{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T22:43:57Z","timestamp":1768517037487,"version":"3.49.0"},"reference-count":69,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T00:00:00Z","timestamp":1694822400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000308","name":"British Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000308","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/J000PR9799"],"award-info":[{"award-number":["BB\/J000PR9799"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/J00PR9796"],"award-info":[{"award-number":["BB\/J00PR9796"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004034","name":"John Innes Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004034","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002367","name":"Chinese Academy of Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002367","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001332","name":"Council for Scientific and Industrial Research, South Africa","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001332","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>The plant citrate transporters, functional in mineral nutrient uptake and homeostasis, usually belong to the multidrug and toxic compound extrusion transporter family. We identified and functionally characterized a rice (<jats:italic>Oryza sativa<\/jats:italic>) citrate transporter, OsCT1, which differs from known plant citrate transporters and is structurally close to rice silicon transporters. Domain analysis depicted that OsCT1 carries a bacterial citrate\u2010metal transporter domain, CitMHS. OsCT1 showed citrate efflux activity when expressed in <jats:italic>Xenopus laevis<\/jats:italic> oocytes and is localized to the cell plasma membrane. It is highly expressed in the shoot and reproductive tissues of rice, and its promoter activity was visible in cells surrounding the vasculature. The <jats:italic>OsCT1<\/jats:italic> knockout (KO) lines showed a reduced citrate content in the shoots and the root exudates, whereas overexpression (OE) line showed higher citrate exudation from their roots. Further, the KO and OE lines showed variations in the manganese (Mn) distribution leading to changes in their agronomical traits<jats:italic>.<\/jats:italic> Under deficient conditions (Mn\u2010sufficient conditions followed by 8\u2009days of 0\u2009\u03bc<jats:sc>m<\/jats:sc> MnCl<jats:sub>2<\/jats:sub>\u2009\u00b7\u20094H<jats:sub>2<\/jats:sub>O treatment), the supply of manganese towards the newer leaf was found to be obstructed in the KO line. There were no significant differences in phosphorus (P) distribution; however, P uptake was reduced in the KO and increased in OE lines at the vegetative stage. Further, experiments in <jats:italic>Xenopus<\/jats:italic> oocytes revealed that OsCT1 could efflux citrate with Mn. In this way, we provide insights into a mechanism of citrate\u2010metal transport in plants and its role in mineral homeostasis, which remains conserved with their bacterial counterparts.<\/jats:p>","DOI":"10.1111\/tpj.16463","type":"journal-article","created":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T13:44:28Z","timestamp":1694871868000},"page":"1748-1765","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["A citrate efflux transporter important for manganese distribution and phosphorus uptake in rice"],"prefix":"10.1111","volume":"116","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9593-7776","authenticated-orcid":false,"given":"Poonam","family":"Panchal","sequence":"first","affiliation":[{"name":"National Institute of Plant Genome Research  Aruna Asaf Ali Marg New Delhi 110067 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chitra","family":"Bhatia","sequence":"additional","affiliation":[{"name":"National Institute of Plant Genome Research  Aruna Asaf Ali Marg New Delhi 110067 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Chen","sequence":"additional","affiliation":[{"name":"Biochemistry and Metabolism Department John Innes Centre  Norwich Research Park Norwich NR4 7UH UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meenakshi","family":"Sharma","sequence":"additional","affiliation":[{"name":"National Institute of Plant Genome Research  Aruna Asaf Ali Marg New Delhi 110067 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jyoti","family":"Bhadouria","sequence":"additional","affiliation":[{"name":"National Institute of Plant Genome Research  Aruna Asaf Ali Marg New Delhi 110067 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lokesh","family":"Verma","sequence":"additional","affiliation":[{"name":"National Institute of Plant Genome Research  Aruna Asaf Ali Marg New Delhi 110067 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kanika","family":"Maurya","sequence":"additional","affiliation":[{"name":"National Institute of Plant Genome Research  Aruna Asaf Ali Marg New Delhi 110067 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony J.","family":"Miller","sequence":"additional","affiliation":[{"name":"Biochemistry and Metabolism Department John Innes Centre  Norwich Research Park Norwich NR4 7UH UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6969-5187","authenticated-orcid":false,"given":"Jitender","family":"Giri","sequence":"additional","affiliation":[{"name":"National Institute of Plant Genome Research  Aruna Asaf Ali Marg New Delhi 110067 India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,9,16]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(66)08014-5"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2006.05509.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jpln.201000085"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1534\/g3.115.025791"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erx165"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0406258101"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-7652.2009.00403.x"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.107.097162"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.13964"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:PLSO.0000030182.11473.3b"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13007-018-0329-0"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2011.03740.x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.104.045633"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/00103620600623558"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mex.2019.12.001"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0602868103"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02280176"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.17959"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ica.2012.06.025"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/00380768.2004.10408586"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2010.04158.x"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1987.tb02730.x"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1004356007312"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.708578"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12284-017-0196-8"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.107.102335"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.13.4451-4456.2005"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.22.6374-6381.2000"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.17.01538"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2007.03.012"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.3109\/10409238.2010.504701"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00456-08"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00232-001-0106-1"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.112.208934"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.106.085233"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2008.03696.x"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.17.01142"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2019.110359"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/74531"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05964"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0802361105"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-7652.2011.00658.x"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/treephys\/tpt011"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep13090"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2015.01184"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.109.067884"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erab019"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2011.00037"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/ery357"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.001495"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.111.088088"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2003.01991.x"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants8100381"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10142-012-0274-3"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-020-01047-2"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2006.02836.x"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erac192"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2007.02206.x"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1508987112"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3040.2011.02410.x"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcw026"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.16.01214"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1071\/FP09265"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2011.04757.x"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erw314"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.108.128132"},{"key":"e_1_2_9_68_1","first-page":"1","article-title":"Isolation and characterization of two phosphate\u2010solubilizing fungi from rhizosphere soil of moso bamboo and their functional capacities when exposed to different phosphorus sources and pH envFements","volume":"13","author":"Zhang Y.","year":"2018","journal-title":"PLoS One"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.109.141051"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1093\/aob\/mct135"}],"container-title":["The Plant Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tpj.16463","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,26]],"date-time":"2023-12-26T10:59:24Z","timestamp":1703588364000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tpj.16463"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,16]]},"references-count":69,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,12]]}},"alternative-id":["10.1111\/tpj.16463"],"URL":"https:\/\/doi.org\/10.1111\/tpj.16463","archive":["Portico"],"relation":{},"ISSN":["0960-7412","1365-313X"],"issn-type":[{"value":"0960-7412","type":"print"},{"value":"1365-313X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,16]]},"assertion":[{"value":"2022-11-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}