{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T04:41:21Z","timestamp":1788583281487,"version":"build-2803163510"},"reference-count":96,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T00:00:00Z","timestamp":1739923200000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31500209"],"award-info":[{"award-number":["31500209"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    Salicylic acid (SA) plays important roles in plant local and systemic resistance. Isochorismate synthase 1 (ICS1) is a key enzyme in SA synthesis. Pathogens infection triggered the\n                    <jats:italic>ICS1<\/jats:italic>\n                    expression and induced SA production. However, the molecular regulation mechanism of\n                    <jats:italic>ICS1<\/jats:italic>\n                    against virus infection remains unclear. Here, we employed molecular genetics and physiobiochemical approaches to confirm a transcription factor\n                    <jats:italic>NbNAC1<\/jats:italic>\n                    from\n                    <jats:italic>Nicotiana benthamiana<\/jats:italic>\n                    is a positive regulator of resistance against tobacco mosaic virus (TMV). The pathways\n                    <jats:italic>NbNAC1<\/jats:italic>\n                    and\n                    <jats:italic>NbICS1<\/jats:italic>\n                    can be triggered by TMV infection. Silencing\n                    <jats:italic>NbNAC1<\/jats:italic>\n                    accelerated TMV\u2010induced oxidative damage and increased reactive oxygen species (ROS) production. It also weakened both local and systemic resistance against TMV and decreased the expression of\n                    <jats:italic>NbICS1<\/jats:italic>\n                    , SA signaling gene\n                    <jats:italic>NbNPR1<\/jats:italic>\n                    , and SA defense\u2010related genes. The effects of\n                    <jats:italic>NbNAC1<\/jats:italic>\n                    \u2010silencing were restored by overexpression of\n                    <jats:italic>NbICS1<\/jats:italic>\n                    or foliar SA applications. Overexpressing\n                    <jats:italic>NbNAC1<\/jats:italic>\n                    prevented oxidative damage and reduced the production of ROS, enhanced plant resistance against viral pathogen, and activated\n                    <jats:italic>NbICS1<\/jats:italic>\n                    expression, and SA downstream signaling and defense\u2010related genes. NbNAC1 localized in nuclear and emerged the ability of transcriptional regulation. ChIP and EMSA results indicated that NbNAC1 directly binds to a fragment containing GAAATT motif of\n                    <jats:italic>NbICS1<\/jats:italic>\n                    promoter. Luciferase reporter assays confirmed that\n                    <jats:italic>NbNAC1<\/jats:italic>\n                    activates\n                    <jats:italic>NbICS1<\/jats:italic>\n                    expression. Taken together, our results demonstrate that\n                    <jats:italic>NbNAC1<\/jats:italic>\n                    plays a critical role in plant immunity through activation of SA production.\n                  <\/jats:p>","DOI":"10.1111\/tpj.17242","type":"journal-article","created":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T04:24:18Z","timestamp":1739939058000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["<i>NbNAC1<\/i>\n                    enhances plant immunity against TMV by regulating isochorismate synthase 1 expression and the SA pathway"],"prefix":"10.1111","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6853-7291","authenticated-orcid":false,"given":"Feng","family":"Zhu","sequence":"first","affiliation":[{"name":"College of Plant Protection Yangzhou University  Yangzhou 225009 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Biology Shanghai Jiao Tong University  Shanghai 200240 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maha","family":"AlKhazindar","sequence":"additional","affiliation":[{"name":"Botany and Microbiology Department, Faculty of Science Cairo University  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaolin","family":"Ji","sequence":"additional","affiliation":[{"name":"College of Plant Protection Yangzhou University  Yangzhou 225009 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