{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T15:53:18Z","timestamp":1789573998433,"version":"build-2803163510"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,4,18]],"date-time":"2024-04-18T00:00:00Z","timestamp":1713398400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Thiamine functions as a crucial activator modulating plant health and broad\u2010spectrum stress tolerances. However, the role of thiamine in regulating plant virus infection is largely unknown. Here, we report that the multifunctional 17K protein encoded by barley yellow dwarf virus\u2010GAV (BYDV\u2010GAV) interacted with barley pyrimidine synthase (HvTHIC), a key enzyme in thiamine biosynthesis. HvTHIC was found to be localized in chloroplast via an N\u2010terminal 74\u2010amino acid domain. However, the 17K\u2010HvTHIC interaction restricted HvTHIC targeting to chloroplasts and triggered autophagy\u2010mediated HvTHIC degradation. Upon BYDV\u2010GAV infection, the expression of the <jats:italic>HvTHIC<\/jats:italic> gene was significantly induced, and this was accompanied by accumulation of thiamine and salicylic acid. Silencing of <jats:italic>HvTHIC<\/jats:italic> expression promoted BYDV\u2010GAV accumulation. Transcriptomic analysis of HvTHIC silenced and non\u2010silenced barley plants showed that the differentially expressed genes were mainly involved in plant\u2013pathogen interaction, plant hormone signal induction, phenylpropanoid biosynthesis, starch and sucrose metabolism, photosynthesis\u2010antenna protein, and MAPK signaling pathway. Thiamine treatment enhanced barley resistance to BYDV\u2010GAV. Taken together, our findings reveal a molecular mechanism underlying how BYDV impedes thiamine biosynthesis to uphold viral infection in plants.<\/jats:p>","DOI":"10.1111\/tpj.16772","type":"journal-article","created":{"date-parts":[[2024,4,18]],"date-time":"2024-04-18T16:43:45Z","timestamp":1713458625000},"page":"432-444","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Barley yellow dwarf <scp>virus\u2010GAV 17K<\/scp> protein disrupts thiamine biosynthesis to facilitate viral infection in plants"],"prefix":"10.1111","volume":"119","author":[{"given":"Xiaoyu","family":"Han","sequence":"first","affiliation":[{"name":"College of Plant Protection Henan Agricultural University  Zhengzhou 450046 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Plant Protection Henan Agricultural University  Zhengzhou 450046 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