun","family":"Lin","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology National Taiwan University Taipei 10617 Taiwan"},{"name":"Agricultural Biotechnology Research Center Academia Sinica Taipei 11529 Taiwan"},{"name":"Center of Biotechnology National Taiwan University Taipei 10617 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shin\u2010Jen","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Bioindustry Sciences College of Bioscience and Biotechnology National Cheng Kung University Tainan 70101 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wan\u2010Ting","family":"Sun","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology National Taiwan University Taipei 10617 Taiwan"},{"name":"Department of Plant Pathology and Microbiology College of Bioresources and Agriculture National Taiwan University Taipei 10617 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing\u2010Nan","family":"Shen","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology National Taiwan University Taipei 10617 Taiwan"},{"name":"Department of Plant Pathology and Microbiology College of Bioresources and Agriculture National Taiwan University Taipei 10617 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han\u2010Pin","family":"Cheng","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology National Taiwan University Taipei 10617 Taiwan"},{"name":"Department of Plant Pathology and Microbiology College of Bioresources and Agriculture National Taiwan University Taipei 10617 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chan\u2010Pin","family":"Lin","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology National Taiwan University Taipei 10617 Taiwan"},{"name":"Department of Plant Pathology and Microbiology College of Bioresources and Agriculture National Taiwan University Taipei 10617 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tzu\u2010Ping","family":"Ko","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry Academia Sinica Taipei 11529 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Fan","family":"Chen","sequence":"additional","affiliation":[{"name":"The PhD Program for Translational Medicine College of Medical Science and Technology Taipei Medical University and Academia Sinica Taipei 11529 Taiwan"},{"name":"Graduate Institute of Translational Medicine College of Medical Science and Technology Taipei Medical University Taipei 11031 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2396-717X","authenticated-orcid":false,"given":"Hao\u2010Ching","family":"Wang","sequence":"additional","affiliation":[{"name":"The PhD Program for Translational Medicine College of Medical Science and Technology Taipei Medical University and Academia Sinica Taipei 11529 Taiwan"},{"name":"Graduate Institute of Translational Medicine College of Medical Science and Technology Taipei Medical University Taipei 11031 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