{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:10:53Z","timestamp":1787238653653,"version":"build-2736575974"},"reference-count":73,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T00:00:00Z","timestamp":1733356800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T00:00:00Z","timestamp":1733356800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["#31125022"],"award-info":[{"award-number":["#31125022"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003512","name":"State Administration of Foreign Experts Affairs","doi-asserted-by":"publisher","award":["B18042"],"award-info":[{"award-number":["B18042"]}],"id":[{"id":"10.13039\/501100003512","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    Small RNAs are involved in diverse cellular processes, including plant immunity to pathogens. Here, we report that miR158a negatively regulates plant immunity to the oomycete pathogen\n                    <jats:italic>Phytophthora parasitica<\/jats:italic>\n                    in\n                    <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                    . By performing real\u2010time quantitative PCR, transient expression, and RNA ligase\u2010mediated 5\u2032 rapid amplification of cDNA ends assays, we demonstrate that miR158a downregulates\n                    <jats:italic>AtTN7<\/jats:italic>\n                    expression by cleaving its 3\u2032\u2010untranslated region.\n                    <jats:italic>AtTN7<\/jats:italic>\n                    positively affects plant immunity and encodes a truncated intracellular nucleotide\u2010binding site and leucine\u2010rich repeat receptor containing the Toll\/interleukin\u20101 receptor. AtTN7 can degrade oxidized forms of nicotinamide adenine dinucleotide (NAD+). Further genetic and molecular analyses reveal that the Enhanced Disease Susceptibility 1\u2010Phytoalexin Deficient 4\u2010Activated Disease Resistance 1 complex is required for AtTN7\u2010mediated immunity. ADR1\u2010dependent Ca\n                    <jats:sup>2+<\/jats:sup>\n                    influx is crucial for activating salicylic acid signaling to condition AtTN7\u2010triggered immunity. Our study uncovers the immune roles and regulatory mechanisms of miR158a and its target\n                    <jats:italic>AtTN7<\/jats:italic>\n                    . Both miR158a\u2010downregulation and\n                    <jats:italic>AtTN7<\/jats:italic>\n                    \u2010overexpression lead to enhanced plant resistance to\n                    <jats:italic>P. parasitica<\/jats:italic>\n                    without affecting plant growth phenotypes, suggesting their application potentials and the utilization of miRNAs in identifying novel immune genes for the development of plant germplasm resources with enhanced disease resistance.\n                  <\/jats:p>","DOI":"10.1111\/tpj.17194","type":"journal-article","created":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T11:49:53Z","timestamp":1733399393000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<scp>miR158a<\/scp>\n                    negatively regulates plant resistance to\n                    <i>Phytophthora parasitica<\/i>\n                    by repressing\n                    <scp>AtTN7<\/scp>\n                    that requires\n                    <scp>EDS1<\/scp>\n                    \u2010\n                    <scp>PAD4<\/scp>\n                    \u2010\n                    <scp>ADR1<\/scp>\n                    complex in\n                    <i>Arabidopsis thaliana<\/i>"],"prefix":"10.1111","volume":"121","author":[{"given":"Yilin","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Crop Stress Resistance and High\u2010Efficiency Production, and College of Agronomy Northwest A&amp;F University  Yangling Shaanxi 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuhong","family":"Gou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Stress Resistance and High\u2010Efficiency Production, and College of Plant Protection Northwest A&amp;F University  Yangling Shaanxi 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruize","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Stress Resistance and High\u2010Efficiency Production, and College of Agronomy Northwest A&amp;F University  Yangling Shaanxi 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peiling","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Stress Resistance and High\u2010Efficiency Production, and College of Agronomy Northwest A&amp;F University  Yangling Shaanxi 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Assiya","family":"Ansabayeva","sequence":"additional","affiliation":[{"name":"Department of Agronomy A. Baitursynov Kostanay Regional University  Kostanay 110000 Republic of Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingyao","family":"Shi","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Stress Resistance and High\u2010Efficiency Production, and College of Agronomy Northwest A&amp;F University  Yangling Shaanxi 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeming","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Stress Resistance and High\u2010Efficiency Production, and College of Agronomy Northwest A&amp;F University  Yangling Shaanxi 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuling","family":"Meng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Stress Resistance and High\u2010Efficiency Production, and College of Agronomy Northwest A&amp;F University  Yangling Shaanxi 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7286-4041","authenticated-orcid":false,"given":"Weixing","family":"Shan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Stress Resistance and High\u2010Efficiency Production, and College of Agronomy Northwest A&amp;F University  Yangling Shaanxi 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2023.03.022"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.ade8487"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2018.01038"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.15659"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22084004"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.14302"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2017.04.015"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr319"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2021.102039"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07612"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2001185117"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abo0001"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000219"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2020.04.006"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1094\/mpmi\u201009\u201015\u20100212\u2010fi"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/embor.2008.247"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1042\/bst0350109"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.15792"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aax1911"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.107.050062"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.12760"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abq3297"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19020515"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abg7917"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abq8180"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.12190"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010020\u201020777\u2010x"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1094\/mpmi\u201006\u201022\u20100133\u2010r"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/plcell\/koac035"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.13176"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.12883"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2015.01.014"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2023.111752"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erad268"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.12971"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425\u2010017\u20102773\u2010x"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abe3069"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103\u2010016\u20100563\u20107"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abd9993"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00251\u2010021\u201001242\u20105"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u2010313x.2002.01404.x"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.113.219162"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21217986"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.784484"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.13707"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.113.227686"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.13717"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000783"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.105.039834"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13205\u2010021\u201002810\u2010y"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xplc.2021.100180"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010024\u201007183\u20109"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes13010110"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms10159"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201023614\u2010x"},{"key":"e_1_2_9_57_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C.(2019)Identification and functional characterization of Arabidopsis small RNAs in response to Phytophthora infection(PhD thesis). Yangling: The Northwest A&F University."}],"container-title":["The Plant Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tpj.17194","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/tpj.17194","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tpj.17194","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T12:41:47Z","timestamp":1782218507000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tpj.17194"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,5]]},"references-count":73,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1111\/tpj.17194"],"URL":"https:\/\/doi.org\/10.1111\/tpj.17194","archive":["Portico"],"relation":{},"ISSN":["0960-7412","1365-313X"],"issn-type":[{"value":"0960-7412","type":"print"},{"value":"1365-313X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,5]]},"assertion":[{"value":"2024-08-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e17194"}}