{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T09:38:49Z","timestamp":1787564329960,"version":"build-2736575974"},"reference-count":60,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,1,9]],"date-time":"2023-01-09T00:00:00Z","timestamp":1673222400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2019YFD1000502"],"award-info":[{"award-number":["2019YFD1000502"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2018YFD1000502"],"award-info":[{"award-number":["2018YFD1000502"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31770642"],"award-info":[{"award-number":["31770642"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    Tapping panel dryness (TPD) is a century\u2010old problem that has plagued the natural rubber production of\n                    <jats:italic>Hevea brasiliensis<\/jats:italic>\n                    . TPD may result from self\u2010protective mechanisms of\n                    <jats:italic>H. brasiliensis<\/jats:italic>\n                    in response to stresses such as excessive hormone stimulation and mechanical wounding (bark tapping). It has been hypothesized that TPD impairs rubber biosynthesis; however, the underlying mechanisms remain poorly understood. In the present study, we firstly verified that TPD\u2010affected rubber trees exhibited lower rubber biosynthesis activity and greater rubber molecular weight compared to healthy rubber trees. We then demonstrated that\n                    <jats:italic>HbFPS1<\/jats:italic>\n                    , a key gene of rubber biosynthesis, and its expression products were downregulated in the latex of TPD\u2010affected rubber trees, as revealed by transcriptome sequencing and iTRAQ\u2010based proteome analysis. We further discovered that the farnesyl diphosphate synthase HbFPS1 could be recruited to small rubber particles by HbSRPP1 through protein\u2013protein interactions to catalyze farnesyl diphosphate (FPP) synthesis and facilitate rubber biosynthesis initiation. FPP content in the latex of TPD\u2010affected rubber trees was significantly decreased with the downregulation of HbFPS1, ultimately resulting in abnormal development of rubber particles, decreased rubber biosynthesis activity, and increased rubber molecular weight. Upstream regulator assays indicated that a novel regulator, MYB2\u2010like, may be an important regulator of downregulation of\n                    <jats:italic>HbFPS1<\/jats:italic>\n                    in the latex of TPD\u2010affected rubber trees. Our findings not only provide new directions for studying the molecular events involved in rubber biosynthesis and TPD syndrome and contribute to rubber management strategies, but also broaden our knowledge of plant isoprenoid metabolism and its regulatory networks.\n                  <\/jats:p>","DOI":"10.1111\/tpj.16063","type":"journal-article","created":{"date-parts":[[2022,12,16]],"date-time":"2022-12-16T07:51:38Z","timestamp":1671177098000},"page":"504-520","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Downregulation of\n                    <scp>\n                      <i>HbFPS1<\/i>\n                    <\/scp>\n                    affects rubber biosynthesis of\n                    <i>Hevea brasiliensis<\/i>\n                    suffering from tapping panel dryness"],"prefix":"10.1111","volume":"113","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8796-9081","authenticated-orcid":false,"given":"Zhiyi","family":"Nie","sequence":"first","affiliation":[{"name":"Rubber Research Institute &amp; Key Laboratory of Biology and Genetic Resources of Rubber trees Ministry of Agriculture and Rural Affairs of the People's Republic of China, Chinese Academy of Tropical Agricultural Sciences Haikou 571101 Hainan China"},{"name":"Key Laboratory of Materials Engineering for High Performance Natural Rubber, Hainnan Province Chinese Academy of Tropical Agricultural Sciences Haikou 571101 Hainan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guijuan","family":"Kang","sequence":"additional","affiliation":[{"name":"Rubber Research Institute &amp; Key Laboratory of Biology and Genetic Resources of Rubber trees Ministry of Agriculture and Rural Affairs of the People's Republic of China, Chinese Academy of Tropical Agricultural Sciences Haikou 571101 Hainan China"},{"name":"Key Laboratory of Materials Engineering for High Performance Natural Rubber, Hainnan Province Chinese Academy of Tropical Agricultural Sciences Haikou 571101 Hainan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Yan","sequence":"additional","affiliation":[{"name":"Rubber Research Institute &amp; Key Laboratory of Biology and Genetic Resources of Rubber trees Ministry of Agriculture and Rural Affairs of the People's Republic of China, Chinese Academy of Tropical Agricultural Sciences Haikou 571101 Hainan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huaide","family":"Qin","sequence":"additional","affiliation":[{"name":"Rubber Research Institute &amp; Key Laboratory of Biology and Genetic Resources of Rubber trees Ministry of Agriculture and Rural Affairs of the People's Republic of China, Chinese Academy of Tropical Agricultural Sciences Haikou 571101 Hainan China"},{"name":"Key Laboratory of Materials Engineering for High Performance Natural Rubber, Hainnan Province Chinese Academy of Tropical Agricultural Sciences Haikou 571101 Hainan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lifu","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Scientific and Technical Information Chinese Academy of Tropical Agricultural Sciences Haikou 571101 Hainan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rizhong","family":"Zeng","sequence":"additional","affiliation":[{"name":"Rubber Research Institute &amp; Key Laboratory of Biology and Genetic Resources of Rubber trees Ministry of Agriculture and Rural Affairs of the People's Republic of China, Chinese Academy of Tropical Agricultural Sciences Haikou 571101 Hainan China"},{"name":"Key Laboratory of Materials Engineering for High Performance Natural Rubber, Hainnan Province Chinese Academy of Tropical Agricultural Sciences Haikou 571101 Hainan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,1,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00020805"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erx331"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1020719420867"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2016.08.006"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/err363"},{"key":"e_1_2_9_7_1","first-page":"431","volume-title":"Physiology of rubber tree latex","author":"Chrestin H.","year":"1989"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1993.tb18374.x"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(01)00097-8"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm000034z"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-013-0047-y"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2015.11.012"},{"key":"e_1_2_9_13_1","first-page":"59","volume-title":"Physiology of rubber tree latex","author":"d'Auzac J.","year":"1989"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1071\/BT11070"},{"key":"e_1_2_9_15_1","first-page":"15","volume-title":"Physiology of rubber tree latex","author":"Fa\u00ff E.","year":"1989"},{"key":"e_1_2_9_16_1","first-page":"4","volume-title":"Physiology of rubber tree latex","author":"Fa\u00ff E.","year":"1989"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/ery169"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2016.00789"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nplants.2015.48"},{"key":"e_1_2_9_20_1","first-page":"43","article-title":"Cause of disease and hypothesis on tapping panel dryness of Hevea brasiliensis. 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