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Members of the plant Skewed5 (SKU5) Similar (SKS) family show strong similarity to multicopper oxidases (MCOs), but they lack conserved histidines in MCO active sites. The functions of most SKS family members are unknown. Here, we show that Arabidopsis pollen\u2010expressed<jats:italic>SKS11<\/jats:italic>and<jats:italic>SKS12<\/jats:italic>play important roles in pollen tube integrity, growth and guidance. The<jats:italic>sks11sks12<\/jats:italic>mutant exhibited significantly reduced male fertility. Most of the pollen from<jats:italic>sks11sks12<\/jats:italic>plants burst when germinated, and the pollen tubes grew slowly and exhibited defective growth along the funiculus and micropyle. SKS11\u2010GFP and SKS12\u2010mCherry were detected at the cell wall in pollen tubes. The contents of several cell wall polysaccharides and arabinogalactans were decreased in the pollen tube cell walls of<jats:italic>sks11sks12<\/jats:italic>plants. Staining with a reactive oxygen species (ROS)\u2010sensitive dye and use of the H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>sensor HyPer revealed that the ROS content in the pollen tubes of<jats:italic>sks11sks12<\/jats:italic>plants was remarkably reduced. SKS11<jats:sup>444His\u2010Ala<\/jats:sup>, in which the last conserved histidine was mutated, could restore the mutant phenotypes of<jats:italic>sks11sks12<\/jats:italic>. Thus, SKS11\/12 are required for pollen tube integrity, growth and guidance possibly by regulating the ROS level and cell wall polysaccharide deposition or remodeling in pollen tubes.<\/jats:p>","DOI":"10.1111\/tpj.15580","type":"journal-article","created":{"date-parts":[[2021,11,14]],"date-time":"2021-11-14T15:17:30Z","timestamp":1636903050000},"page":"598-614","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Arabidopsis SKU5 Similar 11 and 12 play crucial roles in pollen tube integrity, growth and guidance"],"prefix":"10.1111","volume":"109","author":[{"given":"Yazhou","family":"Duan","sequence":"first","affiliation":[{"name":"Hebei Key Laboratory of Molecular and Cellular Biology College of Life Science Key Laboratory of Molecular and Cellular Biology of Ministry of Education Collaboration Innovation Center for Cell Signaling Hebei Normal University Shijia Zhuang Hebei 050024 P.R. 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