{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T15:09:41Z","timestamp":1791472181831,"version":"4.3.4"},"reference-count":80,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,10,22]],"date-time":"2019-10-22T00:00:00Z","timestamp":1571702400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31700187"],"award-info":[{"award-number":["31700187"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    CG methylation (\n                    <jats:sup>m<\/jats:sup>\n                    CG) is essential for preserving genome stability in mammals, but this link remains obscure in plants. OsMET1\u20102, a major rice DNA methyltransferase, plays critical roles in maintaining\n                    <jats:sup>m<\/jats:sup>\n                    CG in rice. Null mutation of\n                    <jats:italic>OsMET1\u20102<\/jats:italic>\n                    causes massive CG hypomethylation, rendering the mutant suitable to address the role of\n                    <jats:sup>m<\/jats:sup>\n                    CG in maintaining genome integrity in plants. Here, we analyzed\n                    <jats:sup>m<\/jats:sup>\n                    CG dynamics and genome stability in tissue cultures of\n                    <jats:italic>OsMET1\u20102<\/jats:italic>\n                    homozygous (\u2212\/\u2212) and heterozygous (+\/\u2212) mutants, and isogenic wild\u2010type (WT). We found\n                    <jats:sup>m<\/jats:sup>\n                    CG levels in cultures of \u2212\/\u2212 were substantially lower than in those of WT and +\/\u2212, as expected. Unexpectedly,\n                    <jats:sup>m<\/jats:sup>\n                    CG levels in 1\u2010 and 3\u2010year cultures of \u2212\/\u2212 were 77.6% and 48.7% higher, respectively, than in shoot, from which the cultures were initiated, suggesting substantial regain of\n                    <jats:sup>m<\/jats:sup>\n                    CG in \u2212\/\u2212 cultures, which contrasts to the general trend of\n                    <jats:sup>m<\/jats:sup>\n                    CG loss in all WT plant tissue cultures hitherto studied. Transpositional burst of diverse transposable elements (TEs) occurred only in \u2212\/\u2212 cultures, although no elevation of genome\u2010wide mutation rate in the form of single nucleotide polymorphisms was detected. Altogether, our results establish an essential role of\n                    <jats:sup>m<\/jats:sup>\n                    CG in retaining TE immobility and hence genome stability in rice and likely in plants in general.\n                  <\/jats:p>","DOI":"10.1111\/tpj.14531","type":"journal-article","created":{"date-parts":[[2019,9,17]],"date-time":"2019-09-17T03:03:31Z","timestamp":1568689411000},"page":"188-203","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["CG hypomethylation leads to complex changes in\n                    <scp>DNA<\/scp>\n                    methylation and transpositional burst of diverse transposable elements in callus cultures of rice"],"prefix":"10.1111","volume":"101","author":[{"given":"Lanjuan","family":"Hu","sequence":"first","affiliation":[{"name":"Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE) Northeast Normal University Changchun 130024 China"},{"name":"College of Plant Sciences Faculty of Agriculture Jilin University Changchun 130062 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7721-8222","authenticated-orcid":false,"given":"Ning","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE) Northeast Normal University Changchun 130024 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhibin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE) Northeast Normal University Changchun 130024 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinchao","family":"Meng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE) Northeast Normal University Changchun 130024 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