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Although phytohormones have been implicated in morphological adaptations to water deficits, the molecular mechanisms underlying these processes in woody plants remain unclear. Here, we report that overexpression of <jats:italic>PtoMYB142<\/jats:italic> in <jats:italic>Populus tomentosa<\/jats:italic> results in a dwarfism phenotype with reduced leaf cell size, vessel lumen area, and vessel density in the stem xylem, leading to significantly enhanced drought resistance. We found that PtoMYB142 modulates gibberellin catabolism in response to drought stress by binding directly to the promoter of <jats:italic>PtoGA2ox4<\/jats:italic>, a GA<jats:sub>2<\/jats:sub>\u2010oxidase gene induced under drought stress. Conversely, knockout of <jats:italic>PtoMYB142<\/jats:italic> by the CRISPR\/Cas9 system reduced drought resistance. Our results show that the reduced leaf size and vessel area, as well as the increased vessel density, improve leaf relative water content and stem water potential under drought stress. Furthermore, exogenous GA<jats:sub>3<\/jats:sub> application rescued GA\u2010deficient phenotypes in <jats:italic>PtoMYB142<\/jats:italic>\u2010overexpressing plants and reversed their drought resistance. By suppressing the expression of <jats:italic>PtoGA2ox4<\/jats:italic>, the manifestation of GA\u2010deficient characteristics, as well as the conferred resistance to drought in <jats:italic>PtoMYB142<\/jats:italic>\u2010overexpressing poplars, was impeded. Our study provides insights into the molecular mechanisms underlying tree drought resistance, potentially offering novel transgenic strategies to enhance tree resistance to drought.<\/jats:p>","DOI":"10.1111\/tpj.16588","type":"journal-article","created":{"date-parts":[[2023,12,19]],"date-time":"2023-12-19T14:49:17Z","timestamp":1702997357000},"page":"42-57","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["The transcription factor <scp>PtoMYB142<\/scp> enhances drought tolerance in <i>Populus tomentosa<\/i> by regulating gibberellin catabolism"],"prefix":"10.1111","volume":"118","author":[{"given":"Qin","family":"Song","sequence":"first","affiliation":[{"name":"Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, School of Life Sciences Southwest University  Chongqing 400715 China"},{"name":"Key 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