{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T14:18:57Z","timestamp":1785161937525,"version":"3.55.0"},"reference-count":76,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T00:00:00Z","timestamp":1745884800000},"content-version":"vor","delay-in-days":28,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    Roots play a critical role in the sensing and absorption of essential minerals from the rhizosphere. Iron (Fe) deficiency, for example, triggers a well\u2010known series of physiological and molecular responses within roots that facilitate uptake, which differs between monocots and dicots. In monocots, little is known about the molecular responses that occur within specific root development zones in response to iron deprivation, and how these differences result in overall nutrient uptake. Here, we conducted a transcriptome analysis of wheat root tips under Fe deficiency (\u2212Fe) and performed a comparative transcriptome analysis with the previous datasets generated from the whole root. Gene ontology analysis of differentially expressed genes highlighted the significance of oxidoreductase activity and metal\/ion transport in the root tip, which are critical for Fe mobilization. Interestingly, wheat, an allohexaploid species consisting of three different genomes (A, B, and D) displayed varying gene expression levels arising from the three genomes that contributed to similar molecular functions. Detailed analysis of oxidoreductase function at the root tip revealed multiple\n                    <jats:italic>m<\/jats:italic>\n                    ulti\n                    <jats:italic>c<\/jats:italic>\n                    opper\n                    <jats:italic>o<\/jats:italic>\n                    xidase (MCO) proteins, such as Fe\u2010responsive TaMCO3, that likely contribute to the overall ferroxidase activity. Further characterization of\n                    <jats:italic>TaMCO3<\/jats:italic>\n                    shows that it complements the yeast FET3 mutant and rescues the \u2212Fe sensitivity phenotype of Arabidopsis\n                    <jats:italic>atmco3<\/jats:italic>\n                    mutants by enhancing vascular Fe loading. Transgenic wheat lines overexpressing TaMCO3 exhibited increased root Fe accumulation and improved tolerance to \u2212Fe by augmenting the expression of Fe\u2010mobilizing genes. Our findings highlight the role of spatially resolved gene expression in \u2212Fe responses, suggesting strategies to reprogram cells for improved nutrient stress tolerance.\n                  <\/jats:p>","DOI":"10.1111\/tpj.70188","type":"journal-article","created":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T08:57:49Z","timestamp":1745917069000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Integrative spatial transcriptomic analysis pinpoints the role of the ferroxidase,\n                    <scp>TaMCO3<\/scp>\n                    , in wheat root tip iron mobilization"],"prefix":"10.1111","volume":"122","author":[{"given":"Riya","family":"Joon","sequence":"first","affiliation":[{"name":"National Agri\u2010Food Biotechnology Institute (Department of Biotechnology)  Sector 81, Knowledge City, S.A.S. Nagar Mohali Punjab India"},{"name":"Department of Biotechnology Panjab University  Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gourav","family":"Singh","sequence":"additional","affiliation":[{"name":"National Agri\u2010Food Biotechnology Institute (Department of Biotechnology)  Sector 81, Knowledge City, S.A.S. Nagar Mohali Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepshikha","family":"Tyagi","sequence":"additional","affiliation":[{"name":"National Agri\u2010Food Biotechnology Institute (Department of Biotechnology)  Sector 81, Knowledge City, S.A.S. Nagar Mohali Punjab India"},{"name":"Regional Centre of Biotechnology  Faridabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Varsha","family":"Meena","sequence":"additional","affiliation":[{"name":"National Agri\u2010Food Biotechnology Institute (Department of Biotechnology)  Sector 81, Knowledge City, S.A.S. Nagar Mohali Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vishnu","family":"Shukla","sequence":"additional","affiliation":[{"name":"Indian Institute of Science Education and Research (IISER)  Tirupati Andhra Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanupriya","family":"Agrwal","sequence":"additional","affiliation":[{"name":"National Agri\u2010Food Biotechnology Institute (Department of Biotechnology)  Sector 81, Knowledge City, S.A.S. Nagar Mohali Punjab India"},{"name":"Department of Biotechnology Panjab University  Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shivani","family":"Saini","sequence":"additional","affiliation":[{"name":"National Agri\u2010Food Biotechnology Institute (Department of Biotechnology)  Sector 81, Knowledge City, S.A.S. Nagar Mohali Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Mankiran","sequence":"additional","affiliation":[{"name":"National Agri\u2010Food Biotechnology Institute (Department of Biotechnology)  Sector 81, Knowledge City, S.A.S. Nagar Mohali Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamida","family":"Banoo","sequence":"additional","affiliation":[{"name":"National Agri\u2010Food Biotechnology Institute (Department of Biotechnology)  Sector 81, Knowledge City, S.A.S. Nagar Mohali Punjab India"},{"name":"Regional Centre of Biotechnology  Faridabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santosh B.","family":"Satbhai","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Indian Institute of Science Education and Research (IISER)  Mohali India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jagtar","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Panjab University  Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terri","family":"Long","sequence":"additional","affiliation":[{"name":"Department of Plant and Microbial Biology North Carolina State University  Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eswarayya","family":"Ramireddy","sequence":"additional","affiliation":[{"name":"Indian Institute of Science Education and Research (IISER)  Tirupati Andhra Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1064-139X","authenticated-orcid":false,"given":"Ajay K.","family":"Pandey","sequence":"additional","affiliation":[{"name":"National Agri\u2010Food Biotechnology Institute (Department of Biotechnology)  Sector 81, Knowledge City, S.A.S. 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