{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:01:51Z","timestamp":1790697711026,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T00:00:00Z","timestamp":1765670400000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U22A20446"],"award-info":[{"award-number":["U22A20446"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Artemisia argyi<\/jats:italic>\n                    , a perennial herb of the Asteraceae family, possesses significant therapeutic and economic value. We present a 7.88\u2009Gb chromosome\u2010level haplotype\u2010resolved genome assembly, revealing its unique evolutionary trajectory. The karyotype (2\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200934) of\n                    <jats:italic>A. argyi<\/jats:italic>\n                    is that of an autotetraploid, which underwent gametic chromosome fusion prior to species\u2010specific whole\u2010genome duplication (WGD\u20103). The genome exhibits pronounced multivalent chromosome pairing and frequent recombination among homologous groups. Asymmetrical evolution following WGD\u20103 is a hallmark feature, evidenced by imbalanced allelic gene loss and widespread neofunctionalization. The\n                    <jats:italic>terpene synthase<\/jats:italic>\n                    (\n                    <jats:italic>TPS<\/jats:italic>\n                    ) gene family exemplifies this pattern, having expanded through four duplication events in\n                    <jats:italic>A. argyi<\/jats:italic>\n                    . Recent tandem duplications and allelic functional differentiation have generated substantial gene functional diversity. Notably, we identified a tandem\u2010duplicated six\u2010copy\n                    <jats:italic>ADS<\/jats:italic>\n                    homolog (\n                    <jats:italic>AarADS<\/jats:italic>\n                    )\u2014a key\n                    <jats:italic>TPS<\/jats:italic>\n                    gene in the artemisinin biosynthetic pathway of\n                    <jats:italic>Artemisia annua<\/jats:italic>\n                    (\n                    <jats:italic>AanADS<\/jats:italic>\n                    )\u2014localized exclusively to a single chromosome in\n                    <jats:italic>A. argyi<\/jats:italic>\n                    . Unlike AanADS, which converts farnesyl pyrophosphate (FPP) to amorpha\u20104,11\u2010diene, AarADS catalyzes FPP to \u03b1\u2010bisabolol. Evolutionary analysis suggested that AanADS acquired its specialized function via a derived mutation in the\n                    <jats:italic>A. annua<\/jats:italic>\n                    lineage. This study elucidates the genomic evolution underpinning\n                    <jats:italic>A. argyi<\/jats:italic>\n                    's distinctive medicinal properties.\n                  <\/jats:p>","DOI":"10.1111\/tpj.70548","type":"journal-article","created":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T21:19:43Z","timestamp":1765747183000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Comparative genomic analysis of\n                    <i>Artemisia argyi<\/i>\n                    reveals asymmetric expansion of terpene synthases and conservation of artemisinin biosynthesis"],"prefix":"10.1111","volume":"124","author":[{"given":"Xinlian","family":"Chen","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences Guangzhou University of Chinese Medicine  Guangzhou 510006 China"},{"name":"Key Laboratory of Chinese Medicinal Resource from Lingnan, Ministry of Education, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine  Guangzhou 510006 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baosheng","family":"Liao","sequence":"additional","affiliation":[{"name":"The Second Clinical College, Guangzhou University of Chinese Medicine  Guangzhou 510006 China"},{"name":"Key Laboratory of Chinese Medicinal Resource from Lingnan, Ministry of Education, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine  Guangzhou 510006 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duan","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Guangzhou University of Chinese Medicine  Guangzhou 510006 China"},{"name":"Key Laboratory of Chinese Medicinal Resource from Lingnan, Ministry of Education, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine  Guangzhou 510006 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyu","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Guangzhou University of Chinese Medicine  Guangzhou 510006 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengping","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Guangzhou University of Chinese Medicine  Guangzhou 510006 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihai","family":"Huang","sequence":"additional","affiliation":[{"name":"The Second Clinical College, Guangzhou University of Chinese Medicine  Guangzhou 510006 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lixin","family":"Duan","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Guangzhou University of Chinese Medicine  Guangzhou 510006 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2599-9079","authenticated-orcid":false,"given":"Qi","family":"Shen","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Guangzhou University of Chinese Medicine  Guangzhou 510006 China"},{"name":"Sanya Hospital of Traditional Chinese Medicine (Hainan Hospital, Guangzhou University of Chinese Medicine)  Sanya 572000 China"},{"name":"Key Laboratory of Chinese Medicinal Resource from Lingnan, Ministry of Education, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine  Guangzhou 510006 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature22380"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btl097"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2013.02.004"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/13880209.2010.497815"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.100101097"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.48130\/mpb\u20100024\u20100002"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xplc.2023.100516"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592\u2010020\u201001056\u20105"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010024\u201008476\u20109"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.17957\/IJAB\/15.1445"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aal3327"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2016.07.002"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102105\u20109\u201018"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2012.01.015"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.13"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10577\u2010019\u201009607\u2010z"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469\u20108137.2009.03117.x"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2022.121982"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010arplant\u2010050718\u2010100344"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2022.05.013"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2021.114404"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.33582\/2637\u20107721\/1002"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0914697107"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr011"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/agronomy13020436"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.13870"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcw094"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003\u2010021\u201002704\u2010y"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11295\u2010015\u20100937\u2010z"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.17.01310"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1438\u20108677.2009.00268.x"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059\u2010019\u20101650\u20102"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.3989\/collectbot.2011.v30.001"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.2307\/25065949"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.13930"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv351"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2018.11.029"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1159\/000351318"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg.2017.26"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr1293"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/gix093"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2022.11.015"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2019.03.046"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.4c08857"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-31442-1_3"}],"container-title":["The Plant Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tpj.70548","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/tpj.70548","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tpj.70548","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:07:44Z","timestamp":1790694464000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tpj.70548"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":45,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1111\/tpj.70548"],"URL":"https:\/\/doi.org\/10.1111\/tpj.70548","archive":["Portico"],"relation":{},"ISSN":["0960-7412","1365-313X"],"issn-type":[{"value":"0960-7412","type":"print"},{"value":"1365-313X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"2025-07-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70548"}}