{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T14:26:52Z","timestamp":1780583212546,"version":"3.54.1"},"reference-count":64,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,2,19]],"date-time":"2021-02-19T00:00:00Z","timestamp":1613692800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004725","name":"Ministry of Economic Affairs","doi-asserted-by":"publisher","award":["KB15\u2010001\u2010007"],"award-info":[{"award-number":["KB15\u2010001\u2010007"]}],"id":[{"id":"10.13039\/501100004725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/P016855\/1"],"award-info":[{"award-number":["BB\/P016855\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Most alpha\u2010gliadin genes of the <jats:italic>Gli\u2010D2<\/jats:italic> locus on the D genome of hexaploid bread wheat (<jats:italic>Triticum aestivum<\/jats:italic>) encode for proteins with epitopes that can trigger coeliac disease (CD), and several contain a 33\u2010mer peptide with six partly overlapping copies of three epitopes, which is regarded as a remarkably potent T\u2010cell stimulator. To increase genetic diversity in the D genome, synthetic hexaploid wheat lines are being made by hybridising accessions of <jats:italic>Triticum turgidum<\/jats:italic> (AB genome) and <jats:italic>Aegilops tauschii<\/jats:italic> (the progenitor of the D genome). The diversity of alpha\u2010gliadins in <jats:italic>A. tauschii<\/jats:italic> has not been studied extensively. We analysed the alpha\u2010gliadin transcriptome of 51 <jats:italic>A. tauschii<\/jats:italic> accessions representative of the diversity in <jats:italic>A. tauschii<\/jats:italic>. We extracted RNA from developing seeds and performed 454 amplicon sequencing of the first part of the alpha\u2010gliadin genes. The expression profile of allelic variants of the alpha\u2010gliadins was different between accessions, and also between accessions of the Western and Eastern clades of <jats:italic>A. tauschii<\/jats:italic>. Generally, both clades expressed many allelic variants not found in bread wheat. In contrast to earlier studies, we detected the 33\u2010mer peptide in some <jats:italic>A. tauschii<\/jats:italic> accessions, indicating that it was introduced along with the D genome into bread wheat. In these accessions, transcripts with the 33\u2010mer peptide were present at lower frequencies than in bread wheat varieties. In most <jats:italic>A. tauschii<\/jats:italic> accessions, however, the alpha\u2010gliadins do not contain the epitope, and this may be exploited, through synthetic hexaploid wheats, to breed bread wheat varieties with fewer or no coeliac disease epitopes.<\/jats:p>","DOI":"10.1111\/tpj.15147","type":"journal-article","created":{"date-parts":[[2020,12,28]],"date-time":"2020-12-28T18:40:36Z","timestamp":1609180836000},"page":"86-94","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Exploring the alpha\u2010gliadin locus: the 33\u2010mer peptide with six overlapping coeliac disease epitopes in <i>Triticum aestivum<\/i> is derived from a subgroup of <i>Aegilops tauschii<\/i>"],"prefix":"10.1111","volume":"106","author":[{"given":"Jan G.","family":"Schaart","sequence":"first","affiliation":[{"name":"Plant Breeding Wageningen University and Research Droevendaalsesteeg 1 NL\u20106708 PB Wageningen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elma M. J.","family":"Salentijn","sequence":"additional","affiliation":[{"name":"Plant Breeding Wageningen University and Research Droevendaalsesteeg 1 NL\u20106708 PB Wageningen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svetlana V.","family":"Goryunova","sequence":"additional","affiliation":[{"name":"Plant Breeding Wageningen University and Research Droevendaalsesteeg 1 NL\u20106708 PB Wageningen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charity","family":"Chidzanga","sequence":"additional","affiliation":[{"name":"Plant Breeding Wageningen University and Research Droevendaalsesteeg 1 NL\u20106708 PB Wageningen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danny G.","family":"Esselink","sequence":"additional","affiliation":[{"name":"Plant Breeding Wageningen University and Research Droevendaalsesteeg 1 NL\u20106708 PB Wageningen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nick","family":"Gosman","sequence":"additional","affiliation":[{"name":"The John Bingham Laboratory NIAB 93 Lawrence Weaver Road Cambridge CB3 0LE UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alison R.","family":"Bentley","sequence":"additional","affiliation":[{"name":"The John Bingham Laboratory NIAB 93 Lawrence Weaver Road Cambridge CB3 0LE UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luud J. W. J.","family":"Gilissen","sequence":"additional","affiliation":[{"name":"Plant Breeding Wageningen University and Research Droevendaalsesteeg 1 NL\u20106708 PB Wageningen the Netherlands"},{"name":"Bioscience Wageningen University and Research Droevendaalsesteeg 1 NL\u20106708 PB Wageningen the Netherlands"},{"name":"Allergy Consortium Wageningen Droevendaalsesteeg 1 NL\u20106708 PB Wageningen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8549-6046","authenticated-orcid":false,"given":"Marinus J. M.","family":"Smulders","sequence":"additional","affiliation":[{"name":"Plant Breeding Wageningen University and Research Droevendaalsesteeg 1 NL\u20106708 PB Wageningen the Netherlands"},{"name":"Allergy Consortium Wageningen Droevendaalsesteeg 1 NL\u20106708 PB Wageningen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,2,19]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2005.064550"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-018-0007-9"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA118.005736"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10722-015-0312-9"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1143986"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10722-011-9711-8"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s001220050942"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2003.09.012"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.12259"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcs.2014.01.005"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10722-018-0711-9"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00122-019-03354-9"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.13675"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-23570-5"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10142-019-00686-z"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1601693"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00122-013-2093-x"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2017.04.025"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12870-019-1889-5"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcs.2019.102903"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2020.00051"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2019.00585"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10722-019-00758-w"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.2144\/05386BM05"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1139\/g91-057"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)13803-2"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365529609004874"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12862-015-0496-9"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0015637"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294X.2010.04537.x"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13353-020-00541-z"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2004.11.003"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00126756"},{"key":"e_1_2_9_35_1","unstructured":"Nesbitt M.andSamuel D.(1996)From staple crop to extinction? 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