{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:22:02Z","timestamp":1787016122616,"version":"build-2736575974"},"reference-count":90,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,12,18]],"date-time":"2020-12-18T00:00:00Z","timestamp":1608249600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["INV\u2010007637"],"award-info":[{"award-number":["INV\u2010007637"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Manihot esculenta<\/jats:italic>\n                    (cassava) is a root crop originating from South America that is a major staple in the tropics, including in marginal environments. This study focused on South American and African germplasm and investigated the genetic architecture of hydrogen cyanide (HCN), a major component of root quality. HCN, representing total cyanogenic glucosides, is a plant defense component against herbivory but is also toxic for human consumption. We genotyped 3354 landraces and modern breeding lines originating from 26 Brazilian states and 1389 individuals were phenotypically characterized across multi\u2010year trials for HCN. All plant material was subjected to high\u2010density genotyping using genotyping by sequencing. We performed genome\u2010wide association mapping to characterize the genetic architecture and gene mapping of HCN. Field experiments revealed strong broad\u2010 and narrow\u2010sense trait heritability (0.82 and 0.41, respectively). Two major loci were identified, encoding for an ATPase and a MATE protein, and contributing up to 7 and 30% of the HCN concentration in roots, respectively. We developed diagnostic markers for breeding applications, validated trait architecture consistency in African germplasm and investigated further evidence for the domestication of sweet and bitter cassava. Fine genomic characterization revealed: (i) the major role played by vacuolar transporters in regulating HCN content; (ii) the co\u2010domestication of sweet and bitter cassava major alleles are dependent upon geographical zone; and (iii) the major loci allele for high HCN in\n                    <jats:italic>M.\u00a0esculenta<\/jats:italic>\n                    Crantz seems to originate from its ancestor,\n                    <jats:italic>M.\u00a0esculenta<\/jats:italic>\n                    subsp\n                    <jats:italic>. flabellifolia<\/jats:italic>\n                    . Taken together, these findings expand our insights into cyanogenic glucosides in cassava roots and its glycosylated derivatives in plants.\n                  <\/jats:p>","DOI":"10.1111\/tpj.15071","type":"journal-article","created":{"date-parts":[[2020,11,8]],"date-time":"2020-11-08T22:05:27Z","timestamp":1604873127000},"page":"754-770","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["Large\u2010scale genome\u2010wide association study, using historical data, identifies conserved genetic architecture of cyanogenic glucoside content in cassava (\n                    <i>Manihot esculenta<\/i>\n                    Crantz) root"],"prefix":"10.1111","volume":"105","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2407-0930","authenticated-orcid":false,"given":"Alex C.","family":"Ogbonna","sequence":"first","affiliation":[{"name":"Cornell University 135 Plant Science Building Ithaca NY 14850 USA"},{"name":"Boyce Thompson Institute 533 Tower Rd Ithaca NY 14853 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4752-1164","authenticated-orcid":false,"given":"Luciano Rogerio","family":"Braatz de Andrade","sequence":"additional","affiliation":[{"name":"Embrapa Mandioca e Fruticultura Rua Embrapa s\/n\u00ba, Caixa Postal 007 Cruz das Almas BA CEP: 44380\u2010000 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9966-2941","authenticated-orcid":false,"given":"Ismail Y.","family":"Rabbi","sequence":"additional","affiliation":[{"name":"International Institute of Tropical Agriculture PMB 5320, Oyo Road Ibadan Oyo State 200001 Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8640-1750","authenticated-orcid":false,"given":"Lukas A.","family":"Mueller","sequence":"additional","affiliation":[{"name":"Cornell University 135 Plant Science Building Ithaca NY 14850 USA"},{"name":"Boyce Thompson Institute 533 Tower Rd Ithaca NY 14853 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8992-7459","authenticated-orcid":false,"given":"Eder","family":"Jorge de Oliveira","sequence":"additional","affiliation":[{"name":"Embrapa Mandioca e Fruticultura Rua Embrapa s\/n\u00ba, Caixa Postal 007 Cruz das Almas BA CEP: 44380\u2010000 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3311-7726","authenticated-orcid":false,"given":"Guillaume J.","family":"Bauchet","sequence":"additional","affiliation":[{"name":"Boyce Thompson Institute 533 Tower Rd Ithaca NY 14853 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,12,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.114.173658"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12711-017-0348-8"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/aab.12460"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.3.1966"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.2135\/cropsci1968.0011183X000800010026x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v067.i01"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-7652.2011.00646.x"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.17660\/ActaHortic.1994.375.11"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0010(19990315)79:4<593::AID-JSFA222>3.0.CO;2-2"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3732\/ajb.1200482"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3535"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2009.01.005"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01162.x"},{"key":"e_1_2_9_15_1","first-page":"43","volume-title":"Chronic Cassava Toxicity: Proceedings of an Interdisciplinary Workshop, January 29\u201330, 1973, London","author":"Bruijn G.H.","year":"1973"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2229-4-10"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0118578"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.4161\/fly.19695"},{"key":"e_1_2_9_19_1","first-page":"178","article-title":"Cyanogenesis in white clover (Trifolium repens L.) 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