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Here we provide evidence supporting a second role of IRT1 in root\u2010to\u2010shoot partitioning of Fe. We show that <jats:italic>irt1<\/jats:italic> mutants overaccumulate Fe in roots, most prominently in the cortex of the differentiation zone in <jats:italic>irt1<\/jats:italic>\u20102, compared to the wild type. Shoots of <jats:italic>irt1<\/jats:italic>\u20102 are severely Fe\u2010deficient according to Fe content and marker transcripts, as expected. We generated <jats:italic>irt1<\/jats:italic>\u20102 lines producing IRT1 mutant variants carrying single amino\u2010acid substitutions of key residues in transmembrane helices IV and V, Ser206 and His232, which are required for transport activity in yeast. Root short\u2010term <jats:sup>55<\/jats:sup>Fe uptake rates were uninformative concerning IRT1\u2010mediated transport. Overall <jats:italic>irt1<\/jats:italic>\u2010like concentrations of the secondary substrate Mn suggested that the transgenic Arabidopsis lines also remain incapable of IRT1\u2010mediated root Fe uptake. Yet, IRT1<jats:sub>S206A<\/jats:sub> partially complements rosette dwarfing and leaf chlorosis of <jats:italic>irt1\u2010<\/jats:italic>2, as well as root\u2010to\u2010shoot Fe partitioning and gene expression defects of <jats:italic>irt1<\/jats:italic>\u20102, all of which are fully complemented by wild\u2010type IRT1. Taken together, these results suggest a regulatory function for IRT1 in root\u2010to\u2010shoot Fe partitioning that does not require Fe transport activity of IRT1. Among the genes of which transcript levels are partially dependent on IRT1, we identify <jats:italic>MYB DOMAIN PROTEIN10<\/jats:italic>, <jats:italic>MYB DOMAIN PROTEIN72<\/jats:italic> and <jats:italic>NICOTIANAMINE SYNTHASE4<\/jats:italic> as candidates for effecting IRT1\u2010dependent Fe mobilization in roots. Understanding the biological functions of IRT1 will help to improve Fe nutrition and the nutritional quality of agricultural crops.<\/jats:p>","DOI":"10.1111\/tpj.15611","type":"journal-article","created":{"date-parts":[[2021,11,28]],"date-time":"2021-11-28T14:07:06Z","timestamp":1638108426000},"page":"992-1013","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Root\u2010to\u2010shoot iron partitioning in Arabidopsis requires IRON\u2010REGULATED TRANSPORTER1 (IRT1) protein but not its iron(II) transport function"],"prefix":"10.1111","volume":"109","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5145-3590","authenticated-orcid":false,"given":"Julia","family":"Quintana","sequence":"first","affiliation":[{"name":"Faculty of Biology and Biotechnology Ruhr University Bochum 44801 Bochum 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