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In addition to being one of the only fully self\u2010compatible diploid potato species,\n                    <jats:italic>S. verrucosum<\/jats:italic>\n                    is the only clade 4 species that lacks prezygotic interspecific reproductive barriers. This allows\n                    <jats:italic>S. verrucosum<\/jats:italic>\n                    to accept pollen from a broad range of\n                    <jats:italic>Solanum<\/jats:italic>\n                    species and thereby serving as a genetic \u201cbridge\u201d between the cultivated or primary potato gene pool and distantly related wild relatives in the tertiary gene pool. The genetic mechanisms underlying self\u2010compatibility in\n                    <jats:italic>Solanum<\/jats:italic>\n                    often underpin interspecific compatibility interactions, which in\n                    <jats:italic>S. verrucosum<\/jats:italic>\n                    , has been attributed to the lack of\n                    <jats:italic>S\u2010RNase<\/jats:italic>\n                    expression. Using an interspecific F2 mapping population (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u2009150), we investigated the genetic mechanisms responsible for the lack of interspecific reproductive barriers in\n                    <jats:italic>S. verrucosum<\/jats:italic>\n                    . This F2 population was evaluated for the ability to accept pollen from two clade 1, 1 EBN species (\n                    <jats:italic>S. pinnatisectum<\/jats:italic>\n                    and\n                    <jats:italic>S. tarnii<\/jats:italic>\n                    ); from which two QTL for interspecific compatibility were identified on chromosomes 1 and 11, explaining 56.6% of the phenotypic variation observed. To identify the genetic basis of interspecific compatibility, we generated a chromosome\u2010scale genome assembly of\n                    <jats:italic>S. verrucosum<\/jats:italic>\n                    MSII1813\u20102 and performed gene expression profiling of reproductive organs. Differential gene expression of\n                    <jats:italic>S\u2010RNase<\/jats:italic>\n                    , located within the chromosome 1 QTL, confirmed the central role of the\n                    <jats:italic>S<\/jats:italic>\n                    \u2010locus and specifically,\n                    <jats:italic>S\u2010RNase<\/jats:italic>\n                    , in interspecific compatibility. Discovery of a non\u2010\n                    <jats:italic>S<\/jats:italic>\n                    \u2010locus QTL is consistent with previous findings that other non\u2010\n                    <jats:italic>S<\/jats:italic>\n                    \u2010locus factors are necessary for interspecific compatibility in\n                    <jats:italic>S. verrucosum<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/tpj.70426","type":"journal-article","created":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T07:07:15Z","timestamp":1755760035000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Genetic basis for broad interspecific compatibility in\n                    <i>Solanum verrucosum<\/i>"],"prefix":"10.1111","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1757-9699","authenticated-orcid":false,"given":"William","family":"Behling","sequence":"first","affiliation":[{"name":"Department of Plant Soil and Microbial Sciences, Molecular Plant Science Michigan State University  1066 Bogue Street Room 2210 East Lansing Michigan 48824 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph","family":"Coombs","sequence":"additional","affiliation":[{"name":"Department of Plant Soil and Microbial Sciences, Molecular Plant Science Michigan State University  1066 Bogue Street Room 2210 East Lansing Michigan 48824 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thilanka","family":"Ranaweera","sequence":"additional","affiliation":[{"name":"Department of Plant Soil and Microbial Sciences, Molecular Plant Science Michigan State University  1066 Bogue Street Room 2210 East Lansing Michigan 48824 USA"},{"name":"Department of Plant Biology, Plant Biology Laboratories Michigan State University  612 Wilson Rd Room 166 East Lansing Michigan 48824 USA"},{"name":"Department of Energy (DOE) Great Lakes Bioenergy Research Center Michigan State University  East Lansing Michigan 48824 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brieanne","family":"Vaillancourt","sequence":"additional","affiliation":[{"name":"Center for Applied Genetic Technologies University of Georgia  111 Riverbend Rd. Room 249 Athens Georgia 30602 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John P.","family":"Hamilton","sequence":"additional","affiliation":[{"name":"Center for Applied Genetic Technologies University of Georgia  111 Riverbend Rd. Room 249 Athens Georgia 30602 USA"},{"name":"Department of Crop &amp; Soil Sciences University of Georgia  111 Riverbend Rd. Room 249 Athens Georgia 30602 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Brose","sequence":"additional","affiliation":[{"name":"Center for Applied Genetic Technologies University of Georgia  111 Riverbend Rd. Room 249 Athens Georgia 30602 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6727-4677","authenticated-orcid":false,"given":"C. Robin","family":"Buell","sequence":"additional","affiliation":[{"name":"Center for Applied Genetic Technologies University of Georgia  111 Riverbend Rd. Room 249 Athens Georgia 30602 USA"},{"name":"Department of Crop &amp; Soil Sciences University of Georgia  111 Riverbend Rd. Room 249 Athens Georgia 30602 USA"},{"name":"Institute of Plant Breeding, Genetics, &amp; Genomics University of Georgia  111 Riverbend Rd. Room 249 Athens Georgia 30602 USA"},{"name":"The Plant Center University of Georgia  111 Riverbend Rd. Room 249 Athens Georgia 30602 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David S.","family":"Douches","sequence":"additional","affiliation":[{"name":"Department of Plant Soil and Microbial Sciences, Molecular Plant Science Michigan State University  1066 Bogue Street Room 2210 East Lansing Michigan 48824 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00036761"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00021551"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-022-02823-7"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu638"},{"key":"e_1_2_7_7_1","unstructured":"Andrews S. Krueger F. Segonds\u2010Pichon A. Biggins L. 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