{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T02:34:50Z","timestamp":1789180490970,"version":"build-2803163510"},"reference-count":83,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,12,8]],"date-time":"2019-12-08T00:00:00Z","timestamp":1575763200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005908","name":"Bundesministerium f\u00fcr Ern\u00e4hrung und Landwirtschaft","doi-asserted-by":"publisher","award":["2811NA09"],"award-info":[{"award-number":["2811NA09"]}],"id":[{"id":"10.13039\/501100005908","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Bunch rot caused by <jats:italic>Botrytis cinerea<\/jats:italic> infections is a notorious problem in grapevine cultivation. To produce high quality fruits, grapevine plants are treated with fungicides, which is cost intensive and harmful to the environment. Conversely, loose cluster bunches show a considerably enhanced physical resilience to bunch diseases. With the aim to identify genetic determinants that modulate the development of bunch architecture, we have compared loose and compact 'Pinot noir' clones. Loose cluster architecture was found to be correlated with increased berry size, elongated rachis and elongated pedicels. Using transcriptome analysis in combination with whole genome sequencing, we have identified a growth\u2010regulating factor gene, <jats:italic>VvGRF4<\/jats:italic>, upregulated and harbours heterozygous mutations in the loose cluster clones. At late stages of inflorescence development, the mRNA pools of loose cluster clones contain predominantly mRNAs derived from the mutated alleles, which are resistant to miR396 degradation. Expression of the <jats:italic>VvGRF4<\/jats:italic> gene and its mutated variants in Arabidopsis demonstrates that it promotes pedicel elongation. Taken together, VvGRF4 modulates bunch architecture in grapevine 'Pinot noir' clones. This trait can be introduced into other cultivars using marker\u2010assisted breeding or CRISPR\u2010Cas9 technology. Related growth\u2010regulating factors or other genes of the same pathway may have similar functions.<\/jats:p>","DOI":"10.1111\/tpj.14588","type":"journal-article","created":{"date-parts":[[2019,10,30]],"date-time":"2019-10-30T11:58:09Z","timestamp":1572436689000},"page":"1234-1248","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Mutations in the miR396 binding site of the growth\u2010regulating factor gene <scp><i>VvGRF4<\/i><\/scp> modulate inflorescence architecture in grapevine"],"prefix":"10.1111","volume":"101","author":[{"given":"Susanne","family":"Rossmann","sequence":"first","affiliation":[{"name":"Max Planck Institute for Plant Breeding Research 50931 Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Richter","sequence":"additional","affiliation":[{"name":"Federal Research Centre for Cultivated Plants Institute for Grapevine Breeding Geilweilerhof Julius\u2010Kuehn Institute 76833 Siebeldingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hequan","family":"Sun","sequence":"additional","affiliation":[{"name":"Max Planck Institute for Plant Breeding Research 50931 Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Korbinian","family":"Schneeberger","sequence":"additional","affiliation":[{"name":"Max Planck Institute for Plant Breeding Research 50931 Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reinhard","family":"T\u00f6pfer","sequence":"additional","affiliation":[{"name":"Federal Research Centre for Cultivated Plants Institute for Grapevine Breeding Geilweilerhof Julius\u2010Kuehn Institute 76833 Siebeldingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Zyprian","sequence":"additional","affiliation":[{"name":"Federal Research Centre for Cultivated Plants Institute for Grapevine Breeding Geilweilerhof Julius\u2010Kuehn Institute 76833 Siebeldingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6387-2517","authenticated-orcid":false,"given":"Klaus","family":"Theres","sequence":"additional","affiliation":[{"name":"Max Planck Institute for Plant Breeding Research 50931 Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,12,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/15538360802597481"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btg173"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1438-8677.2012.00696.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.12178"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1746-4811-9-39"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-85-97"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2016.02.012"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.002428"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nplants.2015.195"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pch098"},{"key":"e_1_2_9_12_1","first-page":"301","article-title":"Relation of gibberellin treatment to fruit\u2010set, berry development, and cluster compactness in Vitis vinifera grapes","volume":"92","author":"Christodoulou A.J.","year":"1968","journal-title":"Proceedings of the American Society for Horticultural Science"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313x.1998.00343.x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.12567"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10658-009-9503-6"},{"key":"e_1_2_9_16_1","unstructured":"OIV. 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