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While studies in the model plant <jats:italic>Arabidopsis thaliana<\/jats:italic> have significantly advanced our understanding of circadian function, recent research has also focused on crop species for improved yield and quality. In this study, we examined the rhythmic behavior and regulatory function of circadian clock components in tomato (<jats:italic>Solanum lycopersicum<\/jats:italic>). Time course analyses of gene expression over the circadian cycle revealed robust rhythmic oscillations in tomato leaves under free\u2010running conditions. Comparative analyses showed similar peak phases for several clock genes in <jats:italic>Arabidopsis<\/jats:italic> and tomato, suggesting functional conservation. Rhythms in tomato fruits, however, showed reduced amplitude, slight phase changes, or arrhythmia, indicating organ\u2010specific circadian variations. By using <jats:italic>CRISPR\u2010Cas9<\/jats:italic> gene editing strategies (<jats:italic>clock<\/jats:italic><jats:sup><jats:italic>crispr<\/jats:italic><\/jats:sup>), we also showed that proper clock gene expression is essential for setting the phase in tomato plants. Leaf movement analyses also showed a phase change in the <jats:italic>clock<\/jats:italic><jats:sup><jats:italic>crispr<\/jats:italic><\/jats:sup> lines, correlating with shorter or longer periods. The <jats:italic>clock<\/jats:italic><jats:sup><jats:italic>crispr<\/jats:italic><\/jats:sup> lines also displayed distinct growth and developmental phenotypes that differ from those reported in the <jats:italic>Arabidopsis<\/jats:italic> clock mutant counterparts. Our transcriptomic analyses identified species\u2010specific regulation of key target genes. The results offer mechanistic insights into the conserved and divergent molecular pathways governing circadian phenotypic variations between <jats:italic>Arabidopsis<\/jats:italic> and tomato plants.<\/jats:p>","DOI":"10.1111\/tpj.70383","type":"journal-article","created":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T07:41:16Z","timestamp":1753774876000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Gene editing of clock components in <i>Solanum lycopersicum<\/i>: Effects on gene expression, development, and productivity"],"prefix":"10.1111","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7845-0058","authenticated-orcid":false,"given":"Benjamin","family":"Alary","sequence":"first","affiliation":[{"name":"Centre for Research in Agricultural Genomics (CRAG) CSIC, IRTA\u2010UAB\u2010UB, Campus UAB  Bellaterra 08193 Barcelona 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