{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T03:21:00Z","timestamp":1791688860418,"version":"4.3.4"},"reference-count":53,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,2,24]],"date-time":"2025-02-24T00:00:00Z","timestamp":1740355200000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFD2300700"],"award-info":[{"award-number":["2023YFD2300700"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31972479"],"award-info":[{"award-number":["31972479"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017367","name":"Research Fund for Young Star of Science and Technology in Shaanxi Province","doi-asserted-by":"publisher","award":["2022KJXX\u201080"],"award-info":[{"award-number":["2022KJXX\u201080"]}],"id":[{"id":"10.13039\/100017367","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2025,2]]},"abstract":"SUMMARY<\/jats:title>\n \n Melatonin is a pivotal bioactive molecule that enhances plant cold stress tolerance, but the precise mechanisms remain enigmatic. Here, we have discovered that overexpressing melatonin biosynthetic gene\n ClCOMT1<\/jats:italic>\n or applying exogenous melatonin activates the C\u2010repeat binding factor (CBF)\u2010responsive pathway and enhances watermelon cold tolerance. This enhancement is accompanied by elevated levels of nitric oxide (NO) and hydrogen sulfide (H\n 2<\/jats:sub>\n S), along with upregulation of\n nitrate reductase 1<\/jats:italic>\n (\n ClNR1<\/jats:italic>\n ) and\n L\u2010cysteine desulfhydrase<\/jats:italic>\n (\n ClLCD<\/jats:italic>\n ) genes involved in NO and H\n 2<\/jats:sub>\n S generation respectively. Conversely, knockout of\n ClCOMT1<\/jats:italic>\n exhibits contrasting effects compared to its overexpression. Furthermore, application of sodium nitroprusside (SNP, a NO donor) and NaHS (a H\n 2<\/jats:sub>\n S donor) promotes the accumulation of H\n 2<\/jats:sub>\n S and NO, respectively, activating the CBF pathway and enhancing cold tolerance. However, knockout of\n ClNR1<\/jats:italic>\n or\n ClLCD<\/jats:italic>\n abolished melatonin\u2010induced H\n 2<\/jats:sub>\n S or NO production respectively and abrogated melatonin\u2010induced CBF pathway and cold tolerance. Conversely, supplementation with SNP and NaHS restored the diminished cold response caused by\n ClCOMT1<\/jats:italic>\n deletion. Additionally, deletion of either\n ClNR1<\/jats:italic>\n or\n ClLCD<\/jats:italic>\n eliminated NaHS\u2010 or SNP\u2010induced cold response, respectively. Overall, these findings suggest a reciprocal positive\u2010regulatory loop between\n ClNR1<\/jats:italic>\n \u2010mediated NO and\n ClLCD<\/jats:italic>\n \u2010mediated H\n 2<\/jats:sub>\n S, which plays a crucial role in mediating the melatonin\u2010induced enhancement of cold tolerance.\n <\/jats:p>","DOI":"10.1111\/tpj.70025","type":"journal-article","created":{"date-parts":[[2025,2,26]],"date-time":"2025-02-26T14:17:14Z","timestamp":1740579434000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["A self\u2010amplifying\n NO<\/scp>\n \u2010\n \n H\n 2<\/sub>\n S\n <\/scp>\n loop mediates melatonin\u2010induced\n CBF<\/scp>\n \u2010responsive pathway and cold tolerance in watermelon"],"prefix":"10.1111","volume":"121","author":[{"given":"Yanliang","family":"Guo","sequence":"first","affiliation":[{"name":"State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture Northwest A&F University 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