{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T15:22:22Z","timestamp":1780068142014,"version":"3.54.0"},"reference-count":81,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,10,20]],"date-time":"2024-10-20T00:00:00Z","timestamp":1729382400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFD1400200"],"award-info":[{"award-number":["2022YFD1400200"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Pustule formation is pivotal for the development of the <jats:italic>Xanthomonas citri<\/jats:italic> subsp. <jats:italic>citri<\/jats:italic> (Xcc)\u2010induced citrus canker disease (CCD). Although our previous study demonstrated that the exogenous application of abscisic acid (ABA) facilitated pustule formation induced by Xcc, the precise mechanism remains elusive. The 9\u2010cis\u2010epoxycarotenoid dioxygenase (NCED) is a crucial enzyme in ABA biosynthesis. This study explored the role of citrus <jats:italic>CsNCED1\u20101<\/jats:italic> in CCD resistance through overexpression and RNA interference of <jats:italic>CsNCED1\u20101<\/jats:italic> in Wanjincheng orange (<jats:italic>Citrus sinensis<\/jats:italic>). Our findings indicated that <jats:italic>CsNCED1\u20101<\/jats:italic> negatively modulated CCD resistance by fostering ABA accumulation, concomitant with an increase in jasmonic acid (JA) and a decrease in salicylic acid (SA). Plants overexpressing <jats:italic>CsNCED1\u20101<\/jats:italic> displayed shortened leaves with smaller and denser stomata along with irregular and increased palisade cells. <jats:italic>CsLOB1<\/jats:italic> is a known susceptibility gene for CCD, and <jats:italic>CsbZIP40<\/jats:italic> positively influences resistance to this disease. We further confirmed that CsLOB1 promoted and CsbZIP40 suppressed the transcription of <jats:italic>CsNCED1\u20101<\/jats:italic> by directly binding to the <jats:italic>CsNCED1\u20101<\/jats:italic> promoter. Notably, CsbZIP40 and CsLOB1 showed a competitive relationship in the regulation of <jats:italic>CsNCED1\u20101<\/jats:italic> expression, with CsbZIP40 exhibiting greater competitiveness. Overall, our findings highlight that <jats:italic>CsNCED1\u20101<\/jats:italic> promotes susceptibility to citrus canker by disrupting JA\u2010 and SA\u2010mediated defense mechanisms and triggering the proliferation and remodeling of palisade cells, thereby facilitating pathogen colonization and pustule formation. This study offers novel insights into the regulatory mechanisms underlying citrus canker resistance and the role of <jats:italic>CsNCED1\u20101<\/jats:italic> in citrus.<\/jats:p>","DOI":"10.1111\/tpj.17075","type":"journal-article","created":{"date-parts":[[2024,10,20]],"date-time":"2024-10-20T17:18:57Z","timestamp":1729444737000},"page":"1625-1642","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Competitive control of <i>CsNCED1\u20101<\/i> by CsLOB1 and CsbZIP40 triggers susceptibility to citrus canker"],"prefix":"10.1111","volume":"120","author":[{"given":"Qin","family":"Long","sequence":"first","affiliation":[{"name":"Citrus Research Institute Southwest University  Chongqing 400712 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lehuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Citrus Research Institute Southwest University  Chongqing 400712 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianxiang","family":"Zhu","sequence":"additional","affiliation":[{"name":"Citrus Research Institute Southwest University  Chongqing 400712 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuyang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Citrus Research Institute Southwest University  Chongqing 400712 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changyu","family":"Zou","sequence":"additional","affiliation":[{"name":"Citrus Research Institute Southwest University  Chongqing 400712 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanzhen","family":"Xu","sequence":"additional","affiliation":[{"name":"Citrus Research Institute Southwest University  Chongqing 400712 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongrui","family":"He","sequence":"additional","affiliation":[{"name":"Citrus Research Institute Southwest University  Chongqing 400712 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanchun","family":"Chen","sequence":"additional","affiliation":[{"name":"Citrus Research Institute Southwest University  Chongqing 400712 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7522-0444","authenticated-orcid":false,"given":"Xiuping","family":"Zou","sequence":"additional","affiliation":[{"name":"Citrus Research Institute Southwest University  Chongqing 400712 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,20]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/03235408.2016.1143573"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.104.025833"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt997"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01933993"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.107.099226"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.010605"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2017.08.011"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.111.192641"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1071\/FP09048"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1364-3703.2003.00163.x"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.14425"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2009.05.006"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1364-3703.2008.00486.x"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hpj.2016.10.001"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1998.tb12863.x"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2009.03875.x"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601575"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.12667"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI.1999.12.6.556"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envexpbot.2021.104404"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-21-3-0361"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jobm.201200311"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0810107105"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1094\/PHP-2002-0812-01-RV"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1364-3703.2004.00197.x"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-06-12-0164-R"},{"issue":"1","key":"e_1_2_10_28_1","first-page":"21","article-title":"Visualization of protein interactions in living cells using bimolecular fluorescence complementation (BiFC) analysis","volume":"29","author":"Hu C.D.","year":"2006","journal-title":"Current Protocols in Protein Science"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2022.02.002"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1313271111"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2018.00162"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm775"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2009.09.014"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313x.2001.01096.x"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.16800"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2009.04.005"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-20397-3_5"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0223498"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/mp\/sst176"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-018-0768-z"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41438-021-00486-2"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2019.01634"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.14196"},{"issue":"5","key":"e_1_2_10_45_1","first-page":"969","article-title":"Plant stomata function in innate immunity against bacterial invasion","volume":"126","author":"Melotto M.","year":"2006","journal-title":"The Plant Cell"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10142-006-0041-4"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001513"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.56.032604.144046"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-06531-5"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.106.079475"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envexpbot.2022.104885"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.12733"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1911660116"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-15-157"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.164"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.26.15354"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.010663"},{"key":"e_1_2_10_58_1","first-page":"8","article-title":"Roles of a sustained activation of NCED3 and the synergistic regulation of ABA biosynthesis and catabolism in ABA signal production in Arabidopsis","volume":"52","author":"Ren H.","year":"2007","journal-title":"Science Bulletin"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erj048"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2022.02.006"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-023-00999-8"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.104.046169"},{"key":"e_1_2_10_63_1","first-page":"1082","article-title":"Crucial roles of abscisic acid biogenesis in virulence of rice blast fungus Magnaporthe oryzae","volume":"6","author":"Spence C.A.","year":"2015","journal-title":"Plant Science"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/345783a0"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.111.186866"},{"key":"e_1_2_10_66_1","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1111\/pbi.14219","article-title":"The transcriptional regulatory module CsHB5\u2010CsbZIP44 positively regulates abscisic acid\u2010mediated carotenoid biosynthesis in citrus (Citrus spp.)","volume":"22","author":"Sun Q.","year":"2023","journal-title":"Plant Biotechnology Journal"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-81-802"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.2003.01786.x"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008886"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.1890\/02-0710"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2009.03.006"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2004.02028.x"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00938.x"},{"key":"e_1_2_10_74_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2017.00782"},{"key":"e_1_2_10_75_1","doi-asserted-by":"publisher","DOI":"10.1093\/aob\/mcm079"},{"key":"e_1_2_10_76_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiad336"},{"key":"e_1_2_10_77_1","doi-asserted-by":"publisher","DOI":"10.1093\/hr\/uhad276"},{"key":"e_1_2_10_78_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.15431"},{"key":"e_1_2_10_79_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.12743"},{"key":"e_1_2_10_80_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.14684"},{"key":"e_1_2_10_81_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.15217"},{"key":"e_1_2_10_82_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11240-013-0423-y"}],"container-title":["The Plant Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tpj.17075","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,18]],"date-time":"2024-11-18T04:58:10Z","timestamp":1731905890000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tpj.17075"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,20]]},"references-count":81,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,11]]}},"alternative-id":["10.1111\/tpj.17075"],"URL":"https:\/\/doi.org\/10.1111\/tpj.17075","archive":["Portico"],"relation":{},"ISSN":["0960-7412","1365-313X"],"issn-type":[{"value":"0960-7412","type":"print"},{"value":"1365-313X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,20]]},"assertion":[{"value":"2024-01-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}