{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T01:01:42Z","timestamp":1789520502048,"version":"build-2803163510"},"reference-count":100,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,9,24]],"date-time":"2019-09-24T00:00:00Z","timestamp":1569283200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010663","name":"H2020 European Research Council","doi-asserted-by":"publisher","award":["679814"],"award-info":[{"award-number":["679814"]}],"id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Chlorella vulgaris<\/jats:italic>\n                    is a fast\u2010growing fresh\u2010water microalga cultivated on the industrial scale for applications ranging from food to biofuel production. To advance our understanding of its biology and to establish genetics tools for biotechnological manipulation, we sequenced the nuclear and organelle genomes of\n                    <jats:italic>Chlorella vulgaris<\/jats:italic>\n                    211\/11P by combining next generation sequencing and optical mapping of isolated DNA molecules. This hybrid approach allowed us to assemble the nuclear genome in 14 pseudo\u2010molecules with an N50 of 2.8\u00a0Mb and 98.9% of scaffolded genome. The integration of RNA\u2010seq data obtained at two different irradiances of growth (high light, HL versus low light, LL) enabled us to identify 10\u00a0724 nuclear genes, coding for 11\u00a0082 transcripts. Moreover, 121 and 48 genes, respectively, were found in the chloroplast and mitochondrial genome. Functional annotation and expression analysis of nuclear, chloroplast and mitochondrial genome sequences revealed particular features of\n                    <jats:italic>Chlorella vulgaris<\/jats:italic>\n                    . Evidence of horizontal gene transfers from chloroplast to mitochondrial genome was observed. Furthermore, comparative transcriptomic analyses of LL versus HL provided insights into the molecular basis for metabolic rearrangement under HL versus LL conditions leading to enhanced\n                    <jats:italic>de novo<\/jats:italic>\n                    fatty acid biosynthesis and triacylglycerol accumulation. The occurrence of a cytosolic fatty acid biosynthetic pathway could be predicted and its upregulation upon HL exposure was observed, consistent with the increased lipid amount under HL conditions. These data provide a rich genetic resource for future genome editing studies, and potential targets for biotechnological manipulation of\n                    <jats:italic>Chlorella vulgaris<\/jats:italic>\n                    or other microalgae species to improve biomass and lipid productivity.\n                  <\/jats:p>","DOI":"10.1111\/tpj.14508","type":"journal-article","created":{"date-parts":[[2019,8,22]],"date-time":"2019-08-22T16:40:51Z","timestamp":1566492051000},"page":"1289-1305","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":90,"title":["<i>Chlorella vulgaris<\/i>\n                    genome assembly and annotation reveals the molecular basis for metabolic acclimation to high light conditions"],"prefix":"10.1111","volume":"100","author":[{"given":"Michela","family":"Cecchin","sequence":"first","affiliation":[{"name":"Dipartimento di Biotecnologie Universit\u00e0 di Verona Strada Le Grazie 15 37134 Verona,  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