{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T08:49:22Z","timestamp":1790066962834,"version":"4.0.1"},"reference-count":67,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:00:00Z","timestamp":1762214400000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R01GM157611"],"award-info":[{"award-number":["R01GM157611"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    Meristems house pluripotent stem cells and sustain continuous growth and organogenesis in land plants. Unlike seed plants, whose gametophytes lack meristems, fern gametophytes initiate and maintain meristems, enabling growth independently from sporophytes. In the model fern\n                    <jats:italic>Ceratopteris richardii<\/jats:italic>\n                    , gametophytes develop into either hermaphrodites or males. Hermaphrodites maintain multicellular meristems and secrete the pheromone antheridiogen, directing undetermined gametophytes to become male. Under constant antheridiogen exposure, males lack meristems and exclusively produce sperm\u2010bearing antheridia. The phytohormone abscisic acid (ABA) antagonizes antheridiogen signaling during this process, suggesting a crucial, lineage\u2010specific role of ABA in fern meristem specification. Nonetheless, the cellular dynamics underlying ABA\u2010induced meristem formation and sex\u2010type conversion remain unclear. Here, we used non\u2010invasive, long\u2010term confocal time\u2010lapse imaging to capture the dynamic process of ABA\u2010induced male\u2010to\u2010hermaphrodite conversion in Ceratopteris at single\u2010cell resolution. Cell lineage analyses revealed that ABA triggers the formation of a\n                    <jats:italic>de novo<\/jats:italic>\n                    meristem, originating entirely from a single non\u2010antheridium meristem progenitor cell (MPC), mirroring the meristem formation observed following antheridiogen removal. Importantly, ABA exhibited dual functions: promoting the cell fate re\u2010specification essential for meristem initiation and concurrently suppressing cell division within the developing meristem lineage. Genetic analyses with different combinations of antheridiogen and ABA treatments demonstrated these dual roles could be uncoupled, yet both required functional ABA signaling. Our findings reveal both conserved and lineage\u2010specific mechanisms for meristem initiation triggered by distinct environmental cues, providing insight into hormone\u2010mediated cellular reprogramming and proliferation during sex\u2010type specification in land plants.\n                  <\/jats:p>","DOI":"10.1111\/tpj.70543","type":"journal-article","created":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T08:00:21Z","timestamp":1762243221000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Cellular basis of\n                    <scp>ABA<\/scp>\n                    \u2010induced\n                    <i>de novo<\/i>\n                    meristem formation and sex\u2010type conversion in Ceratopteris gametophytes"],"prefix":"10.1111","volume":"124","author":[{"given":"Xi","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Botany and Plant Pathology Purdue University  West Lafayette Indiana 47907 USA"},{"name":"Purdue Center for Plant Biology Purdue University  West Lafayette Indiana 47907 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9677-4470","authenticated-orcid":false,"given":"Xing","family":"Liu","sequence":"additional","affiliation":[{"name":"Purdue Center for Plant Biology Purdue University  West Lafayette Indiana 47907 USA"},{"name":"Department of Biochemistry Purdue University  West Lafayette Indiana 47907 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4105-5174","authenticated-orcid":false,"given":"Yun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Botany and Plant Pathology Purdue University  West Lafayette Indiana 47907 USA"},{"name":"Purdue Center for Plant Biology Purdue University  West Lafayette Indiana 47907 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,4]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2015.00100"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.120.7.1949"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.50.1.163"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1086\/297135"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genet-120215-035227"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13104-015-1193-x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/plcell\/koaf058"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.16.01848"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1893\/0005-3155(2007)78[58:COMGTH]2.0.CO;2"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2019.08.017"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-arplant-042809-112122"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/142.3.973"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2006.01787.x"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00220a083"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.117614"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evab042"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2024.06.064"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.15286"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-022-03627-y"},{"key":"e_1_2_9_21_1","article-title":"Confocal live imaging of shoot apical meristems from different plant species","volume":"145","author":"Geng Y.","year":"2019","journal-title":"JoVE, Journal of Visualized Experiments"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-16-6170-9_15"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2019.10.004"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2015.0490"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3790"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1139\/b83-098"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1086\/297255"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1537-2197.1987.tb08743.x"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.15067"},{"key":"e_1_2_9_30_1","first-page":"141","article-title":"A new classification of the gametophyte development of homosporous ferns, focusing on 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