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However, m\n                    <jats:sup>6<\/jats:sup>\n                    A dynamics, deposition and engineering in microalgae remain largely unknown. Here, in\n                    <jats:italic>Nannochloropsis oceanica<\/jats:italic>\n                    , the dynamic alterations and reprogramming in m\n                    <jats:sup>6<\/jats:sup>\n                    A RNA modifications after the shift from high to low CO\n                    <jats:sub>2<\/jats:sub>\n                    conditions were first investigated using methylated RNA immunoprecipitation sequencing. The m\n                    <jats:sup>6<\/jats:sup>\n                    A peaks in\n                    <jats:italic>N. oceanica<\/jats:italic>\n                    were mainly enriched in 3\u2032UTR. A positive association between m\n                    <jats:sup>6<\/jats:sup>\n                    A abundance and mRNA transcription of CO\n                    <jats:sub>2<\/jats:sub>\n                    \u2010responsive genes was observed; moreover,\n                    <jats:italic>N. oceanica<\/jats:italic>\n                    cells adopted versatile strategies in a dynamic reprogramming of m\n                    <jats:sup>6<\/jats:sup>\n                    A in response to low CO\n                    <jats:sub>2<\/jats:sub>\n                    stress. Secondly, knockout of two putative m\n                    <jats:sup>6<\/jats:sup>\n                    A methylases including NoMTA (NO04G02990) and NoMTB (NO07G02450) by genome editing induced methylation reprogramming, which was associated with expression changes of low\u2010CO\n                    <jats:sub>2<\/jats:sub>\n                    responsive genes such as carbon\/nitrogen metabolism, and photorespiration genes that underlie reductions in growth and biomass. Lastly, m\n                    <jats:sup>6<\/jats:sup>\n                    A modification reprogramming was first engineered to increase low\u2010CO\n                    <jats:sub>2<\/jats:sub>\n                    stress tolerance and biomass productivity by the CRISPR\/dCas13 system combined with MTA and NoMTB under low CO\n                    <jats:sub>2<\/jats:sub>\n                    in\n                    <jats:italic>N. oceanica<\/jats:italic>\n                    . Therefore, these strides would pave the way for microalgal epigenetics and future industrial applications.\n                  <\/jats:p>","DOI":"10.1111\/tpj.70566","type":"journal-article","created":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T16:50:53Z","timestamp":1762966253000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Epitranscriptomic reprogramming in response to low\n                    <scp>\n                      CO\n                      <sub>2<\/sub>\n                    <\/scp>\n                    stress and\n                    <scp>\n                      m\n                      <sup>6<\/sup>\n                      A\n                    <\/scp>\n                    engineering to enhance biomass production in\n                    <i>Nannochloropsis oceanica<\/i>"],"prefix":"10.1111","volume":"124","author":[{"given":"Han","family":"Zhu","sequence":"first","affiliation":[{"name":"Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences Hainan Normal University  Haikou 571158 China"},{"name":"Hainan Institute of National Park  Haikou 571100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yikai","family":"Wu","sequence":"additional","affiliation":[{"name":"Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences Hainan Normal University  Haikou 571158 China"},{"name":"Hainan Institute of National Park  Haikou 571100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Feng","sequence":"additional","affiliation":[{"name":"Ministry of Education Key Laboratory for 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