{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T05:36:33Z","timestamp":1791005793433,"version":"4.1.0"},"reference-count":67,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,12,19]],"date-time":"2022-12-19T00:00:00Z","timestamp":1671408000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31572193"],"award-info":[{"award-number":["31572193"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31760584"],"award-info":[{"award-number":["31760584"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Formate dehydrogenase (FDH; EC 1.2.1.2.) has been implicated in plant responses to a variety of stresses, including aluminum (Al) stress in acidic soils. However, the role of this enzyme in Al tolerance is not yet fully understood, and how <jats:italic>FDH<\/jats:italic> gene expression is regulated is unknown. Here, we report the identification and functional characterization of the tomato (<jats:italic>Solanum lycopersicum<\/jats:italic>) <jats:italic>SlFDH<\/jats:italic> gene. <jats:italic>SlFDH<\/jats:italic> encodes a mitochondria\u2010localized FDH with <jats:italic>K<\/jats:italic><jats:sub>m<\/jats:sub> values of 2.087\u00a0m<jats:sc>m<\/jats:sc> formate and 29.1\u00a0\u03bc<jats:sc>m<\/jats:sc> NAD<jats:sup>+<\/jats:sup>. Al induced the expression of <jats:italic>SlFDH<\/jats:italic> in tomato root tips, but other metals did not, as determined by quantitative reverse transcriptase\u2010polymerase chain reaction. CRISPR\/Cas9\u2010generated <jats:italic>SlFDH<\/jats:italic> knockout lines were more sensitive to Al stress and formate than wild\u2010type plants. Formate failed to induce <jats:italic>SlFDH<\/jats:italic> expression in the tomato root apex, but NAD<jats:sup>+<\/jats:sup> accumulated in response to Al stress. Co\u2010expression network analysis and interaction analysis between genomic DNA and transcription factors (TFs) using PlantRegMap identified seven TFs that might regulate <jats:italic>SlFDH<\/jats:italic> expression. One of these TFs, SlSTOP1, positively regulated <jats:italic>SlFDH<\/jats:italic> expression by directly binding to its promoter, as demonstrated by a dual\u2010luciferase reporter assay and electrophoretic mobility shift assay. The Al\u2010induced expression of <jats:italic>SlFDH<\/jats:italic> was completely abolished in <jats:italic>Slstop1<\/jats:italic> mutants, indicating that SlSTOP1 is a core regulator of <jats:italic>SlFDH<\/jats:italic> expression under Al stress. Taken together, our findings demonstrate that SlFDH plays a role in Al tolerance and reveal the transcriptional regulatory mechanism of <jats:italic>SlFDH<\/jats:italic> expression in response to Al stress in tomato.<\/jats:p>","DOI":"10.1111\/tpj.16054","type":"journal-article","created":{"date-parts":[[2022,12,6]],"date-time":"2022-12-06T06:29:10Z","timestamp":1670308150000},"page":"387-401","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Involvement of <scp>SlSTOP1<\/scp> regulated <i>SlFDH<\/i> expression in aluminum tolerance by reducing <scp>NAD<\/scp><sup>+<\/sup> to <scp>NADH<\/scp> in the tomato root apex"],"prefix":"10.1111","volume":"113","author":[{"given":"Qiyu","family":"He","sequence":"first","affiliation":[{"name":"State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences Zhejiang University  Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianfeng","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Horticulture Zhejiang University  Zijingang Campus, 866 Yuhangtang Road Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengfei","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences Zhejiang University  Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huihui","family":"Zhu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences Zhejiang University  Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhanqi","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, College of Life Sciences Huzhou University  Huzhou 313000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Fan","sequence":"additional","affiliation":[{"name":"College of Horticulture and Landscape Yunnan Agricultural University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0385-5787","authenticated-orcid":false,"given":"Jian Li","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences Zhejiang University  Hangzhou 310058 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