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Here we demonstrate successful CRISPR\u2010Cas9 genome editing in the dioecious moss species,\n                    <jats:italic>Ceratodon purpureus<\/jats:italic>\n                    . Using an existing selection system from the distantly related hermaphroditic moss,\n                    <jats:italic>Physcomitrium patens<\/jats:italic>\n                    , we generated knock\u2010outs of the\n                    <jats:italic>APT<\/jats:italic>\n                    reporter gene by employing CRISPR\u2010targeted mutagenesis under expression of native U6 snRNA promoters. Next, we used the native homology\u2010directed repair (HDR) pathway, combined with CRISPR\u2010Cas9, to knock in two reporter genes under expression of an endogenous RPS5A promoter in a newly developed landing site in\n                    <jats:italic>C. purpureus<\/jats:italic>\n                    . Our results show that the molecular tools developed in\n                    <jats:italic>P. patens<\/jats:italic>\n                    can be extended to other mosses across this ecologically important and developmentally variable group. These findings pave the way for precise and powerful experiments aimed at identifying the genetic basis of key functional variation within the bryophytes and between the bryophytes and other land plants.\n                  <\/jats:p>","DOI":"10.1111\/tpj.16946","type":"journal-article","created":{"date-parts":[[2024,8,18]],"date-time":"2024-08-18T12:05:08Z","timestamp":1723982708000},"page":"2753-2764","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Establishing\n                    <scp>CRISPR<\/scp>\n                    \u2010Cas9 in the sexually dimorphic moss,\n                    <i>Ceratodon purpureus<\/i>"],"prefix":"10.1111","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3268-1229","authenticated-orcid":false,"given":"Emilie\u2010Katherine","family":"Tavernier","sequence":"first","affiliation":[{"name":"Department of Biology University of Florida  Gainesville Florida USA"},{"name":"Universit\u00e9 Paris\u2010Saclay, INRAE, AgroParisTech, Institut Jean\u2010Pierre Bourgin (IJPB)  Versailles 78000 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7607-3618","authenticated-orcid":false,"given":"Pierre\u2010Fran\u00e7ois","family":"Perroud","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris\u2010Saclay, INRAE, AgroParisTech, Institut Jean\u2010Pierre Bourgin (IJPB)  Versailles 78000 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4484-7081","authenticated-orcid":false,"given":"Emily","family":"Lockwood","sequence":"additional","affiliation":[{"name":"Department of Biology University of Florida  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0619-4638","authenticated-orcid":false,"given":"Fabien","family":"Nogu\u00e9","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris\u2010Saclay, INRAE, AgroParisTech, Institut Jean\u2010Pierre Bourgin (IJPB)  Versailles 78000 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5435-7377","authenticated-orcid":false,"given":"Stuart F.","family":"McDaniel","sequence":"additional","affiliation":[{"name":"Department of Biology University of Florida  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.16.00196"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13579"},{"key":"e_1_2_9_4_1","doi-asserted-by":"crossref","unstructured":"Bechteler J. 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