{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T17:07:37Z","timestamp":1791824857704,"version":"4.3.4"},"reference-count":80,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,4,10]],"date-time":"2023-04-10T00:00:00Z","timestamp":1681084800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003819","name":"Natural Science Foundation of Hubei Province","doi-asserted-by":"publisher","award":["2022CFB168"],"award-info":[{"award-number":["2022CFB168"]}],"id":[{"id":"10.13039\/501100003819","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Hydrogen sulfide (H<jats:sub>2<\/jats:sub>S) promotes plant tolerance against various environmental cues, and <jats:sc>d<\/jats:sc>\u2010cysteine desulfhydrase (DCD) is an enzymatic source of H<jats:sub>2<\/jats:sub>S to enhance abiotic stress resistance. However, the role of DCD\u2010mediated H<jats:sub>2<\/jats:sub>S production in root growth under abiotic stress remains to be further elucidated. Here, we report that DCD\u2010mediated H<jats:sub>2<\/jats:sub>S production alleviates osmotic stress\u2010mediated root growth inhibition by promoting auxin homeostasis. Osmotic stress up\u2010regulated <jats:italic>DCD<\/jats:italic> gene transcript and DCD protein levels and thus H<jats:sub>2<\/jats:sub>S production in roots. When subjected to osmotic stress, a <jats:italic>dcd<\/jats:italic> mutant showed more severe root growth inhibition, whereas the transgenic lines <jats:italic>DCDox<\/jats:italic> overexpressing <jats:italic>DCD<\/jats:italic> exhibited less sensitivity to osmotic stress in terms of longer root compared to the wild\u2010type. Moreover, osmotic stress inhibited root growth through repressing auxin signaling, whereas H<jats:sub>2<\/jats:sub>S treatment significantly alleviated osmotic stress\u2010mediated inhibition of auxin. Under osmotic stress, auxin accumulation was increased in <jats:italic>DCDox<\/jats:italic> but decreased in <jats:italic>dcd<\/jats:italic> mutant. H<jats:sub>2<\/jats:sub>S promoted auxin biosynthesis gene expression and auxin efflux carrier PIN\u2010FORMED 1 (PIN1) protein level under osmotic stress. Taken together, our results reveal that mannitol\u2010induced <jats:italic>DCD<\/jats:italic> and H<jats:sub>2<\/jats:sub>S in roots promote auxin homeostasis, contributing to alleviating the inhibition of root growth under osmotic stress.<\/jats:p>","DOI":"10.1111\/tpj.16198","type":"journal-article","created":{"date-parts":[[2023,3,23]],"date-time":"2023-03-23T02:49:51Z","timestamp":1679539791000},"page":"1369-1384","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Hydrogen sulfide alleviates osmotic stress\u2010induced root growth inhibition by promoting auxin homeostasis"],"prefix":"10.1111","volume":"114","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5243-5899","authenticated-orcid":false,"given":"Zhi\u2010Xin","family":"Xiang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Hybrid Rice, College of Life Sciences Wuhan University  Wuhan 430072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Hybrid Rice, College of Life Sciences Wuhan University  Wuhan 430072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3540-6935","authenticated-orcid":false,"given":"Ying\u2010Tang","family":"Lu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Hybrid Rice, College of Life Sciences Wuhan University  Wuhan 430072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8577-3584","authenticated-orcid":false,"given":"Ting\u2010Ting","family":"Yuan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Hybrid Rice, College of Life Sciences Wuhan University  Wuhan 430072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,4,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2020.110978"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00924-3"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2005.07.014"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03184"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes10120983"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep12516"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11104-012-1275-7"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2020.01.004"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0110904"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envexpbot.2021.104693"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1415106"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313x.1999.00620.x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.12779"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envexpbot.2022.104856"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11738-019-2918-6"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1000672107"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2013.00397"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.13627"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2013.12.024"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41477-018-0190-1"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.7b00205"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1015248926412"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02085"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06206"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.12144"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2010.03465.x"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06215"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.12982"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.12004"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/jexbot\/51.350.1595"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2018.01172"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1164147"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2018.01517"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep08251"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2012.10.017"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21041513"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/aob\/mcl006"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2018.05.011"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.15351"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a040014"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.18.00838"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erv287"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.15.00030"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.12.14.2175"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/plb.13417"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2021.07.016"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2021.117958"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05130"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2012.06.050"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-016-0476-5"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a001537"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2020.122061"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-965424"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2005.04567.x"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.13882"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.19.00826"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2014.06.008"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.12302"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/ppl.13028"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.108.064303"},{"key":"e_1_2_9_62_1","first-page":"1963","article-title":"Aux\/IAA proteins repress expression of reporter genes containing natural and highly active synthetic auxin response elements","volume":"9","author":"Ulmasov T.","year":"1997","journal-title":"The Plant Cell"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02027"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2019.12.001"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21134593"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.12597"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.17687"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.12233"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.18.00455"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-7909.2009.00885.x"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.13685"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.13022"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-01046-2"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.19.01504"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-020-03362-w"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.291.5502.306"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10265-020-01182-3"},{"key":"e_1_2_9_78_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2021.03.007"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcu217"},{"key":"e_1_2_9_80_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5266(00)00192-8"},{"key":"e_1_2_9_81_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erp290"}],"container-title":["The Plant Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tpj.16198","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tpj.16198","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,28]],"date-time":"2024-02-28T17:53:30Z","timestamp":1709142810000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tpj.16198"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,10]]},"references-count":80,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,6]]}},"alternative-id":["10.1111\/tpj.16198"],"URL":"https:\/\/doi.org\/10.1111\/tpj.16198","archive":["Portico"],"relation":{},"ISSN":["0960-7412","1365-313X"],"issn-type":[{"value":"0960-7412","type":"print"},{"value":"1365-313X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,10]]},"assertion":[{"value":"2022-07-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}