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The spliceosome is a large and highly dynamic molecular machine. Its core components include five small nuclear ribonucleoproteins (snRNPs) and the various spliceosome\u2010related proteins. The conserved Smith (Sm) complex and the Sm\u2010like proteins (LSm) serve as primary components of the snRNPs. Sm proteins are involved in processes such as pre\u2010mRNA splicing and mRNA degradation, which can regulate gene expression, thereby influencing plant growth, development, and stress responses. While 25 Sm proteins have been identified in rice, their specific roles in regulating rice growth and development remain unclear. In this study, we employed the CRISPR\/Cas9 system to edit 15 <jats:italic>OsSm<\/jats:italic> genes, and 13 mutants were obtained, with mutation rates ranging from 20.83 to 83.87%. In comparison to the wild type (WT), the mutants exhibited dwarfism, reduced tiller numbers, lower seed\u2010setting rates or sterility, and increased susceptibility to diseases. One Sm mutant, <jats:italic>ossmf\u20102<\/jats:italic>, exhibited dwarfism, delayed flowering, and small grains. Through transcriptome analysis, three target genes, <jats:italic>OsMRG702<\/jats:italic>, <jats:italic>OsRGG2<\/jats:italic>, and <jats:italic>OsLA1<\/jats:italic>, were identified. Mutations of the OsSmF protein may lead to the abnormal splicing of these genes and finally lead to the inhibition of growth and development. Our study first edited the <jats:italic>OsSm<\/jats:italic> genes and generated a mutant library in rice. Most of the mutants exhibited abnormal growth and development, underscoring the essential roles of OsSm proteins in rice physiology. Furthermore, this work addresses a critical gap in the functional characterization of Sm proteins in rice. The resulting mutant collection offers valuable germplasm resources and lays a theoretical foundation for elucidating the molecular regulatory networks involving spliceosomal components and their target genes in the control of crop growth, development, and reproduction.<\/jats:p>","DOI":"10.1111\/tpj.70475","type":"journal-article","created":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T23:10:20Z","timestamp":1759187420000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A <scp>CRISPR<\/scp>\/Cas9 mutant resource for OsSm <scp>RNA<\/scp>\u2010binding genes in rice"],"prefix":"10.1111","volume":"124","author":[{"given":"Xiongxia","family":"Jin","sequence":"first","affiliation":[{"name":"College of Tropical Agriculture and Forestry Hainan University  Haikou Hainan 570228 China"},{"name":"Institute of Nanfan &amp; Seed Industry Guangdong Academy of Sciences  Guangzhou Guangdong 510316 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Ouyang","sequence":"additional","affiliation":[{"name":"College of Tropical Agriculture and Forestry Hainan University  Haikou Hainan 570228 China"},{"name":"National Key Laboratory for Tropical Crop Breeding, College of Tropical Agriculture and Forestry Hainan University  Sanya Hainan 572025 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tong","family":"Sun","sequence":"additional","affiliation":[{"name":"CAS Center for Excellence in Molecular Plant Sciences Institute of Plant Physiology and Ecology, Chinese Academy of Sciences  Shanghai 200032 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cong","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Nanfan &amp; Seed Industry Guangdong Academy of Sciences  Guangzhou Guangdong 510316 China"},{"name":"Zhanjiang Research Center Institute of Nanfan &amp; Seed Industry, Guangdong Academy of Sciences  Zhanjiang Guangdong 524300 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7784-8120","authenticated-orcid":false,"given":"Jinbao","family":"Gu","sequence":"additional","affiliation":[{"name":"Institute of Nanfan &amp; Seed Industry Guangdong Academy of Sciences  Guangzhou Guangdong 510316 China"},{"name":"Zhanjiang Research Center Institute of Nanfan &amp; Seed Industry, Guangdong Academy of Sciences  Zhanjiang Guangdong 524300 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baoguang","family":"An","sequence":"additional","affiliation":[{"name":"Hainan Bolian Biotechnology Co., Ltd.  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