{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T19:37:56Z","timestamp":1791229076325,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T00:00:00Z","timestamp":1738800000000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001843","name":"Science and Engineering Research Board","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Glycerophosphodiester phosphodiesterases (GDPDs) enzymes are known to be involved in phospholipids degradation pathways, where glycerophosphodiesters are hydrolyzed to glycerol\u20103\u2010phosphate (G3P) and corresponding alcohol. In plants, GDPDs are involved in phosphate deficiency adaptive responses and have been shown to impact root length, but the precise mechanism remains unclear. This study focuses on the rice <jats:italic>GDPD5<\/jats:italic> gene and its role in regulating primary root growth. Our research demonstrates that <jats:italic>OsGDPD5<\/jats:italic> encodes a functional GDPD enzyme and could hydrolyze glycerophosphocholine and glycerophosphorylethanolamine. At transcriptional levels, <jats:italic>OsGDPD5<\/jats:italic> is preferentially expressed in the root tip and regulated by transcription factor OsPHR2. We have used CRISPR\/Cas9 to generate <jats:italic>OsGDPD5<\/jats:italic> knock\u2010out lines, allowing us to explore its role in root growth. Our findings show that <jats:italic>osgdpd5<\/jats:italic> mutants had a shorter primary root, which could be restored to a normal level by the exogenous application of sugar or G3P. Further, knocking out <jats:italic>OsGDPD5<\/jats:italic> alters endogenous levels of G3P and sugars, affecting auxin biosynthesis in the root and, ultimately, primary root growth. In this manner, OsGDPD5 has a crucial role in regulating physiological processes, specifically sugar and auxin signaling, which are known to be involved in root growth regulation in rice. Our research thus unraveled a link between rice phosphate deficiency\u2010responsive lipid remodeling and root growth via sugar\u2010hormone signaling.<\/jats:p>","DOI":"10.1111\/tpj.17249","type":"journal-article","created":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T10:10:01Z","timestamp":1738836601000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Phosphate deficiency inducible <scp><i>OsGDPD5<\/i><\/scp> affects root growth by regulating sugar\u2010auxin crosstalk"],"prefix":"10.1111","volume":"121","author":[{"given":"Lokesh","family":"Verma","sequence":"first","affiliation":[{"name":"National Institute of Plant Genome Research  Aruna Asaf Ali Marg New Delhi 110067 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mandavi","family":"Pandey","sequence":"additional","affiliation":[{"name":"National Institute of Plant Genome Research  Aruna Asaf Ali Marg New Delhi 110067 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chitra","family":"Bhatia","sequence":"additional","affiliation":[{"name":"National Institute of Plant Genome Research  Aruna Asaf Ali Marg New Delhi 110067 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Poonam","family":"Mehra","sequence":"additional","affiliation":[{"name":"National Institute of Plant Genome Research  Aruna Asaf Ali Marg New Delhi 110067 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhagat","family":"Singh","sequence":"additional","affiliation":[{"name":"National Institute of Plant Genome Research  Aruna Asaf Ali Marg New Delhi 110067 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6969-5187","authenticated-orcid":false,"given":"Jitender","family":"Giri","sequence":"additional","affiliation":[{"name":"National Institute of Plant Genome Research  Aruna Asaf Ali Marg New Delhi 110067 India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcad013"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-11490-9"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.111.173724"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2011.04538.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.12699"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40502-019-00462-y"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/eraa482"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01101680"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.22.6717-6720.2001"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.112.207738"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erm221"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10930-024-10190-4"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/plcell\/koae041"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1987.tb02730.x"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erv025"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-005-2159-5"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/plb.13468"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2022.01.016"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.112.205575"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.13090"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.001735"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2009.02.011"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00299-016-1984-0"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.12699"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.13459"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0004502"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2018.10.018"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erac064"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01223-07"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/eru534"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.16463"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.4252"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.17.00571"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-021-03657-6"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiae453"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiac586"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12298-023-01319-3"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1201\/9781003440079-8"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.112.104794"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/aobpla\/pls047"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2006.02836.x"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2017.04.006"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erac192"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40502-021-00624-x"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cj.2020.05.004"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2018.04.002"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1420294112"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj0570508"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants11172238"}],"container-title":["The Plant Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tpj.17249","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T07:29:32Z","timestamp":1743751772000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tpj.17249"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2]]},"references-count":49,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,2]]}},"alternative-id":["10.1111\/tpj.17249"],"URL":"https:\/\/doi.org\/10.1111\/tpj.17249","archive":["Portico"],"relation":{},"ISSN":["0960-7412","1365-313X"],"issn-type":[{"value":"0960-7412","type":"print"},{"value":"1365-313X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2]]},"assertion":[{"value":"2024-08-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e17249"}}