{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T01:19:34Z","timestamp":1787620774315,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,4,16]],"date-time":"2022-04-16T00:00:00Z","timestamp":1650067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100011254","name":"National Key Laboratory of Plant Molecular Genetics","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011254","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31900217"],"award-info":[{"award-number":["31900217"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    The essential micronutrient manganese (Mn) in plants regulates multiple biological processes including photosynthesis and oxidative stress. Some Natural Resistance\u2010Associated Macrophage Proteins (NRAMPs) have been reported to play critical roles in Mn uptake and reutilization in low Mn conditions. NRAMP6 was demonstrated to regulate cadmium tolerance and iron utilization in Arabidopsis. Nevertheless, it is unclear whether NRAMP6 plays a role in Mn nutrition. Here, we report that NRAMP6 cooperates with NRAMP1 in Mn utilization. Mutation of\n                    <jats:italic>NRAMP6<\/jats:italic>\n                    in\n                    <jats:italic>nramp1<\/jats:italic>\n                    but not in a wild\u2010type background reduces root growth and Mn translocation from the roots to shoots under Mn deficient conditions. Grafting experiments revealed that\n                    <jats:italic>NRAMP6<\/jats:italic>\n                    expression in both the roots and shoots is required for root growth and Mn translocation under Mn deficiency. We also showed that NRAMP1 could replace NRAMP6 to sustain root growth under Mn deficiency, but not\n                    <jats:italic>vice versa<\/jats:italic>\n                    . Mn deficiency does not affect the transcript level of\n                    <jats:italic>NRAMP6<\/jats:italic>\n                    , but is able to increase and decrease the protein accumulation of NRAMP6 in roots and shoots, respectively. Furthermore, NRAMP6 can be localized to both the plasma membrane and endomembranes including the endoplasmic reticulum, and Mn deficiency enhances the localization of NRAMP6 to the plasma membrane in Arabidopsis plants. NRAMP6 could rescue the defective growth of the yeast mutant \u0394\n                    <jats:italic>smf2<\/jats:italic>\n                    , which is deficient in endomembrane Mn transport. Our results reveal the important role of NRAMP6 in Mn nutrition and in the long\u2010distance signaling between the roots and shoots under Mn deficient conditions.\n                  <\/jats:p>","DOI":"10.1111\/tpj.15754","type":"journal-article","created":{"date-parts":[[2022,4,2]],"date-time":"2022-04-02T01:15:03Z","timestamp":1648862103000},"page":"1564-1577","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["<scp>NRAMP6<\/scp>\n                    and\n                    <scp>NRAMP1<\/scp>\n                    cooperatively regulate root growth and manganese translocation under manganese deficiency in Arabidopsis"],"prefix":"10.1111","volume":"110","author":[{"given":"Lun","family":"Li","sequence":"first","affiliation":[{"name":"Shanghai Center for Plant Stress Biology &amp; National Key Laboratory of Plant Molecular Genetics Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences  Shanghai 200032 China"},{"name":"State Key Laboratory of Crop Genetics and Germplasm Enhancement College of Resources and Environmental Science, Nanjing Agricultural University  Nanjing 210095 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongzheng","family":"Zhu","sequence":"additional","affiliation":[{"name":"Shanghai Center for Plant Stress Biology &amp; National Key Laboratory of Plant Molecular Genetics Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences  Shanghai 200032 China"},{"name":"University of the Chinese Academy of Sciences  Beijing 100049 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonghui","family":"Liao","sequence":"additional","affiliation":[{"name":"Shanghai Center for Plant Stress Biology &amp; National Key Laboratory of Plant Molecular Genetics Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences  Shanghai 200032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changhong","family":"Yang","sequence":"additional","affiliation":[{"name":"Shanghai Center for Plant Stress Biology &amp; National Key Laboratory of Plant Molecular Genetics Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences  Shanghai 200032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ni","family":"Fan","sequence":"additional","affiliation":[{"name":"Shanghai Center for Plant Stress Biology &amp; National Key Laboratory of Plant Molecular Genetics Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences  Shanghai 200032 China"},{"name":"University of the Chinese Academy of Sciences  Beijing 100049 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanghai Center for Plant Stress Biology &amp; National Key Laboratory of Plant Molecular Genetics Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences  Shanghai 200032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoki","family":"Yamaji","sequence":"additional","affiliation":[{"name":"Institute of Plant Science and Resources Okayama University  Chuo 2\u201020\u20101 Kurashiki 710\u20100046 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00e9on","family":"Dirick","sequence":"additional","affiliation":[{"name":"IPSiM, Univ Montpellier CNRS, INRAE, Institut Agro  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3411-827X","authenticated-orcid":false,"given":"Jian Feng","family":"Ma","sequence":"additional","affiliation":[{"name":"Institute of Plant Science and Resources Okayama University  Chuo 2\u201020\u20101 Kurashiki 710\u20100046 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Curie","sequence":"additional","affiliation":[{"name":"IPSiM, Univ Montpellier CNRS, INRAE, Institut Agro  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1486-741X","authenticated-orcid":false,"given":"Chao\u2010Feng","family":"Huang","sequence":"additional","affiliation":[{"name":"Shanghai Center for Plant Stress Biology &amp; National Key Laboratory of Plant Molecular Genetics Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences  Shanghai 200032 China"},{"name":"State Key Laboratory of Crop Genetics and Germplasm Enhancement College of Resources and Environmental Science, Nanjing Agricultural University  Nanjing 210095 China"},{"name":"University of the Chinese Academy of Sciences  Beijing 100049 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