{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:53:10Z","timestamp":1790740390789,"version":"4.1.0"},"reference-count":82,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,4,21]],"date-time":"2020-04-21T00:00:00Z","timestamp":1587427200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010663","name":"H2020 European Research Council","doi-asserted-by":"publisher","award":["336808"],"award-info":[{"award-number":["336808"]}],"id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    The broad host range necrotrophic fungus\n                    <jats:italic>Sclerotinia sclerotiorum<\/jats:italic>\n                    is a devastating pathogen of many oil and vegetable crops. Plant genes conferring complete resistance against\n                    <jats:italic>S.\u00a0sclerotiorum<\/jats:italic>\n                    have not been reported. Instead, plant populations challenged by\n                    <jats:italic>S.\u00a0sclerotiorum<\/jats:italic>\n                    exhibit a continuum of partial resistance designated as quantitative disease resistance (QDR). Because of their complex interplay and their small phenotypic effect, the functional characterization of QDR genes remains limited. How broad host range necrotrophic fungi manipulate plant programmed cell death is for instance largely unknown. Here, we designed a time\u2010resolved automated disease phenotyping pipeline enabling high\u2010throughput disease lesion measurement with high resolution, low footprint at low cost. We could accurately recover contrasted disease responses in several pathosystems using this system. We used our phenotyping pipeline to assess the kinetics of disease symptoms caused by seven\n                    <jats:italic>S.\u00a0sclerotiorum<\/jats:italic>\n                    isolates on six\n                    <jats:italic>A.\u00a0thaliana<\/jats:italic>\n                    natural accessions with unprecedented resolution. Large effect polymorphisms common to the most resistant\n                    <jats:italic>A.\u00a0thaliana<\/jats:italic>\n                    accessions identified highly divergent alleles of the nucleotide\u2010binding site leucine\u2010rich repeat gene\n                    <jats:italic>LAZ5<\/jats:italic>\n                    in the resistant accessions Rubezhnoe and Lip\u20100. We show that impaired\n                    <jats:italic>LAZ5<\/jats:italic>\n                    expression in\n                    <jats:italic>laz5.1<\/jats:italic>\n                    mutant lines and in\n                    <jats:italic>A.\u00a0thaliana<\/jats:italic>\n                    Rub natural accession correlate with enhanced QDR to\n                    <jats:italic>S.\u00a0sclerotiorum<\/jats:italic>\n                    . These findings illustrate the value of time\u2010resolved image\u2010based phenotyping for unravelling the genetic bases of complex traits such as QDR. Our results suggest that\n                    <jats:italic>S.\u00a0sclerotiorum<\/jats:italic>\n                    manipulates plant sphingolipid pathways guarded by LAZ5 to trigger programmed cell death and cause disease.\n                  <\/jats:p>","DOI":"10.1111\/tpj.14747","type":"journal-article","created":{"date-parts":[[2020,3,14]],"date-time":"2020-03-14T04:56:03Z","timestamp":1584161763000},"page":"903-917","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":63,"title":["Rapid identification of an Arabidopsis NLR gene as a candidate conferring susceptibility to\n                    <i>Sclerotinia sclerotiorum<\/i>\n                    using time\u2010resolved automated phenotyping"],"prefix":"10.1111","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3156-272X","authenticated-orcid":false,"given":"Adelin","family":"Barbacci","sequence":"first","affiliation":[{"name":"Laboratoire des Interactions Plantes Micro-organismes (LIPM) Universit\u00e9 de Toulouse INRAE CNRS 24 chemin de Borde Rouge - Auzeville CS 52627 F31326 Castanet Tolosan Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4270-4690","authenticated-orcid":false,"given":"Olivier","family":"Navaud","sequence":"additional","affiliation":[{"name":"Laboratoire des Interactions Plantes Micro-organismes (LIPM) Universit\u00e9 de Toulouse INRAE CNRS 24 chemin de Borde Rouge - Auzeville CS 52627 F31326 Castanet Tolosan Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7723-7757","authenticated-orcid":false,"given":"Malick","family":"Mbengue","sequence":"additional","affiliation":[{"name":"Laboratoire des Interactions Plantes Micro-organismes (LIPM) Universit\u00e9 de Toulouse INRAE CNRS 24 chemin de Borde Rouge - Auzeville CS 52627 F31326 Castanet Tolosan Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3753-2188","authenticated-orcid":false,"given":"Marielle","family":"Barascud","sequence":"additional","affiliation":[{"name":"Laboratoire des Interactions Plantes Micro-organismes (LIPM) Universit\u00e9 de Toulouse INRAE CNRS 24 chemin de Borde Rouge - Auzeville CS 52627 F31326 Castanet Tolosan Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5332-092X","authenticated-orcid":false,"given":"Laurence","family":"Godiard","sequence":"additional","affiliation":[{"name":"Laboratoire des Interactions Plantes Micro-organismes (LIPM) Universit\u00e9 de Toulouse INRAE CNRS 24 chemin de Borde Rouge - Auzeville CS 52627 F31326 Castanet Tolosan Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehdi","family":"Khafif","sequence":"additional","affiliation":[{"name":"Laboratoire des Interactions Plantes Micro-organismes (LIPM) Universit\u00e9 de Toulouse INRAE CNRS 24 chemin de Borde Rouge - Auzeville CS 52627 F31326 Castanet Tolosan Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline","family":"Lacaze","sequence":"additional","affiliation":[{"name":"Laboratoire des Interactions Plantes Micro-organismes (LIPM) Universit\u00e9 de Toulouse INRAE CNRS 24 chemin de Borde Rouge - Auzeville CS 52627 F31326 Castanet Tolosan Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2442-9632","authenticated-orcid":false,"given":"Sylvain","family":"Raffaele","sequence":"additional","affiliation":[{"name":"Laboratoire des Interactions Plantes Micro-organismes (LIPM) Universit\u00e9 de Toulouse INRAE CNRS 24 chemin de Borde Rouge - Auzeville CS 52627 F31326 Castanet Tolosan Cedex 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