{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T02:36:02Z","timestamp":1791426962789,"version":"4.3.3"},"reference-count":68,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,8,21]],"date-time":"2024-08-21T00:00:00Z","timestamp":1724198400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFD2200201"],"award-info":[{"award-number":["2023YFD2200201"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>As a dynamic and reversible post\u2010transcriptional marker, <jats:italic>N<\/jats:italic><jats:sup>6<\/jats:sup>\u2010methyladenosine (m<jats:sup>6<\/jats:sup>A) plays an important role in the regulation of biological functions, which are mediated by m<jats:sup>6<\/jats:sup>A pathway components including writers (MT\u2010A70, FIP37, VIR and HAKAI family), erasers (ALKBH family) and readers (YTH family). There is an urgent need for a comprehensive analysis of m<jats:sup>6<\/jats:sup>A pathway components across species at evolutionary levels. In this study, we identified 4062 m<jats:sup>6<\/jats:sup>A pathway components from 154 plant species including green algae, utilizing large\u2010scale phylogenetic to explore their origin and evolution. We discovered that the copy number of writers was conserved among different plant lineages, with notable expansions in the ALKBH and YTH families. Synteny network analysis revealed conserved genomic contexts and lineage\u2010specific transpositions. Furthermore, we used Direct RNA Sequencing (DRS) to reveal the Poly(A) length (PAL) and m<jats:sup>6<\/jats:sup>A ratio profiles in six angiosperms species, with a particular focus on the m<jats:sup>6<\/jats:sup>A pathway components. The ECT1\/2\u2010Poeaece4 sub\u2010branches (YTH family) with unique genomic contexts exhibited significantly higher expression level than genes of other ECT1\/2 poeaece sub\u2010branches (ECT1\/2\u2010Poeaece1\u20103), accompanied by lower m<jats:sup>6<\/jats:sup>A modification and PAL. Besides, conserved m<jats:sup>6<\/jats:sup>A sites distributed in CDS and 3\u2032UTR were detected in the ECT1\/2\u2010Poaceae4, and the dual\u2010luciferase assay further demonstrated that these conserved m<jats:sup>6<\/jats:sup>A sites in the 3\u2032UTR negatively regulated the expression of Firefly luciferase (LUC) gene. Finally, we developed transcription factor regulatory networks for m<jats:sup>6<\/jats:sup>A pathway components, using yeast one\u2010hybrid assay demonstrated that <jats:italic>PheBPC1<\/jats:italic> could interact with the <jats:italic>PheECT1\/2\u20105<\/jats:italic> promoter. Overall, this study presents a comprehensive evolutionary and functional analysis of m<jats:sup>6<\/jats:sup>A pathway components and their modifications in plants, providing a valuable resource for future functional analysis in this field.<\/jats:p>","DOI":"10.1111\/tpj.16996","type":"journal-article","created":{"date-parts":[[2024,8,21]],"date-time":"2024-08-21T22:44:04Z","timestamp":1724280244000},"page":"505-525","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Evolution and post\u2010transcriptional regulation insights of m<sup>6<\/sup>A writers, erasers, and readers in plant epitranscriptome"],"prefix":"10.1111","volume":"120","author":[{"given":"Jun","family":"Zhang","sequence":"first","affiliation":[{"name":"Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, College of Life Science Fujian Agriculture and Forestry University  Fuzhou 350002 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350002 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1515-9463","authenticated-orcid":false,"given":"Huiyuan","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Forestry Fujian Agriculture and Forestry University  Fuzhou 350002 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangrong","family":"Li","sequence":"additional","affiliation":[{"name":"Basic Forestry and Proteomics Research Center, School of Future Technology Fujian Agriculture and Forestry University  Fuzhou 350002 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liangzhen","family":"Zhao","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, College of Life Science Fujian Agriculture and Forestry University  Fuzhou 350002 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