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In <jats:italic>Arabidopsis thaliana<\/jats:italic>,<jats:italic> HPAT1<\/jats:italic> and <jats:italic>HPAT3<\/jats:italic> are redundantly required for full pollen fertility. In addition to the lack of Hyp\u2010Ara in <jats:italic>hpat1\/3<\/jats:italic> pollen tubes (PTs), we also found broadly disrupted cell wall polymer distributions, particularly the conversion of the tip cell wall to a more shaft\u2010like state. Mutant PTs were slow growing and prone to rupture and morphological irregularities. In a forward mutagenesis screen for suppressors of the <jats:italic>hpat1\/3<\/jats:italic> low seed\u2010set phenotype, we identified a missense mutation in <jats:italic>exo70a2<\/jats:italic>, a predicted member of the vesicle\u2010tethering exocyst complex. The suppressed pollen had increased fertility, fewer morphological defects and partially rescued cell wall organization. A transcriptional null allele of <jats:italic>exo70a2<\/jats:italic> also suppressed the <jats:italic>hpat1\/3<\/jats:italic> fertility phenotype, as did mutants of core exocyst complex member <jats:italic>sec15a<\/jats:italic>, indicating that reduced exocyst function bypassed the PT requirement for Hyp\u2010Ara. In a wild\u2010type background, <jats:italic>exo70a2<\/jats:italic> reduced male transmission efficiency, lowered pollen germination frequency and slowed PT elongation. EXO70A2 also localized to the PT tip plasma membrane, consistent with a role in exocyst\u2010mediated secretion. To monitor the trafficking of Hyp\u2010Ara modified proteins, we generated an HPAT\u2010targeted fluorescent secretion reporter. Reporter secretion was partially dependent on <jats:italic>EXO70A2<\/jats:italic> and was significantly increased in <jats:italic>hpat1\/3<\/jats:italic> PTs compared with the wild type, but was reduced in the suppressed <jats:italic>exo70a2\u00a0hpat1\/3<\/jats:italic> tubes.<\/jats:p>","DOI":"10.1111\/tpj.14808","type":"journal-article","created":{"date-parts":[[2020,5,11]],"date-time":"2020-05-11T10:13:05Z","timestamp":1589191985000},"page":"1399-1419","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Exocyst mutants suppress pollen tube growth and cell wall structural defects of <i>hydroxyproline O\u2010arabinosyltransferase<\/i> mutants"],"prefix":"10.1111","volume":"103","author":[{"given":"Steven","family":"Beuder","sequence":"first","affiliation":[{"name":"Department of Molecular, Cellular and Developmental Biology University of Michigan 1105 N. University Ave Ann Arbor MI 48109 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandria","family":"Dorchak","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular and Developmental Biology University of Michigan 1105 N. University Ave Ann Arbor MI 48109 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashwini","family":"Bhide","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular and Developmental Biology University of Michigan 1105 N. University Ave Ann Arbor MI 48109 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svenning Rune","family":"Moeller","sequence":"additional","affiliation":[{"name":"Department of Plant and Environmental Sciences Faculty of Science University of Copenhagen Thorvaldsensvej 40 K\u00f8benhavn 1871 Frederiksberg C Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bent L.","family":"Petersen","sequence":"additional","affiliation":[{"name":"Department of Plant and Environmental Sciences Faculty of Science University of Copenhagen Thorvaldsensvej 40 K\u00f8benhavn 1871 Frederiksberg C Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1470-0596","authenticated-orcid":false,"given":"Cora A.","family":"MacAlister","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular and Developmental Biology University of Michigan 1105 N. 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