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Here, we identified a rice TGFS line, <jats:italic>tfs2<\/jats:italic>, through an ethyl methyl sulfone (EMS) mutagenesis strategy. This line showed sterility under high temperature and became fertile under low temperature. Few seeds were produced when the <jats:italic>tfs2<\/jats:italic> stigma was pollinated, indicating that <jats:italic>tfs2<\/jats:italic> is female sterile. Gene cloning and genetic complementation showed that a point mutation from leucine to phenylalanine in HEI10 (HEI10<jats:sup><jats:italic>tfs2<\/jats:italic><\/jats:sup>), a crossover formation protein, caused the TGFS trait of <jats:italic>tfs2<\/jats:italic>. Under high temperature, abnormal univalents were formed, and the chromosomes were unequally segregated during meiosis, similar to the reported meiotic defects in <jats:italic>oshei10<\/jats:italic>. Under low temperature, the number of univalents was largely reduced, and the chromosomes segregated equally, suggesting that crossover formation was restored in <jats:italic>tfs2<\/jats:italic>. Yeast two\u2010hybrid assays showed that HEI10 interacted with two putative protein degradation\u2010related proteins, RPT4 and SRFP1. Through transient expression in tobacco leaves, HEI10 were found to spontaneously aggregate into dot\u2010like foci in the nucleus under high temperature, but HEI10<jats:sup><jats:italic>tfs2<\/jats:italic><\/jats:sup> failed to aggregate. In contrast, low temperature promoted HEI10<jats:sup><jats:italic>tfs2<\/jats:italic><\/jats:sup> aggregation. This result suggests that protein aggregation at the crossover position contributes to the fertility restoration of <jats:italic>tfs2<\/jats:italic> under low temperature. In addition, RPT4 and SRFP1 also aggregated into dot\u2010like foci, and these aggregations depend on the presence of HEI10. These findings reveal a novel mechanism of fertility restoration and facilitate further understanding of HEI10 in meiotic crossover formation.<\/jats:p>","DOI":"10.1111\/tpj.16621","type":"journal-article","created":{"date-parts":[[2024,1,3]],"date-time":"2024-01-03T15:05:05Z","timestamp":1704294305000},"page":"506-518","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["A point mutation in the meiotic crossover formation gene <i>HEI10<\/i>\/<i>TFS2<\/i> leads to thermosensitive genic sterility in rice"],"prefix":"10.1111","volume":"118","author":[{"given":"Zheng","family":"Zhang","sequence":"first","affiliation":[{"name":"Shanghai Engineering Research Center of Plant Germplasm Resources College of Life Sciences, Shanghai Normal University  Shanghai 200234 China"},{"name":"Shanghai Key Laboratory of Plant Molecular Sciences, Shanghai Collaborative Innovation Center of Plant Germplasm Resources Development College of Life Sciences, Shanghai Normal University  Shanghai 200234 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Yi","family":"Guo","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Plant Molecular Sciences, Shanghai Collaborative Innovation Center of Plant Germplasm Resources Development College of Life Sciences, Shanghai Normal University  Shanghai 200234 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Chen","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai Engineering Research Center of Plant Germplasm Resources College of Life Sciences, Shanghai Normal University  Shanghai 200234 China"},{"name":"Shanghai Key Laboratory of Plant Molecular Sciences, Shanghai Collaborative Innovation Center of Plant Germplasm Resources Development College of Life Sciences, Shanghai Normal University  Shanghai 200234 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