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CNGC\u2010mediated Ca\n                    <jats:sup>2+<\/jats:sup>\n                    elevations are essential for plant immune defense and high temperature thermosensing. In the present study, we evaluated phenotypes of\n                    <jats:italic>CNGC2<\/jats:italic>\n                    ,\n                    <jats:italic>CNGC4<\/jats:italic>\n                    ,\n                    <jats:italic>CNGC6<\/jats:italic>\n                    , and\n                    <jats:italic>CNGC12<\/jats:italic>\n                    null mutants in these two pathways. It is shown CNGC2, CNGC4, and CNGC6 physically interact\n                    <jats:italic>in\u00a0vivo<\/jats:italic>\n                    , whereas CNGC12 does not. CNGC involvement in immune signaling was evaluated by monitoring mutant response to elicitor peptide Pep3. Pep3 response cascades involving CNGCs included mitogen\u2010activated kinase activation mediated by Ca\n                    <jats:sup>2+<\/jats:sup>\n                    \u2010dependent protein kinase phosphorylation. Pep3\u2010induced reactive oxygen species generation was impaired in\n                    <jats:italic>cngc2<\/jats:italic>\n                    ,\n                    <jats:italic>cngc4<\/jats:italic>\n                    , and\n                    <jats:italic>cngc6<\/jats:italic>\n                    , but not in\n                    <jats:italic>cngc12<\/jats:italic>\n                    , suggesting that CNGC2, CNGC4, and CNGC6 (which physically interact) may be components of a multimeric CNGC channel complex for immune signaling. However, unlike\n                    <jats:italic>cngc2<\/jats:italic>\n                    and\n                    <jats:italic>cngc4<\/jats:italic>\n                    ,\n                    <jats:italic>cngc6<\/jats:italic>\n                    is not sensitive to high Ca\n                    <jats:sup>2+<\/jats:sup>\n                    and displays no pleiotropic dwarfism. All four\n                    <jats:italic>cngc<\/jats:italic>\n                    mutants showed thermotolerance compared to wild\u2010type, although CNGC12 does not interact with the other three CNGCs. These results imply that physically interacting CNGCs may, in some cases, function in a signaling cascade as components of a heteromeric channel complex, although this may not be the case in other signaling pathways.\n                  <\/jats:p>","DOI":"10.1111\/tpj.16106","type":"journal-article","created":{"date-parts":[[2023,1,12]],"date-time":"2023-01-12T05:22:09Z","timestamp":1673500929000},"page":"1223-1236","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Phenotypes of cyclic nucleotide\u2010gated cation channel mutants: probing the nature of native channels"],"prefix":"10.1111","volume":"113","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6673-0549","authenticated-orcid":false,"given":"Yi","family":"Ma","sequence":"first","affiliation":[{"name":"Department of Plant Science and Landscape Architecture, Agricultural Biotechnology Laboratory University of Connecticut  Storrs CT 06269 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keila","family":"Garrido","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rashid","family":"Ali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerald A.","family":"Berkowitz","sequence":"additional","affiliation":[{"name":"Department of Plant Science and Landscape Architecture, Agricultural Biotechnology Laboratory University of Connecticut  Storrs CT 06269 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