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Fe\u2010limitation significantly reduces crop yield and adversely impacts on human nutrition. Owing to limited bioavailability of Fe in soil, plants have adapted different strategies that not only regulate Fe\u2010uptake and homeostasis but also bring modifications in root system architecture to enhance survival. Understanding the molecular mechanism underlying the root growth responses will have critical implications for plant breeding. Fe\u2010uptake is regulated by a cascade of basic helix\u2013loop\u2013helix (bHLH) transcription factors (TFs) in plants. In this study, we report that HY5 (Elongated Hypocotyl 5), a member of the basic leucine zipper (bZIP) family of TFs, plays an important role in the Fe\u2010deficiency signaling pathway in <jats:italic>Arabidopsis thaliana<\/jats:italic>. The <jats:italic>hy5<\/jats:italic> mutant failed to mount optimum Fe\u2010deficiency responses, and displayed root growth defects under Fe\u2010limitation. Our analysis revealed that the induction of the genes involved in Fe\u2010uptake pathway (<jats:italic>FIT\u2010FER\u2010LIKE IRON DEFICIENCY\u2010INDUCED TRANSCRIPTION FACTOR<\/jats:italic>, <jats:italic>FRO2\u2010FERRIC REDUCTION OXIDASE 2<\/jats:italic> and <jats:italic>IRT1\u2010IRON\u2010REGULATED TRANSPORTER1<\/jats:italic>) is reduced in the <jats:italic>hy5<\/jats:italic> mutant as compared with the wild\u2010type plants under Fe\u2010deficiency. Moreover, we also found that the expression of coumarin biosynthesis genes is affected in the <jats:italic>hy5<\/jats:italic> mutant under Fe\u2010deficiency. Our results also showed that <jats:italic>HY5<\/jats:italic> negatively regulates BRUTUS (<jats:italic>BTS<\/jats:italic>) and POPEYE (<jats:italic>PYE<\/jats:italic>). Chromatin immunoprecipitation followed by quantitative polymerase chain reaction revealed direct binding of HY5 to the promoters of <jats:italic>BTS<\/jats:italic>, <jats:italic>FRO2<\/jats:italic> and <jats:italic>PYE<\/jats:italic>. Altogether, our results showed that <jats:italic>HY5<\/jats:italic> plays an important role in the regulation of Fe\u2010deficiency responses in <jats:italic>Arabidopsis<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/tpj.16191","type":"journal-article","created":{"date-parts":[[2023,3,15]],"date-time":"2023-03-15T14:10:04Z","timestamp":1678889404000},"page":"1267-1284","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["<scp>ELONGATED HYPOCOTYL<\/scp> 5 regulates <scp><i>BRUTUS<\/i><\/scp> and affects iron acquisition and homeostasis in <i>Arabidopsis thaliana<\/i>"],"prefix":"10.1111","volume":"114","author":[{"given":"Samriti","family":"Mankotia","sequence":"first","affiliation":[{"name":"Department of Biological Sciences Indian Institute of Science Education and Research (IISER) Mohali  SAS Nagar Mohali Punjab 140306 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