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While using HLP tobacco as a sustainable fuel source is promising, it has not yet been tested for its tolerance to warmer environments that are expected in the near future as a result of climate change. We found that HLP tobacco had reduced stomatal conductance, which results in increased leaf temperatures up to 1.5\u00b0C higher under control and high temperature (38\u00b0C day\/28\u00b0C night) conditions, reduced transpiration, and reduced CO\n                    <jats:sub>2<\/jats:sub>\n                    assimilation. We hypothesize this reduction in stomatal conductance is due to the presence of excessive, large lipid droplets in HLP guard cells imaged using confocal microscopy. High temperatures also significantly reduced total fatty acid levels by 55% in HLP plants; thus, additional engineering may be needed to maintain high titers of leaf oil under future climate conditions. High\u2010throughput image analysis techniques using open\u2010source image analysis platform PlantCV for thermal image analysis (plant temperature), stomata microscopy image analysis (stomatal conductance), and fluorescence image analysis (photosynthetic efficiency) were developed and applied in this study. A corresponding set of PlantCV tutorials are provided to enable similar studies focused on phenotyping future crops under adverse conditions.\n                  <\/jats:p>","DOI":"10.1111\/tpj.70067","type":"journal-article","created":{"date-parts":[[2025,3,16]],"date-time":"2025-03-16T01:13:37Z","timestamp":1742087617000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Excessive leaf oil modulates the plant abiotic stress response via reduced stomatal aperture in tobacco (\n                    <i>Nicotiana tabacum<\/i>\n                    )"],"prefix":"10.1111","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8891-4274","authenticated-orcid":false,"given":"Katherine M.","family":"Murphy","sequence":"first","affiliation":[{"name":"Donald Danforth Plant Science Center  975 N. 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