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Seedling grafting in Arabidopsis, known as micrografting, enables investigation of the molecular mechanisms of systemic signaling between shoots and roots. However, conventional micrografting requires a high level of skill, limiting its use. Thus, an easier user\u2010friendly method is needed. Here, we developed a silicone microscaled device, the micrografting chip, to obviate the need for training and to generate less stressed and more uniformly grafted seedlings. The chip has tandemly arrayed units, each of which consists of a seed pocket for seed germination and a micro\u2010path with pairs of pillars for hypocotyl holding. Grafting, including seed germination, micrografting manipulation and establishment of tissue reunion, is performed on the chip. Using the micrografting chip, we evaluated the effect of temperature and the carbon source on grafting, and showed that a temperature of 27\u00b0C and a sucrose concentration of 0.5% were optimal. We also used the chip to investigate the mechanism of systemic signaling of iron status using a quadruple nicotianamine synthase (<jats:italic>nas<\/jats:italic>) mutant. The constitutive iron\u2010deficiency response in the <jats:italic>nas<\/jats:italic> mutant because of iron accumulation in shoots was significantly rescued by grafting of wild\u2010type shoots or roots, suggesting that shoot\u2010 and root\u2010ward translocation of nicotianamine\u2013iron complexes and\/or nicotianamine is essential for iron mobilization. Thus, our micrografting chip will promote studies of long\u2010distance signaling in plants.<\/jats:p>","DOI":"10.1111\/tpj.14768","type":"journal-article","created":{"date-parts":[[2020,4,14]],"date-time":"2020-04-14T05:43:31Z","timestamp":1586843011000},"page":"918-929","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Micrografting device for testing systemic signaling in Arabidopsis"],"prefix":"10.1111","volume":"103","author":[{"given":"Hiroki","family":"Tsutsui","sequence":"first","affiliation":[{"name":"Graduate School of Bioagricultural Sciences Nagoya University Furo\u2010cho, Chikusa\u2010ku Nagoya 464\u20108601 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoki","family":"Yanagisawa","sequence":"additional","affiliation":[{"name":"Graduate School of Science Nagoya University Furo\u2010cho, Chikusa\u2010ku Nagoya 464\u20108601 Japan"},{"name":"Institute of Transformative Bio\u2010Molecules Nagoya University Nagoya 464\u20108601 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaichi","family":"Kawakatsu","sequence":"additional","affiliation":[{"name":"Graduate School of Bioagricultural Sciences Nagoya University Furo\u2010cho, Chikusa\u2010ku Nagoya 464\u20108601 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuka","family":"Ikematsu","sequence":"additional","affiliation":[{"name":"Graduate School of Science Nagoya University Furo\u2010cho, Chikusa\u2010ku Nagoya 464\u20108601 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Sawai","sequence":"additional","affiliation":[{"name":"Graduate School of Bioagricultural Sciences Nagoya University Furo\u2010cho, Chikusa\u2010ku Nagoya 464\u20108601 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryo","family":"Tabata","sequence":"additional","affiliation":[{"name":"Graduate School of Bioagricultural Sciences Nagoya University Furo\u2010cho, Chikusa\u2010ku Nagoya 464\u20108601 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideyuki","family":"Arata","sequence":"additional","affiliation":[{"name":"Graduate School of Science Nagoya University Furo\u2010cho, Chikusa\u2010ku Nagoya 464\u20108601 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuya","family":"Higashiyama","sequence":"additional","affiliation":[{"name":"Graduate School of Science Nagoya University Furo\u2010cho, Chikusa\u2010ku Nagoya 464\u20108601 Japan"},{"name":"Institute of Transformative Bio\u2010Molecules Nagoya University Nagoya 464\u20108601 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0831-2108","authenticated-orcid":false,"given":"Michitaka","family":"Notaguchi","sequence":"additional","affiliation":[{"name":"Graduate School of Bioagricultural Sciences Nagoya University Furo\u2010cho, Chikusa\u2010ku Nagoya 464\u20108601 Japan"},{"name":"Graduate School of Science Nagoya University Furo\u2010cho, Chikusa\u2010ku Nagoya 464\u20108601 Japan"},{"name":"Institute of Transformative Bio\u2010Molecules Nagoya University Nagoya 464\u20108601 Japan"},{"name":"Bioscience and Biotechnology Center Nagoya University Furo\u2010cho, Chikusa\u2010ku Nagoya 464\u20108601 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,4,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1141752"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2004.02128.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2016.00381"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.107.097162"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.111.093179"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1253736"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2015.00716"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.12.7197"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.111.092577"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2009.05.006"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13007-015-0081-7"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2011.02.020"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2017.06.014"},{"key":"e_1_2_9_15_1","doi-asserted-by":"crossref","unstructured":"Jin P. 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