{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T06:48:37Z","timestamp":1787986117390,"version":"build-2784847793"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T00:00:00Z","timestamp":1698278400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/P012523\/1"],"award-info":[{"award-number":["BB\/P012523\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/T004363\/1"],"award-info":[{"award-number":["BB\/T004363\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/V015095\/1"],"award-info":[{"award-number":["BB\/V015095\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>The <jats:italic>Pisum sativum<\/jats:italic> (pea) mutants <jats:italic>degenerate leaves<\/jats:italic> (<jats:italic>dgl<\/jats:italic>) and <jats:italic>bronze<\/jats:italic> (<jats:italic>brz<\/jats:italic>) accumulate large amounts of iron in leaves. First described several decades ago, the two mutants have provided important insights into iron homeostasis in plants but the underlying mutations have remained unknown. Using exome sequencing we identified an in\u2010frame deletion associated with <jats:italic>dgl<\/jats:italic> in a <jats:italic>BRUTUS<\/jats:italic> homolog. The deletion is absent from wild type and the original parent line. BRUTUS belongs to a small family of E3 ubiquitin ligases acting as negative regulators of iron uptake in plants. The <jats:italic>brz<\/jats:italic> mutation was previously mapped to chromosome 4, and superimposing this region to the pea genome sequence uncovered a mutation in <jats:italic>OPT3<\/jats:italic>, encoding an oligopeptide transporter with a plant\u2010specific role in metal transport. The causal nature of the mutations was confirmed by additional genetic analyses. Identification of the mutated genes rationalizes many of the previously described phenotypes and provides new insights into shoot\u2010to\u2010root signaling of iron deficiency. Furthermore, the non\u2010lethal mutations in these essential genes suggest new strategies for biofortification of crops with iron.<\/jats:p>","DOI":"10.1111\/tpj.16514","type":"journal-article","created":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T11:27:31Z","timestamp":1698319651000},"page":"590-598","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Genetic basis of the historical iron\u2010accumulating <i>dgl<\/i> and <i>brz<\/i> mutants in pea"],"prefix":"10.1111","volume":"117","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0754-0678","authenticated-orcid":false,"given":"Sophie A.","family":"Harrington","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Metabolism John Innes Centre  Norwich NR4 7UH UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1389-6825","authenticated-orcid":false,"given":"Marina","family":"Franceschetti","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Metabolism John Innes Centre  Norwich NR4 7UH UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4738-1990","authenticated-orcid":false,"given":"Janneke","family":"Balk","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Metabolism John Innes Centre  Norwich NR4 7UH UK"},{"name":"School of Biological Sciences University of East Anglia  Norwich NR4 7TJ UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,10,26]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004250050475"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3519"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/plcell\/koad053"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcz079"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22010432"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2011.06.006"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0028-646X.2001.00302.x"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-012-1757-0"},{"key":"e_1_2_5_10_1","first-page":"9","article-title":"Improvement of the selection value gene dgl through recombination","volume":"19","author":"Gottschalk W.","year":"1987","journal-title":"Pisum Newsletter"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.104.2.649"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.110.1.329"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.93.3.976"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7MT00152E"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants9111505"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.93.2.717"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-019-0480-1"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt865"},{"key":"e_1_2_5_19_1","unstructured":"Li H.(2013)Aligning sequence reads clone sequences and assembly contigs with BWA\u2010MEM.arXiv 1303.3997."},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0960258500004293"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1679\/aohc.70.1"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/mp\/ssu067"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4324"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/17800"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2019.00098"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.114.250837"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.107.108183"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.93.2.723"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-018-4671-4"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2010-11-2-r14"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.114.123737"}],"container-title":["The Plant Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tpj.16514","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,22]],"date-time":"2024-02-22T16:46:18Z","timestamp":1708620378000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tpj.16514"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,26]]},"references-count":30,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["10.1111\/tpj.16514"],"URL":"https:\/\/doi.org\/10.1111\/tpj.16514","archive":["Portico"],"relation":{},"ISSN":["0960-7412","1365-313X"],"issn-type":[{"value":"0960-7412","type":"print"},{"value":"1365-313X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,26]]},"assertion":[{"value":"2023-09-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}