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We studied the immunity network response conditioned by the potato\n                    <jats:italic>Ny\u20101<\/jats:italic>\n                    gene against potato virus Y. We analyzed the processes in the cell death zone and surrounding tissue on the biochemical and gene expression levels in order to reveal the spatiotemporal regulation of the immune response. We show that the transcriptional response in the cell death zone and surrounding tissue is dependent on salicylic acid (SA). For some genes the spatiotemporal regulation is completely lost in the SA\u2010deficient line, whereas other genes show a different response, indicating multiple connections between hormonal signaling modules. The induction of NADPH oxidase\n                    <jats:italic>RBOHD<\/jats:italic>\n                    expression occurs specifically on the lesion border during the resistance response. In plants with silenced\n                    <jats:italic>RBOHD<\/jats:italic>\n                    , the functionality of the resistance response is perturbed and the spread of the virus is not arrested at the site of infection.\n                    <jats:italic>RBOHD<\/jats:italic>\n                    is required for the spatial accumulation of SA, and conversely\n                    <jats:italic>RBOHD<\/jats:italic>\n                    is under the transcriptional regulation of SA. Using spatially resolved RNA\u2010seq, we also identified spatial regulation of an UDP\u2010glucosyltransferase, another component in feedback activation of SA biosynthesis, thus deciphering a novel aspect of resistance signaling.\n                  <\/jats:p>","DOI":"10.1111\/tpj.14953","type":"journal-article","created":{"date-parts":[[2020,8,9]],"date-time":"2020-08-09T16:47:15Z","timestamp":1596991635000},"page":"645-661","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":56,"title":["Precision transcriptomics of viral foci reveals the spatial regulation of immune\u2010signaling genes and identifies\n                    <i>RBOHD<\/i>\n                    as an important player in the incompatible interaction between potato virus Y and potato"],"prefix":"10.1111","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6235-2816","authenticated-orcid":false,"given":"Tja\u0161a","family":"Lukan","sequence":"first","affiliation":[{"name":"National Institute of Biology Ve\u010dna pot 111 Ljubljana 1000 Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maru\u0161a","family":"Pompe\u2010Novak","sequence":"additional","affiliation":[{"name":"National Institute of Biology Ve\u010dna pot 111 Ljubljana 1000 Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4776-7164","authenticated-orcid":false,"given":"\u0160pela","family":"Baebler","sequence":"additional","affiliation":[{"name":"National Institute of Biology Ve\u010dna pot 111 Ljubljana 1000 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