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The synthesis of their complex carbohydrates is initiated by a family of hydroxyproline galactosyltransferase (Hyp\u2010GALT) enzymes which add the first galactose to Hyp residues in the protein backbone. Eight Hyp\u2010GALT enzymes have been identified so far, and in the present work a mutant affecting five of these enzymes (<jats:italic>galt2galt5galt7galt8galt9<\/jats:italic>) was analyzed regarding the reproductive process. The <jats:italic>galt25789<\/jats:italic> mutant presented a low seed set, and reciprocal crosses indicated a significant female gametophytic contribution to this mutant phenotype. Mutant ovules revealed abnormal callose accumulation inside the embryo sac and integument defects at the micropylar region culminating in defects in pollen tube reception. In addition, immunolocalization and biochemical analyses allowed the detection of a reduction in the amount of glucuronic acid in mutant ovary AGPs. Dramatically low amounts of high\u2010molecular\u2010weight Hyp\u2010<jats:italic>O<\/jats:italic>\u2010glycosides obtained following size exclusion chromatography of base\u2010hydrolyzed mutant AGPs compared to the wild type indicated the presence of underglycosylated AGPs in the <jats:italic>galt25789<\/jats:italic> mutant, while the monosaccharide composition of these Hyp\u2010<jats:italic>O<\/jats:italic>\u2010glycosides displayed no significant changes compared to the wild\u2010type Hyp\u2010<jats:italic>O<\/jats:italic>\u2010glycosides. The present work demonstrates the functional importance of the carbohydrate moieties of AGPs in ovule development and pollen\u2013pistil interactions.<\/jats:p>","DOI":"10.1111\/tpj.16141","type":"journal-article","created":{"date-parts":[[2023,2,13]],"date-time":"2023-02-13T07:04:59Z","timestamp":1676271899000},"page":"371-389","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Type II arabinogalactans initiated by hydroxyproline\u2010O\u2010galactosyltransferases play important roles in pollen\u2013pistil interactions"],"prefix":"10.1111","volume":"114","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5405-4587","authenticated-orcid":false,"given":"Diana","family":"Moreira","sequence":"first","affiliation":[{"name":"LAQV\/REQUIMTE, Departamento de Biologia, Faculdade de Ci\u00eancias Universidade do Porto  Rua do Campo Alegre s\/n 4169\u2010007 Porto 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