{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T02:32:58Z","timestamp":1784687578148,"version":"3.55.0"},"reference-count":67,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,4,6]],"date-time":"2022-04-06T00:00:00Z","timestamp":1649203200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2022,4,6]],"date-time":"2022-04-06T00:00:00Z","timestamp":1649203200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["IOS\u20101755482"],"award-info":[{"award-number":["IOS\u20101755482"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Pollen tubes (PTs) grow by the targeted secretion of new cell wall material to their expanding tip region. Sec1\/Munc18 (SM) proteins promote membrane fusion through regulation of the SNARE complex. We have previously shown that disruption of protein glycosylation in the <jats:italic>Arabidopsis thaliana hpat1 hpat3<\/jats:italic> double mutant leads to PT growth defects that can be suppressed by reducing secretion. Here, we identified five point mutant alleles of the SM protein <jats:italic>SEC1A<\/jats:italic> as <jats:italic>hpat1\/3<\/jats:italic> suppressors. The suppressors increased seed set, reduced PT growth defects and reduced the rate of glycoprotein secretion. In the absence of the <jats:italic>hpat<\/jats:italic> mutations, <jats:italic>sec1a<\/jats:italic> reduced pollen germination and PT elongation producing shorter and wider PTs. Consistent with a defect in membrane fusion, <jats:italic>sec1a<\/jats:italic> PTs accumulated secretory vesicles. Though <jats:italic>sec1a<\/jats:italic> had significantly reduced male transmission, homozygous <jats:italic>sec1a<\/jats:italic> plants maintained full seed set, demonstrating that <jats:italic>SEC1A<\/jats:italic> was ultimately dispensable for pollen fertility. However, when combined with a mutation in another <jats:italic>SEC1<\/jats:italic>\u2010like <jats:italic>SM<\/jats:italic> gene, <jats:italic>keule<\/jats:italic>, pollen fertility was totally abolished. Mutation in <jats:italic>sec1b<\/jats:italic>, the final member of the Arabidopsis SEC1 clade, did not enhance the <jats:italic>sec1a<\/jats:italic> phenotype. Thus, SEC1A is the major SM protein promoting pollen germination and tube elongation, but in its absence KEULE can partially supply this activity. When we examined the expression of the SM protein family in other species for which pollen expression data were available, we found that at least one Sec1\u2010like protein was highly expressed in pollen samples, suggesting a conserved role in pollen fertility in other species.<\/jats:p>","DOI":"10.1111\/tpj.15742","type":"journal-article","created":{"date-parts":[[2022,3,20]],"date-time":"2022-03-20T08:44:46Z","timestamp":1647765886000},"page":"1353-1369","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["<scp><i>SEC1A<\/i><\/scp> is a major Arabidopsis Sec1\/Munc18 gene in vesicle trafficking during pollen tube tip growth"],"prefix":"10.1111","volume":"110","author":[{"given":"Steven","family":"Beuder","sequence":"first","affiliation":[{"name":"Department of Molecular, Cellular and Developmental Biology University of Michigan  Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia","family":"Lara\u2010Mondrag\u00f3n","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular and Developmental Biology University of Michigan  Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandria","family":"Dorchak","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular and Developmental Biology University of Michigan  Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1470-0596","authenticated-orcid":false,"given":"Cora A.","family":"MacAlister","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular and Developmental Biology University of Michigan  Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,4,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1086391"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.152.3.531"},{"issue":"3","key":"e_1_2_9_4_1","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1007\/PL00008594","article-title":"The KEULE gene is involved in cytokinesis in Arabidopsis","volume":"253","author":"Assaad F.F.","year":"1996","journal-title":"Molecular and General Genetics"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2016.65"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.117.2.407"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006095023050"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.14808"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-0672-8_7"},{"key":"e_1_2_9_10_1","first-page":"980","article-title":"Exocyst SEC3 and phosphoinositides define sites of exocytosis in pollen tube initiation and growth","volume":"172","author":"Bloch D.","year":"2016","journal-title":"Plant Physiology"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-006-0372-3"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(00)00156-8"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1106189108"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0711980105"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.146.2.333"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2020.599247"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.4137\/BCI.S31353"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.105.062273"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/aob\/mcs256"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erx032"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.3835\/plantgenome2011.05.0015"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcn197"},{"key":"e_1_2_9_23_1","first-page":"164","article-title":"PHYLIP \u2010 phylogeny inference package (version 3.2)","volume":"5","author":"Felsenstein J.","year":"1989","journal-title":"Cladistics"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.20.00837"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.089060.108"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.108.059105"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2017.00005"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.12315"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2004-5-11-r85"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00497-015-0265-3"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1722611115"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.112.108506"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2015.053"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr1047"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.13312"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2171"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00497-021-00408-1"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.19.00965"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep40279"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.112.211441"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.13079"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.19.01340"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/35006120"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1345"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1351"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2012.03.002"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.114.14.2685"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2021.645219"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.021634"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000621"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1042\/BCJ20190050"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:PLAN.0000009297.37235.4a"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.615447"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiab049"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.124.4.1558"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.13.3.659"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004970050158"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.004630"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2009.04060.x"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2229-10-179"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.13659"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90270-4"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcaa099"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1161748"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.4161\/psb.6.5.14423"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-11-338"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10265-006-0048-x"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.108.134361"}],"container-title":["The Plant Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tpj.15742","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/tpj.15742","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tpj.15742","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tpj.15742","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T12:21:46Z","timestamp":1692879706000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tpj.15742"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,6]]},"references-count":67,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2022,6]]}},"alternative-id":["10.1111\/tpj.15742"],"URL":"https:\/\/doi.org\/10.1111\/tpj.15742","archive":["Portico"],"relation":{},"ISSN":["0960-7412","1365-313X"],"issn-type":[{"value":"0960-7412","type":"print"},{"value":"1365-313X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,6]]},"assertion":[{"value":"2021-09-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-03-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-04-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}