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The rice (<jats:italic>Oryza sativa<\/jats:italic>) ubiquitin ligases OsHRZ1 and OsHRZ2 cause overall repression of these iron\u2010related genes at the transcript level, but their protein\u2010level regulation is unclear. We conducted a proteome analysis to identify key regulators whose abundance was regulated by OsHRZs at the protein level. In response to iron deficiency or <jats:italic>OsHRZ<\/jats:italic> knockdown, many genes showed differential regulation between the transcript and protein levels, including the TGA\u2010type basic leucine zipper transcription factor OsbZIP83. We also identified two glutaredoxins, OsGRX6 and OsGRX9, as OsHRZ\u2010interacting proteins in yeast and plant cells. OsGRX6 also interacted with OsbZIP83. Our <jats:italic>in\u2009vitro<\/jats:italic> degradation assay suggested that OsbZIP83, OsGRX6 and OsGRX9 proteins are subjected to 26S proteasome\u2010 and OsHRZ\u2010dependent degradation. Proteome analysis and our <jats:italic>in\u2009vitro<\/jats:italic> degradation assay also suggested that OsbZIP83 protein was preferentially degraded under iron\u2010deficient conditions in rice roots. Transgenic rice lines overexpressing <jats:italic>OsGRX9<\/jats:italic> and <jats:italic>OsbZIP83<\/jats:italic> showed improved tolerance to iron deficiency. Expression of iron\u2010related genes was affected in the <jats:italic>OsGRX9<\/jats:italic> and <jats:italic>OsGRX6<\/jats:italic> knockdown lines, suggesting disturbed iron utilization and signaling. <jats:italic>OsbZIP83<\/jats:italic> overexpression lines showed enhanced expression of <jats:italic>OsYSL2<\/jats:italic> and <jats:italic>OsNAS3<\/jats:italic>, which are involved in internal iron translocation, in addition to <jats:italic>OsGRX9<\/jats:italic> and genes related to phytoalexin biosynthesis and the salicylic acid pathway. The results suggest that OsbZIP83, OsGRX6 and OsGRX9 facilitate iron utilization downstream of the OsHRZ pathway.<\/jats:p>","DOI":"10.1111\/tpj.15767","type":"journal-article","created":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T05:33:35Z","timestamp":1649741615000},"page":"1731-1750","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["The basic leucine zipper transcription factor <scp>OsbZIP83<\/scp> and the glutaredoxins <scp>OsGRX6<\/scp> and <scp>OsGRX9<\/scp> facilitate rice iron utilization under the control of <scp>OsHRZ<\/scp> ubiquitin ligases"],"prefix":"10.1111","volume":"110","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7118-6955","authenticated-orcid":false,"given":"Takanori","family":"Kobayashi","sequence":"first","affiliation":[{"name":"Research Institute for Bioresources and Biotechnology Ishikawa Prefectural University  1\u2010308 Suematsu Nonoichi Ishikawa 921\u20108836 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