{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T03:11:45Z","timestamp":1786763505735,"version":"build-2736575974"},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T00:00:00Z","timestamp":1692748800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFD1400800"],"award-info":[{"award-number":["2021YFD1400800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2018A030313244"],"award-info":[{"award-number":["2018A030313244"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Citrus production is severely threatened by the devastating Huanglongbing (HLB) disease globally. By studying and analyzing the defensive behaviors of an HLB\u2010tolerant citrus cultivar \u2018Shatangju\u2019, we discovered that citrus can sense <jats:italic>Candidatus<\/jats:italic> Liberibacter asiaticus (<jats:italic>C<\/jats:italic>Las) infection and induce immune responses against HLB, which can be further strengthened by both endogenously produced and exogenously applied methyl salicylate (MeSA). This immune circuit is turned on by an miR2977\u2010<jats:italic>SAMT<\/jats:italic> (encoding a citrus Salicylate [SA] <jats:italic>O<\/jats:italic>\u2010methyltransferase) cascade, by which <jats:italic>C<\/jats:italic>Las infection leads to more in planta MeSA production and aerial emission. We provided both transgenic and multi\u2010year trail evidences that MeSA is an effective community immune signal. Ambient MeSA accumulation and foliage application can effectively induce defense gene expression and significantly boost citrus performance. We also found that miRNAs are battle fields between citrus and <jats:italic>C<\/jats:italic>Las, and about 30% of the differential gene expression upon <jats:italic>C<\/jats:italic>Las infection are regulated by miRNAs. Furthermore, <jats:italic>C<\/jats:italic>Las hijacks host key processes by manipulating key citrus miRNAs, and citrus employs miRNAs that coordinately regulate defense\u2010related genes. Based on our results, we proposed that miRNAs and associated components are key targets for engineering or breeding resistant citrus varieties. We anticipate that MeSA\u2010based management, either induced expression or external application, would be a promising tool for HLB control.<\/jats:p>","DOI":"10.1111\/tpj.16426","type":"journal-article","created":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T06:01:24Z","timestamp":1692856884000},"page":"1309-1324","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Small <scp>RNAs<\/scp> contribute to citrus Huanglongbing tolerance by manipulating methyl salicylate signaling and exogenous methyl salicylate primes citrus groves from emerging infection"],"prefix":"10.1111","volume":"116","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4024-9970","authenticated-orcid":false,"given":"Baoping","family":"Cheng","sequence":"first","affiliation":[{"name":"Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences\/Guangdong Provincial Key Laboratory of High Technology for Plant Protection  Guangzhou 510640 China"},{"name":"Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs  Guangzhou Guangdong 510642 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Plant Protection Nanjing Agricultural University  Nanjing 210095 China"},{"name":"The Key Laboratory of Plant Immunity Nanjing Agricultural University  Nanjing 210095 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Saqib","family":"Bilal","sequence":"additional","affiliation":[{"name":"College of Plant Protection Nanjing Agricultural University  Nanjing 210095 China"},{"name":"The Key Laboratory of Plant Immunity Nanjing Agricultural University  Nanjing 210095 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Plant Protection Nanjing Agricultural University  Nanjing 210095 China"},{"name":"The Key Laboratory of Plant Immunity Nanjing Agricultural University  Nanjing 210095 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9926-0923","authenticated-orcid":false,"given":"Dongdong","family":"Niu","sequence":"additional","affiliation":[{"name":"College of Plant Protection Nanjing Agricultural University  Nanjing 210095 China"},{"name":"The Key Laboratory of Plant Immunity Nanjing Agricultural University  Nanjing 210095 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyu","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Plant Protection Nanjing Agricultural University  Nanjing 210095 China"},{"name":"The Key Laboratory of Plant Immunity Nanjing Agricultural University  Nanjing 210095 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoxia","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Plant Protection Nanjing Agricultural University  Nanjing 210095 China"},{"name":"The Key Laboratory of Plant Immunity Nanjing Agricultural University  Nanjing 210095 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aitian","family":"Peng","sequence":"additional","affiliation":[{"name":"Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences\/Guangdong Provincial Key Laboratory of High Technology for Plant Protection  Guangzhou 510640 China"},{"name":"Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs  Guangzhou Guangdong 510642 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guo","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences University of Tennessee  Knoxville Tennessee 37996 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences University of Tennessee  Knoxville Tennessee 37996 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Zeng","sequence":"additional","affiliation":[{"name":"Biozeron Biotech. Co., Ltd.  Shanghai 200120 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Lin","sequence":"additional","affiliation":[{"name":"San Joaquin Valley Agricultural Sciences Center, USDA\u2010ARS  Parlier California 93658 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayesha","family":"Baig","sequence":"additional","affiliation":[{"name":"Department of Biotechnology COMSATS University Islamabad Abbottabad Campus  Abbottabad KPK 22010 Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9293-3427","authenticated-orcid":false,"given":"Xuefeng","family":"Wang","sequence":"additional","affiliation":[{"name":"National Citrus Engineering Research Center, Citrus Research Institute Southwest University  Beibei Chongqing 400712 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7522-0444","authenticated-orcid":false,"given":"Xiuping","family":"Zou","sequence":"additional","affiliation":[{"name":"National Citrus Engineering Research Center, Citrus Research Institute Southwest University  Beibei Chongqing 400712 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9107-765X","authenticated-orcid":false,"given":"Hongwei","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Plant Protection Nanjing Agricultural University  Nanjing 210095 China"},{"name":"The Key Laboratory of Plant Immunity Nanjing Agricultural University  Nanjing 210095 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